RESUMEN
Objective To achieve the goal of the qualitatively and relatively quantitatively analyze of the target pro-tein in a time-saving,labor-saving and reagents-saving way by the microfluidic paper-based immunoassay. Methods We chose MGMT as the target protein and compared the qualitative and semi-quantitative results of the MGMT ex-pression in the MCF7 cells which was treated with MGMT inhibitor, lomeguatrib, in both the traditional Western blot and the paper chip immunoassay. Results Microfluidic paper-based immunoassay can make the qualitative and relative quantitative detection on protein expression. Compared to the sensitivity of 1-5 ng of the traditional Western blot,the microfluidic paper-based immunoassay could detect as low as 10-25 pg of the protein. The sensitivity could be improved by 3 orders of magnitude. The entire operational duration took only 1 hour with less costed rea-gents being consumed. It maked the high-throughput protein detection as sensitive as the reverse phase protein as-says (RPPA) does. Conclusions The paper chip immunoassay could be performed qualitatively and semi-quanti-tatively to detect protein expression,and is more effective than that of traditional Western blot.
RESUMEN
Objective To achieve the goal of the qualitatively and relatively quantitatively analyze of the target pro-tein in a time-saving,labor-saving and reagents-saving way by the microfluidic paper-based immunoassay. Methods We chose MGMT as the target protein and compared the qualitative and semi-quantitative results of the MGMT ex-pression in the MCF7 cells which was treated with MGMT inhibitor, lomeguatrib, in both the traditional Western blot and the paper chip immunoassay. Results Microfluidic paper-based immunoassay can make the qualitative and relative quantitative detection on protein expression. Compared to the sensitivity of 1-5 ng of the traditional Western blot,the microfluidic paper-based immunoassay could detect as low as 10-25 pg of the protein. The sensitivity could be improved by 3 orders of magnitude. The entire operational duration took only 1 hour with less costed rea-gents being consumed. It maked the high-throughput protein detection as sensitive as the reverse phase protein as-says (RPPA) does. Conclusions The paper chip immunoassay could be performed qualitatively and semi-quanti-tatively to detect protein expression,and is more effective than that of traditional Western blot.
RESUMEN
Objective:To explain the present status of licensed pharmacists in China, and put forward suggestions for perfecting the system by a problem-focused method. Methods: The data from Certification Center for Licensed Pharmacists of CFDA and 2535 pieces of questionnaire from nationwide were reviewed, and the practice registration, distribution, educational background, qualifica-tions and the other status were analyzed. Results:Generally, the development of licensed pharmacists in China was promising. Howev-er, there were still some problems existing in China such as the lower registration rate compared with some countries abroad, the une-ven distribution inside, and the unclear duty understanding. Conclusion:It is proposed to formulate relevant policies to strengthen re-gional guidance, improve registration rate, distinguish the content of continuing education and so on in order to promote the quality of licensed pharmacists in China.