RESUMEN
Background: CMTM6 which is chemokine-like factor (CKLF)-like Marvel transmembrane domain containing family member 6 is involved in the occurrence and progression of various tumors. However, the role of CMTM6 is still unclear in lung adenocarcinoma (LUAD). Methods: Immunohistochemical, Western blotting and RTâPCR methods were used to detect the expression of CMTM6 in LUAD. Cox regression and the KaplanâMeier method were performed to assess overall survival. Immunogenic features were evaluated according to immune cell infiltrations, immune checkpoints. The sensitivity to chemotherapy agents was estimated using the pRRophetic package. Results: In LUAD, the expression of CMTM6 was obviously upregulated and was significantly associated with T stage (p = 0.008) and lymph node metastasis (p = 0.018). Multivariate Cox regression analysis demonstrated that CMTM6 was a specialty prognostic risk factor. Based on GSEA enrichment analysis, we found that high expression of CMTM6 is associated with multiple immune signaling pathways. The group with high CMTM6 expression showed a positive association with various types of tumor-infiltrating cells. Moreover, a total of 36 chemotherapeutic drugs were significantly correlated with the expression of CMTM6. Among them, two chemotherapeutic drugs had better therapeutic effects in the high CMTM6 expression group, while 34 chemotherapeutic drugs had therapeutic effects in the low CMTM6 expression group. Conclusion: This study confirmed that CMTM6 is highly expressed in LUAD and is a new independent poor prognostic factor. In addition, the high expression of CMTM6 is closely related to the tumor microenvironment and immunotherapy, providing new ideas for the treatment of posterior LUAD.
Asunto(s)
Adenocarcinoma del Pulmón , Neoplasias Pulmonares , Proteínas con Dominio MARVEL , Humanos , Adenocarcinoma del Pulmón/diagnóstico , Biomarcadores , Western Blotting , Neoplasias Pulmonares/diagnóstico , Microambiente Tumoral , Proteínas con Dominio MARVEL/metabolismoRESUMEN
Quality control of vaccine strains is directly associated with the safety and efficacy of inactivated whole bacterial vaccines. The assessment of genetic stability is one of the essential elements to guarantee the quality of vaccine strains. The multiple-valence inactivated leptospiral vaccine, comprising the main circulating serogroups, has played an important role in the control of Leptospira infection in China. In the present study, to assess the genetic stability of vaccine strains and develop novel quality control tests that enhance and extend the existing procedures, 7 Chinese leptospiral vaccine strains were characterized during in vivo and in vitro passages by multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) analysis. The seven vaccine strains were found to have distinct sequence types (STs) and PFGE profiles. Further analysis showed that the ST and PFGE pattern of each vaccine strain, after in vivo or serial in vitro passages (up to 20 passages), were identical to those of the initial strain, demonstrating that these strains were genetically stable and homogeneous. Taken together, PFGE and MLST provide a reproducible and reliable means for confirming the identity and genetic stability of vaccine seeds, suggesting that these approaches can be used to evaluate the quality of leptospiral vaccine strains.
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Vacunas Bacterianas/inmunología , Vacunas Bacterianas/normas , Electroforesis en Gel de Campo Pulsado , Leptospira/genética , Tipificación de Secuencias Multilocus , Tecnología Farmacéutica/métodos , Tecnología Farmacéutica/normas , Vacunas Bacterianas/genética , China , Variación Genética , Inestabilidad Genómica , Genotipo , Humanos , Leptospira/clasificación , Control de Calidad , Pase SeriadoRESUMEN
Inadvertent vaccine freezing often occurs in the cold chain and may cause damage to freezesensitive vaccines. Liquid vaccines that contain aluminum salt adjuvants are particularly vulnerable. Polyol cryoprotective excipients have been shown to prevent freeze damage to hepatitis B vaccine. In this study, we examined the freeze-protective effect of propylene glycol on diphtheria-tetanus-pertussis-whole-cell (DTwP) and acellular (DTaP) vaccines. Pilot lots of DTwP and DTaP formulated with 7.5% propylene glycol underwent 3 freeze-thaw treatments. The addition of propylene glycol had no impact on pH, particle size distribution, or potency of the vaccines prior to freeze-thaw treatment; the only change noted was an increase in osmolality. The potencies and the physical properties of the vaccines containing cryoprotectant were maintained after freeze-thawing and for 3 months in accelerated stability studies. The results from this study indicate that formulating vaccines with propylene glycol can protect diphtheria-tetanus-pertussis vaccines against freeze damages.
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Vacuna contra Difteria, Tétanos y Tos Ferina/química , Vacunas contra Difteria, Tétanos y Tos Ferina Acelular/química , Estabilidad de Medicamentos , Congelación , Tamaño de la Partícula , Propilenglicol/químicaRESUMEN
Monoclonal antibody (MAb)-based capture enzyme-linked immunosorbent assays (ELISA) were developed for the diagnosis of rabies-suspect specimens. A combination of four mouse monoclonal antibodies directed against the rabies virus nucleocapsid was selected and used for the detection. The test was optimized and standardized so that maximum concordance could be maintained with the standard procedures of rabies diagnosis recommended by the WHO expert committee. Using prototype viruses from the different genotypes of lyssavirus and from various geographic origins and phylogenetic lineages, this paper presents a reliable, rapid and transferable diagnostic method, named WELYSSA that readily permits the detection of lyssaviruses belonging to the 7 genotypes of lyssavirus circulating in Europe, Africa, Asia and Oceania. The threshold of detection of lyssavirus nucleocapsids is low (0.8 ng/ml). With a panel of 1030 specimens received for rabies diagnostic testing, this test was found to be highly specific (0.999) and sensitive (0.970) when compared to other recommended rabies diagnostic methods.
Asunto(s)
Ensayo de Inmunoadsorción Enzimática/métodos , Lyssavirus/aislamiento & purificación , Nucleocápside/análisis , Rabia/diagnóstico , Animales , Anticuerpos Monoclonales , Anticuerpos Antivirales , Encéfalo/virología , Ensayo de Inmunoadsorción Enzimática/estadística & datos numéricos , Genotipo , Humanos , Lyssavirus/clasificación , Lyssavirus/genética , Lyssavirus/inmunología , Ratones , Nucleocápside/inmunología , Rabia/inmunología , Rabia/virología , Virus de la Rabia/clasificación , Virus de la Rabia/genética , Virus de la Rabia/inmunología , Virus de la Rabia/aislamiento & purificación , Infecciones por Rhabdoviridae/diagnóstico , Infecciones por Rhabdoviridae/inmunología , Infecciones por Rhabdoviridae/virología , Sensibilidad y EspecificidadRESUMEN
OBJECTIVE: McAbs against rabies nucleocapsid were used to detect rabies street viruses in animal brain specimens with indirect immunofluorescent assay to evaluate the sensitivity and specificity of this assay. METHODS: 62 specimen from rabid animal brains including genotype 1 to 7 and 271 specimens from different normal animal brains collected in Pasteur Institute in 2003 were tested and compared, using indirect immunofluorescent assay. All these specimens were identified and compared using rapid rabies enzyme immunodiagnosis, fluorescent antibody test and rabies virus isolation assay in neuroblastoma cell culture which were all provided by Pasteur Institute. RESULTS: Both sensitivity and the specificity of the indirect immunofluorescent assay were 100%. CONCLUSION: The results showed a positive of rabies virus detection with these methods.