RESUMEN
The present work describes the bio-based green synthesis and characterization of zinc oxide nanoparticles (ZnO NPs) using leaf extract of Tridax procumbens; the synthesized nanoparticles were used to study their beneficial effect on the growth and metabolism of Vigna radiata. ZnO NPs were characterized using X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and ultraviolet-visible spectroscopy (UV-Vis spectra). Growth of V. radiata seedlings was measured in terms of shoot length and root length that were treated 20 and 40 mg/L concentrations of green synthesized ZnO NPs and constant concentration (50 mg/L) of PbCl2. These studies have shown the effect of ZnO NPs in the stimulation of growth as well as physiological and biochemical parameters. Vigna seedlings showed positive effects depending upon the increasing concentrations of ZnO NPs. This study suggests that ZnO NPs can be effectively used to ameliorate the toxicity of Pb in Vigna plants.
Asunto(s)
Nanopartículas del Metal , Vigna , Óxido de Zinc , Antibacterianos/química , Tecnología Química Verde , Nanopartículas del Metal/química , Nanopartículas del Metal/toxicidad , Pruebas de Sensibilidad Microbiana , Óxidos , Extractos Vegetales/química , Espectroscopía Infrarroja por Transformada de Fourier , Difracción de Rayos X , Óxido de Zinc/química , Óxido de Zinc/toxicidadRESUMEN
In the present study, zinc oxide nanoparticles (ZnO NPs) were rapidly synthesized at room temperature by treating zinc acetate dihydrate with the flower extract of Elaeagnus angustifolia (Russian olive). The formation of ZnO NPs was primarily confirmed by UV-visible absorption spectrum in the range of 250-700nm. XRD analysis and DLS particle size analyzer revealed the size of ZnO NPs. The FTIR spectrum revealed the presence of phytochemicals in the flower extract mediated ZnO NPs. Moreover, the morphology of the ZnO NPs was determined using SEM. Seeds of Solanum lycopersicum (tomato) were separately treated with different concentrations of synthesized ZnO NPs and zinc sulphate (ZnSO4) salt suspensions (common zinc supplement). The effect of these treatments on seed germination, seedling vigor, chlorophyll, protein and sugar contents as well as on the activities of lipid peroxidation and antioxidant enzyme were studied. Leaves of plants treated with 6.1mM concentration of ZnO NPs recorded maximum reflectance while it was minimum in plants treated with 1.2mM concentration of NPs. The effect of synthesized ZnO NPs on seedling vigor, pigment, protein and sugar content was found affirmative at lower concentrations contrary to control and ZnSO4 salt. The inhibitory effect at higher concentration of NPs indicated importance in the precise application of NPs, in Zn deficient system, where plant response varies with concentration. To the best of our knowledge this is the first report on Elaeagnus angustifolia mediated synthesis of ZnO NPs and their effects on germination and physiological activity of tomato.