RESUMEN
OBJECTIVE: To understand the genotypes and nucleotide polymorphisms of Echinococcus granulosus metacestode from humans and sheep in Tianjun region, Qinghai Province. METHODS: The specific primers were designed according to the cox1 and nad1 genes of E. granulosus mitochondrial genome sequences accessed by GenBank. The primers were used to detect the cyst samples from 16 sheep and 2 humans infected with E. granulosus in Tianjun region of Qinghai Province by PCR, then the PCR amplification products were sequenced, the genotypes and nucleotide polymorphisms of the cox1 and nad1 genes were analyzed. RESULTS: The 18 isolated samples all belonged to E. granulosus G1 genotype. Among all the isolates, 9 haplotypes existed in the cox1 gene with 16 nucleotide mutation sites, and there were 0 to 5 nucleotide differences with the highest variation rate of 0.31%, whereas 7 haplotypes occurred with 15 nucleotide mutation sites, and there were 1 to 8 nucleotide differences with the highest variation rate of 0.89% for the nad1 gene. CONCLUSIONS: The epidemic genotype of E. granulosus is G1 in humans and sheep in Tianjun region of Qinghai Province, and the nucleotide polymorphisms of the cox1 gene were more abundant than those of the nad1 gene, and the resolution of the nucleotide polymorphisms of cox1 gene is higher than that of the nad1 gene used in E. granulosus isolates.
Asunto(s)
Equinococosis/parasitología , Echinococcus granulosus/genética , Ovinos/parasitología , Animales , China , Análisis Mutacional de ADN , ADN de Helmintos/genética , Genes de Helminto , Genotipo , Haplotipos , Humanos , FilogeniaRESUMEN
Objective To understand the genotypes and nucleotide polymorphisms of Echinococcus granulosus metacestode from humans and sheep in Tianjun region,Qinghai Province. Methods The specific primers were designed according to the cox1 and nad1 genes of E.granulosus mitochondrial genome sequences accessed by GenBank.The primers were used to detect the cyst samples from 16 sheep and 2 humans infected with E.granulosus in Tianjun region of Qinghai Province by PCR,then the PCR amplification products were sequenced,the genotypes and nucleotide polymorphisms of the cox1 and nad1 genes were analyzed.Results The 18 isolated samples all belonged to E.granulosus G1 genotype.Among all the isolates,9 haplotypes ex-isted in the cox1 gene with 16 nucleotide mutation sites,and there were 0 to 5 nucleotide differences with the highest variation rate of 0.31%,whereas 7 haplotypes occurred with 15 nucleotide mutation sites,and there were 1 to 8 nucleotide differences with the highest variation rate of 0.89% for the nad1 gene.Conclusions The epidemic genotype of E.granulosus is G1 in hu-mans and sheep in Tianjun region of Qinghai Province,and the nucleotide polymorphisms of the cox1 gene were more abundant than those of the nad1 gene,and the resolution of the nucleotide polymorphisms of cox1 gene is higher than that of the nad1 gene used in E.granulosus isolates.
RESUMEN
<p><b>OBJECTIVE</b>Using doubly labeled water method to validate the colmputer science application's activity monitor (CSA) in assessing physical activity of free-living adults in Beijing, in order to develop equations to predict total daily energy expenditure (TEE) and activity related energy expenditure (AEE) from activity counts (AC) and anthropometric variables.</p><p><b>METHODS</b>A total of 72 healthy adults (33 males and 39 females, mean age 43.6 +/- 4.0 yr) were monitored for 7 consecutive days by CSA. TEE was simultaneously measured using doubly labeled water method. Average AC (counts/min(-1)) was compared with TEE, AEE and physical activity level (PAL).</p><p><b>RESULTS</b>Physical activity determined by AC was significantly related to data on energy expenditures: TEE (r = 0.31, P < 0.01), AEE (r = 0.30, P < 0.05), and PAL (r = 0.26, P < 0.05). Multiple stepwise regression analysis showed that TEE was significantly influenced by gender, fat-free mass (FFM) or BMI and AC (R(2) = 0.52 - 0.70) while AEE was significantly influenced by gender, FFM and AC (R(2) = 0.25 - 0.32).</p><p><b>CONCLUSION</b>AC from CSA activity monitor seemed a useful measure in studying the total amount of physical activity in free-living adults while AC significantly contributed to the explained variation in TEE and AEE.</p>