RESUMEN
Background: Endometrial cancer (EC) is an intractable gynecological cancer with increasing incidence and mortality worldwide. Accumulating studies indicated that long noncoding RNA nuclear enriched autosomal transcript 1 (NEAT1) was a novel oncogene implicated in a variety of cancers. However, whether NEAT1 could accelerate cell growth in EC is unclear. Materials and Methods: NEAT1, microRNA (miR)-202-3p, and T cell immunoglobulin and mucin domain 4 (TIMD4) levels were detected by quantitative real-time polymerase chain reaction. Cell proliferation and apoptosis were examined by cell counting kit-8 and flow cytometry. Transwell assay was employed for the evaluation of cell migration and invasion. The relationship between miR-202-3p and NEAT1 or TIMD4 was determined by luciferase reporter system. TIMD4 protein expression was assessed by Western blot assay. Results: NEAT1 was upregulated, whereas miR-202-3p was downregulated in EC tumors and cells. Depletion of NEAT1 restrained EC cell proliferation, migration, invasion, and improved apoptosis. MiR-202-3p was targeted by NEAT1 and could bind to TIMD4. Subsequently, it is observed that miR-202-3p inhibitor neutralized NEAT1 silencing mediated suppression on EC cell progression. Meanwhile, TIMD4 rescued miR-202-3p induced inhibition on cell progression in EC. Furthermore, it was obvious that NEAT1 facilitated TIMD4 expression by absorbing miR-202-3p in EC. Conclusions: Upregulation of NEAT1 accelerated EC cell progression through sponging miR-202-3p to facilitate TIMD4 expression, providing potential novel treatment method for EC.
Asunto(s)
Neoplasias Endometriales , Proteínas de la Membrana , MicroARNs , ARN Largo no Codificante , Femenino , Humanos , Apoptosis/genética , Línea Celular Tumoral , Movimiento Celular/genética , Proliferación Celular/genética , Neoplasias Endometriales/genética , Neoplasias Endometriales/patología , Inmunoglobulinas , MicroARNs/genética , MicroARNs/metabolismo , ARN Largo no Codificante/genética , ARN Largo no Codificante/metabolismo , Proteínas de la Membrana/genética , Proteínas de la Membrana/metabolismoRESUMEN
Identifying the transformations of heavy metal in different media is a scientific issue, and geographical detector is applied to evaluate the spatiotemporal stratified heterogeneity mechanisms for heavy metals in the Yangtze River Estuary. Heavy metal concentrations in water and sediment were consistent with lognormal distributions. Their concentrations were organized into four classes. Class 1 included concentrations that were less than or equal to 25%, Class 2 included those between 25%-50%, Class 3 concentrations were between 50%-75% and Class 4 were >75%, which were based on their lognormal distributions. In water and sediment, the mean heavy metal concentrations yearly decreased from 2012 to 2016. The Chongming area was significantly lower than those found in the other areas, which is the least affected area by anthropogenic activities. The explanatory power of sediment to spatiotemporal stratified heterogeneity of heavy metals in shellfish organisms was much greater than that of water.
Asunto(s)
Monitoreo del Ambiente , Estuarios , Metales Pesados/análisis , Contaminantes Químicos del Agua/análisis , Animales , Bivalvos/metabolismo , China , Sedimentos Geológicos/química , Metales Pesados/metabolismo , Ríos/química , Mariscos , Contaminantes Químicos del Agua/metabolismoRESUMEN
OBJECTIVE: To understand the relationship between human papillomavirus (HPV) genotype distribution and cervical neoplasia among women with abnormal cytology in Beijing. METHODS: In a study in Beijing, China, participants underwent a gynecologic examination between September 2006 and December 2009. Those with abnormal cytology underwent colposcopy, biopsy, and HPV testing. HPV was typed by flow-through hybridization on formalin-fixed paraffin-embedded tissue. RESULTS: Among 6339 women examined, 533 with abnormal cytology underwent HPV testing. The prevalence of high-risk HPV (HR-HPV) subtypes was 29.8%. HPV-16 was the most common genotype (12.8%), followed by HPV-33, HPV-58, HPV-66, HPV-18, and HPV-52. The cytologic and histopathologic grade clearly increased with a higher prevalence of HR-HPV infection. Both HPV-16 and HPV-33 were significantly associated with grade CIN2+ lesions (P<0.05). By contrast, HPV-18 and HPV-66 were not associated with cervical lesion severity. HR-HPV infection was most common among women aged 30-34 years (35.0%). CONCLUSION: HPV-16, HPV-58, HPV-33, HPV-66, HPV-18, and HPV-52 were the most common genotypes in Beijing. HPV-16 and HPV-33 might be associated with CIN2+ lesions. HPV-18 and HPV-66 were not associated with high-grade cervical lesions. The present results might help to improve HPV vaccination and cervical cancer prevention programs in Beijing.
Asunto(s)
Papillomaviridae/aislamiento & purificación , Infecciones por Papillomavirus/virología , Displasia del Cuello del Útero/virología , Adulto , Distribución por Edad , China/epidemiología , Colposcopía , Femenino , Flujo Génico , Genotipo , Humanos , Hibridación Genética , Persona de Mediana Edad , Papillomaviridae/genética , Infecciones por Papillomavirus/complicaciones , Infecciones por Papillomavirus/epidemiología , Prevalencia , Estudios Prospectivos , Displasia del Cuello del Útero/patologíaRESUMEN
The purpose of this study is to examine knowledge of cervical cancer and the relationship between knowledge of human papillomavirus (HPV) infection and cervical disease in a largely representative Beijing population. A questionnaire-based survey was conducted in a Beijing population sample of married women ages 25 to 54 years (n = 6,339), evaluating knowledge and relevant factors with regard to cervical cancer. Knowledge levels were low; only 52.5% (n = 3,328) of the respondents knew that cervical cancer can be detected in its early stage. Only 26.9% reported that human HPV infections were risk factors for cervical cancer. The level of knowledge was lower among older, with less education, less income, unstable profession, and non-Beijing Hukou women (all P < 0.01). An increasing level of knowledge may impact positively on intentions to participate in cervical cancer screening. Education schemes to improve knowledge of cervical cancer should be performed in a multimodel, multichannel method.
Asunto(s)
Conocimientos, Actitudes y Práctica en Salud , Tamizaje Masivo , Neoplasias del Cuello Uterino/diagnóstico , Neoplasias del Cuello Uterino/prevención & control , Adulto , Anciano , Escolaridad , Femenino , Humanos , Persona de Mediana Edad , Papillomaviridae , Infecciones por Papillomavirus/diagnóstico , Infecciones por Papillomavirus/prevención & control , Infecciones por Papillomavirus/virología , Factores de Riesgo , Encuestas y Cuestionarios , Neoplasias del Cuello Uterino/virologíaRESUMEN
OBJECTIVE: To determine the prevalence and determinants of the most commonly seen lower reproductive tract infections among women aged 25 - 54 years in Beijing. METHODS: The study population consisted of 6339 women aged 25 - 54 years in 137 communities of Beijing. Focus of this study was to understand the prevalence of the following diseases as:bacterial vaginosis, trichomoniasis and vulvovaginal candidiasis. In addition to their prevalence rates, a generalized equation for estimation was used to analyze those infection-associated factors. RESULTS: The overall infection prevalence in the lower reproductive tract was 11.4%, including bacterial vaginitis as 8.7%, trichomonads as 1.0% and vulvovaginal candidiasis as 1.7%. Factors which were found to be significantly associated with lower reproductive tract infections in women were age, profession, family income, number of sex partners and frequency of condom use during sexual contacts. In patients with bacterial vaginitis, both prevalence rates of cervical intraepithelial neoplasia and infection of human papillomavirus were high. CONCLUSION: The prevalence of the most commonly seen lower reproductive tract infections among women aged 25 - 54 years in Beijing was lower than other areas in China. Lower reproductive tract infections seemed to be related to 30 - 49 years of age, nongovernmental employee, poverty, higher number of sex partners and not using condoms during sexual contacts.