RESUMEN
Different processed volatile oils from AS on urine metabolites of normal rats were analyzed to reveal the possible metabolic pathways. Totally 50 male Waster rats were randomly divided into normal control group, C-ASVO group, J-ASVO group, T-ASVO group and Y-ASVO group, with 10 rats in each group. The normal group was given isovolumetric 0.5% polyoxyethylene sorbitan fatty acid esterï¼Tween-80ï¼, while the other groups were given 0.176 mLâ¢kg⻹ different processed volatile oils from AS. Drugs were given for 3 successive days. The urine was collected at 48 h with metabolic cages. GC-MS was employed to detect the metabolic fingerprint of rat urine in different times. Principal component analysis(PCA) and orthogonal partial least-squares discriminant analysis(OPLS-DA) were adopted for a multivariate statistical analysis. Metabolites with potential differences were selected based on the results of variable importance in the projection(VIP) and t test. The metabolic pathway analysis(MetPA) database was built for different metabolites' metabolic pathways. The results showed that compared with the normal group, 31 kinds of endogenous metabolites in the different processed volatile oils from AS groups change significantly(P<0.05). And there were differences in normal rat urine metabolites among the different processed volatile oils from AS, of which the influence degree of J-ASVO was slightly stronger than C-ASVO, T-ASVO, and Y-ASVO. Therefore, the metabolism effect may be focused on energy metabolism, amino acid metabolism, fatty acid metabolism and glucose metabolism. This study focused on metabolism and mechanism of different processed volatile oils from AS, and provided new ideas for pharmacological actions of traditional Chinese medicines.
Asunto(s)
Angelica sinensis/química , Química Farmacéutica/métodos , Medicamentos Herbarios Chinos/administración & dosificación , Medicamentos Herbarios Chinos/química , Aceites Volátiles/administración & dosificación , Aceites Volátiles/química , Orina/química , Animales , Biomarcadores/orina , Medicamentos Herbarios Chinos/metabolismo , Cromatografía de Gases y Espectrometría de Masas , Masculino , Redes y Vías Metabólicas , Metabolómica , Aceites Volátiles/metabolismo , Ratas , Ratas WistarRESUMEN
Angelica sinensis (AS) is a well-known important traditional Chinese medicine that yields a volatile oil with anti-inflammatory effects. However, the holistic therapeutic effects and the mechanism underlying such effects of the volatile oil of A. sinensis (VOAS) are not yet well understood. Here, a gas chromatography-mass spectrometry-based metabonomic study was conducted to explore the significantly altered metabolites for better understanding of VOAS and to assess the integral efficacy of VOAS on a lipopolysaccharide (LPS)-induced inflammation rat model. Principal component analysis was used to investigate the global metabonomic alterations and to evaluate the therapeutic effects of VOAS in rats. Clear separations were observed in the comparison of the metabolite profiles of the normal control (NC) group, the LPS-stimulated group (MI), the VOAS group, and the dexamethasone (Dex) group. VOAS exerted therapeutic effects on the LPS-stimulated group, which were in accordance with the results of cytokine analyses and blood physiobiochemical assay. Furthermore, a total of 20, 17, and 22 metabolites distributed in 27 metabolic pathways were respectively identified in plasma, liver, and lung samples as significantly altered metabolites of MI, VOAS, Dex, and NC of the same background. Network analysis revealed that glycine, glutamate, malic acid, succinate, arachidonic acid, glycerol, galactose, and glucose were hub metabolites of the inflammation correlation network. Results indicated that VOAS exhibited an anti-inflammatory effect by adjusting the Krebs cycle, improving the glucose content, and restoring the fatty acid metabolism.