RESUMEN
BACKGROUND: Acetylcholine induced inwardly rectifying current (I(KACh)) is a heteromultimeric complex formed by Kir3.1 and Kir3.4 subunits and plays important roles in the development of atrial fibrillation (AF). AF is a common disorder among Chinese, the frequency of AF is about 0.61%, and in patients with strokes it is 12.1%. We hypothesise that lone paroxysmal AF genetic variation in Kir3.4 may predispose the atria to fibrillation in the Chinese population. METHODS: We recruited 186 patients with lone paroxysmal AF, and 210 matched controls by age (49.61+/-8.04 years), sex, smoking habit, and left atrial dimension in Zhejiang Province, China. Genotype of Kir3.4 was determined with polymerase chain reaction (PCR) and direct sequencing. The SPSS statistical software was used for chi(2) test. LD and haplotypes were calculated using SHESIS software package. RESULTS: Three synonymous known single nucleotide polymorphisms (SNPs) in Kir3.4 were genotyped, including C171T (rs6590357), G810T (rs7118824) and C834T (rs7118833). We found low levels of linkage disequilibrium (LD) between C171T and G810T (D'=0.272), complete LD between SNPs G810T and C834T (D'=1) in AF patients and controls. The case-control analysis revealed that the frequency of genotype and allele in three SNPs are significantly different between lone paroxysmal AF patients than in control subjects. The odds ratio (OR) for AF 171T and 810T alleles were 1.546 (95% CI 1.015-2.355) and 1.520 (95% CI 1.012-2.284), respectively, when compared with patients without Kir3.4T alleles in these two loci. The OR for AF in patients with C-T genotype were 13.364 (95% CI 5.710-31.278) and 37.135 (95% CI 9.050-152.381) when comparing patients with T-G genotype. CONCLUSIONS: Our findings suggest that C171T and G810T SNPs in Kir3.4 gene might be risk factors for lone paroxysmal AF in Chinese population.
Asunto(s)
Fibrilación Atrial/genética , Canales de Potasio Rectificados Internamente Asociados a la Proteína G/genética , Polimorfismo de Nucleótido Simple/genética , Adulto , Estudios de Casos y Controles , China , Femenino , Frecuencia de los Genes , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Oportunidad RelativaRESUMEN
OBJECTIVE: To explore the detection rate of myocardial bridge (MB) in coronary angiography and its clinical significance. METHOD: The clinical data of 2 165 cases who had received selective coronary angiography were studied retrospectively. RESULTS: MB was detected in 66 patients (3. 0% ) , among whom 62 were found in left anterior descending artery. Among the 58 cases with isolated myocardial bridge, 24 had no remarkable symptoms, whereas 34 showed clinical symptoms such as angina. The arterial stenosis beneath MB was significantly correlated with clinical symptoms (P < 0. 05 ). CONCLUSION: The improvement of the detection rate of MB can effectively decrease misdiagnosis and benefit the treatment.