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Objective To explore the role of miR-98 in peripheral blood mononuclear cells (PBMC) in the pathogenesis and development of childhood asthma.Methods A total of 43 cases of asthmatic children and 30 cases of healthy controls were enrolled in the study.Peripheral blood mononuclear cells were isolated in both healthy subjects and asthmatic children in acute attack and remission stages.The expressions of miR-98 and interleukin-4(IL-4) and IL-13 mRNA were detected by real-time quantitative PCR.Results The miR-98 levels of asthmatic children in attack stage were significantly lower than those in remission stage and control group (P0.05).Furthermore,a negative correlation was found between the expression of miR-98 and IL-5(r=-0.794,P<0.01) and between the expression of miR-98 and IL-13 mRNA (r=-0.804,P<0.01) in asthmatic children in attack stage.A positive correlation was also found between IL-4 and IL-13 mRNA in asthmatic children in attack stage (r=0.853,P<0.01).Conclusion The expression of miR-98 decreased in asthmatic children,and miR-98 might be involved in the pathogenesis and development of asthma.
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OBJECTIVE@#To survey and promote the ability of military men to treat burns.@*METHODS@#A total of 2200 military men were recruited to survey and examine their acknowledge and technique to treat burns, and then acknowledge and technique were taught to treat burns through multimedia and demonstration. One year later, the same subjects were surveyed again.@*RESULTS@#Before the intervention, their ability was deficient (the mean score was 51). Their scores were significantly promoted after the intervention (the mean score was 75, P0.01).@*CONCLUSION@#The ability of military men to treat burns needs to be improved for the potential hightech warfare.