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Objective:To compare the imaging quality and metabolic quantitative parameters of pulmonary nodules between Q. Flex whole information five-dimensional (5D) and conventional three-dimensional (3D) PET/CT imaging for clinical evaluation.Methods:Fifty-four patients (30 males, 24 females, age: 60(42, 75) years; 78 solid pulmonary nodules (maximum diameter≤3 cm) with abnormal uptake of 18F-FDG) from Tianjin Cancer Hospital Airport Hospital between June 2022 and August 2022 were enrolled in this retrospective study. All patients underwent 5D scanning and 3D, 5D reconstruction. Image quality scores, signal-to-noise ratio (SNR), SUV max, SUV mean and metabolic tumor volume (MTV) of pulmonary nodules of 5D group and 3D group were evaluated and compared with χ2 test and Wilcoxon signed rank test. Correlation of quantitative parameters between 2 groups were analyzed by using Spearman rank correlation analysis. Results:Thirty-five of 78(45%) pulmonary nodules with image quality score≥4 were found in 5D group, which were more than those in 3D group (22/78(28%); χ2=4.67, P=0.031). Meanwhile, SNR, SUV max, SUV mean, and MTV were significantly positively correlated between the 2 groups ( rs values: 0.86, 0.86, 0.85, and 0.95, all P<0.001). SNR, SUV max and SUV mean of pulmonary nodules in 5D group were significantly higher than those in 3D group, which were 37.46(18.42, 62.00) vs 32.72(16.97, 54.76) ( z=-4.07, P<0.001), 9.71(5.48, 13.82) vs 8.96(4.82, 12.63) ( z=-3.05, P<0.001) and 6.30(3.39, 8.94) vs 5.61(2.99, 7.63)( z=-4.07, P<0.001) respectively. MTV of pulmonary nodules in 5D group was significantly lower than that in 3D group, which was 1.72(0.66, 2.74) cm 3vs 1.98(1.06, 4.63) cm 3 ( z=-7.13, P<0.001). Quantitative parameters of lower lung field and nodules with maximum diameters of >10 mm and ≤20 mm based on 5D scanning changed most significantly compared with those based on 3D scanning ( z values: from -5.23 to -2.48, all P<0.05). Conclusion:Q. Flex 5D PET significantly improves the quantitative accuracy of SUV and MTV of pulmonary nodules, and the improvement of image quality is substantial without increasing the radiation dose, which has clinical practical value.
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Objective To explore the clinical value of serum interleukin(IL)-33 and its soluble receptor ST2(sST2) level in patients with chronic hepatitis B.Methods A total of 65 cases with chronic hepatitis B were recruited into study group,meanwhile 60 healthy persons were enrolled in the control group from January 2014 to October 2016 in our hospital.IL-33,sST2 and alanine aminotransferase(ALT) were detected and compared in the two groups.Results The level of IL-33,sST2 and ALT were significant higher than those of the control group(t=6.542,7.218,6.324;P<0.05).IL-33 and sST2 levels in chronic hepatitis B patients with abnormal ALT level were significant higher than those with normal ALT(t=16.328,9.874,P<0.05).Conclusion The detection of IL-33 and sST2 in patients with chronic hepatitis B could help to understand the immune status of patients,and provide a theoretical basis for the treatment of chronic hepatitis B.
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Objective To investigate the antigenicity of foreign antigen in recombinant vaccinia virus (VACV) after elimination of B8R,and provide help to improve the efficacy of VACV-based vaccines,and provide guidance for vaccine design.Methods Transfer vector pSC11-OVA was generated,and OVAgene was in,rted into VACV with B8R deletion.The biological characteristics of recombinant VACV was investigated in vitro,and the immune responses against OVA were tested in vivo.Results The plague phenotypes and growth of wcombinant VACV and its parental strains were essentially identical.Cellular immuneresponse against OVA was augmented in mice infected with B8R-deleted recombinant VACV when comparedwith those infected with B8R-intact recombinant VACV.Conclusion Deletion of B8R and insertion of OVAdoes not affect the biological characteristics of VACV.Immunogenicity of exogenous target antigens can beimproved in VACV with B8R deficiency.Deletion of dominant epitopes may provide a vector with more efficiency for vaccines and gene therapy.
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【Objective】To observe the effect of Sicaotang Decoction which is mainly composed of kidney-nourishing,toxicity-removing and blood-activating herbal medicines on parameters associated with mesenteric proliferation,fibronectin(FN),and Bcl-2 gene expression in lupus nephritis(LN).【Methods】 Sixty LN patients were equally randomized into two groups.The two groups received treatment with hormones and cyclophosphamide,and group Areceived Sicaotang Decoction additionally.The changes of Bcl-2 and Fas expression in CD4+ and CD8+ of T lymphocyte subtypes,serum fibronectin(FN),serum and urine collagen Ⅳ(CⅣ) as well as score of blood stasis were observed.【Results】Sicaotang Decoction reduced the expression of Bcl-2 and Fas expression in CD4+ and CD8+,relieved the proliferation of mesenteric cells,and decreased Bcl-2 positive rate,serum FN level,serum and urine CⅣ levels and score of blood stasis(P