RESUMEN
Based on the anticancer mechanism of biological alkylating agent, we designed and synthesized two alpha pinene derivatives:(1R,5S)-(6,6-dimethylbicyclo[3,1,1]hept-2-en-2-yl)methyl benzenesulfonate and (1R,5S)-(6,6-dimethylbicyclo[3,1,1]hept-2-en-2-yl)methyl 4-methylbenzenesulfonate, of which structures were confirmed by ¹H-NMR, HPLC and MS date. These two compounds showed a good inhibition of tumor cells' proliferation. Further, the computer siuulation of molecular docking and metabolic kinetics indicated that these two copounds may have stable molecular complexation with protein CDK2, which closely related to the cell cycle.
Asunto(s)
Antineoplásicos/síntesis química , Diseño de Fármacos , Monoterpenos/síntesis química , Antineoplásicos/farmacología , Monoterpenos Bicíclicos , Línea Celular Tumoral , Proliferación Celular , Humanos , Simulación del Acoplamiento Molecular , Monoterpenos/farmacología , Relación Estructura-ActividadRESUMEN
This paper explored the method of resolving insufficient carbon source in urban sewage by comparing and analyzing denitrification and phosphorus removal (NPR) effect between modified two-sludge system and traditional anaerobic-aerobic-anoxic process under the condition of low carbon source wastewater. The modified two-sludge system was the experimental reactor, which was optimized by adding two stages of micro-aeration (aeration rate 0.5 L · mm⻹) in the anoxic period of the original two-sludge system, and multi-stage anaerobic-aerobic-anoxic SBR was the control reactor. When the influent COD, ammonia nitrogen, SOP concentration were respectively 200, 35, 10 mg · L⻹, the NPR effect of the experimental reactor was hetter than that of thecontrol reactor with the removal efficiency of TN being 94.8% vs 60.9%, and TP removal being 96.5% vs 75%, respectively. The effluent SOP, ammonia, TN concentration of the experimental reactor were 0.35, 0.50, 1.82 mg · L⻹, respectively, which could fully meet the first class of A standard of the Pollutants Emission Standard of Urban Wastewater Treatment Firm (GB 18918-2002). Using the optimized treatment process, the largest amounts of nitrogen and phosphorus removal per unit carbon source (as COD) were 0.17 g · g⻹ and 0.048 g · g⻹ respectively, which could furthest solve the lower carbon concentration in current municipal wastewater.
Asunto(s)
Carbono/química , Desnitrificación , Nitrógeno/aislamiento & purificación , Fósforo/aislamiento & purificación , Aguas del Alcantarillado/química , Purificación del Agua/métodos , Amoníaco/química , Reactores Biológicos , Aguas Residuales/químicaRESUMEN
Visible light responsive heterojunctions of graphitic carbon nitride (g-C3N4) and Bi2S3 were successfully designed and constructed by a simple solvothermal process. The as-prepared samples were characterized by X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), transmission electron microscopy (TEM), high resolution transmission electron microscopy (HRTEM) and UV-vis diffuse reflectance spectroscopy (DRS). Under visible light irradiation, the as-prepared g-C3N4/Bi2S3 photocatalysts exhibited highly enhanced photochemical efficiency in the degradation of methyl orange (MO) compared with pure g-C3N4 and Bi2S3. On the basis of the calculated energy bands, the excellent enhancement was attributed to the efficient separation of photoinduced electron-hole pairs. In addition, a detailed degradation pathway of MO degradation by g-C3N4/Bi2S3 composites was proposed to further elucidate the inner photodegradation mechanism. This research may provide a cost-effective and easy-scaling up approach to develop visible-light-driven photocatalysts, which could be applied in wastewater treatment.
Asunto(s)
Compuestos Azo/química , Luz , Fotólisis , CatálisisRESUMEN
Cervical cancer, mostly progressing from cervical intraepithelial neoplasia, is a major cause of morbidity and mortality in Chinese women. This is largely due to high prevalence of high-risk human papillomaviruses (hr-HPVs) in the population. The prevalence of hr-HPV DNA in women and in cervical lesions women ranged from 9.9% to 17.% and from 50.5% to 70.9% in different regions of China, respectively. The most common genotypes somewhat differ between regions throughout the country and from those in many other countries. This may be a challenge to cervical cancer screening and prevention in China. Combined detection of particular HPV genotypes should be recommended in all geographical regions in China and greater attention must be paid to specific hr-HPV types during cervical cancer screening and follow-up of cervical lesions. Besides, vaccination for prevention of cervical cancer by particular HPV genotypes, has not been introduced to China so far. Updated knowledge on prevalent HPV genotypes should be provided to public health organizations to help with the development of more effective HPV vaccines, which can protect Chinese women against HPV types prevalent in local China and thus have a substantial impact on the cervical cancer burden.
Asunto(s)
Detección Precoz del Cáncer/métodos , Pruebas de ADN del Papillomavirus Humano/métodos , Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/diagnóstico , Vacunas contra Papillomavirus/uso terapéutico , Displasia del Cuello del Útero/diagnóstico , Neoplasias del Cuello Uterino/diagnóstico , China , ADN Viral/genética , Femenino , Humanos , Papillomaviridae/genética , Infecciones por Papillomavirus/prevención & control , Infecciones por Papillomavirus/virología , Prevalencia , Factores de Riesgo , Neoplasias del Cuello Uterino/prevención & control , Neoplasias del Cuello Uterino/virología , Vacunación , Displasia del Cuello del Útero/prevención & control , Displasia del Cuello del Útero/virologíaRESUMEN
OBJECTIVE: To extract and separate toxic components from Phytolaccae Radix, and to comare the changes in toxicity of Phytolaccae Radix before and after being processed with vinegar. METHOD: The mucous membrane irritation response, mouse peritoneal inflammation model and in vitro macrophages release NO model were applied to compared the changes in inflammatory toxicity of toxic components from Phytolaccae Radix before and after being processed with vinegar. RESULT: Toxic components of Phytolacca Radix had significant inflammatory toxicity, which could cause conjunctival edema in rabbits, and increase of PGE2 and macrophages release NO content in peritoneal exudate in mice. After being processed with vinegar, they showed reduced irritation, which resulted in decrease of PGE2 and macrophages release NO content in peritoneal exudate in mice. CONCLUSION: After being processed with vinegar, the toxicity of toxic components from Phytolacca Radix decreased obviously.