RESUMEN
Aerobic composting is a promising technology for converting manure into organic fertilizer with low capital investment and easy operation. However, the large nitrogen losses in conventional aerobic composting impede its development. Interconversion of nitrogen species was studied during membrane-covered aerobic composting (MCAC) and conventional aerobic composting, and solid-, liquid-, and gas-phase nitrogen migration pathways were identified by performing nitrogen balance measurements. During the thermophilic phase, nitrogenous organic matter degradation and therefore NH3 production were faster during MCAC than uncovered composting. However, the water films inside and outside the membrane decreased NH3 release by 13.92%-22.91%. The micro-positive pressure environment during MCAC decreased N2O production and emission by 20.35%-27.01%. Less leachate was produced and therefore less nitrogen and other pollutants were released during MCAC than uncovered composting. The nitrogen succession patterns during MCAC and uncovered composting were different and NH4+ storage in organic nitrogen fractions was better facilitated during MCAC than uncovered composting. Overall, MCAC decreased total nitrogen losses by 33.24%-50.07% and effectively decreased environmental pollution and increased the nitrogen content of the produced compost.
Asunto(s)
Compostaje , Contaminantes Ambientales , Nitrógeno/análisis , Suelo , EstiércolRESUMEN
Pollutants in livestock manure have a compound effect during aerobic composting, but research to date has focused more on single factors. This study investigated the effects of adding amoxicillin (AMX), copper (Cu) and both (ACu) on nitrogen transformation and the microbial mechanisms in cow manure aerobic composting with wheat straw. In this study, compared with CK, AMX, Cu, and ACu increased NH3 cumulative emissions by 32.32%, 41.78% and 8.32%, respectively, due to their inhibition of ammonia oxidation. Coexisting AMX and Cu decreased the absolute abundances of amoA/ nxrA genes and increased the absolute abundances of nirS /nosZ genes, but they had an antagonistic effect on the changes in functional gene abundances. Pseudomonas and Luteimonas were enriched during the thermophilic and cooling periods due to the addition of AMX and ACu, which enhanced denitrification in these two groups. Moreover, adding AMX and/or Cu led to more complex bacterial networks, but the effect of the two pollutants was lower than those of the individual pollutants. These findings provide theoretical and experimental support for controlling typical combined pollution with antibiotics and heavy metals in livestock manure.
Asunto(s)
Amoxicilina , Compostaje , Animales , Bovinos , Amoxicilina/farmacología , Estiércol , Cobre , Nitrógeno , SueloRESUMEN
Aerobic composting is a humification process accompanied by nitrogen loss. This study is the first research systematically investigating and elucidating the mechanism by which functional membrane-covered aerobic composting (FMCAC) reduces nitrogen loss and enhances humification. The variations in bioavailable organic nitrogen (BON) and humic substances (HSs) in different composting systems were quantitatively studied, and the functional succession patterns of fungal groups were determined by high-throughput sequencing and FUNGuild. The FMCAC improved oxygen utilization and pile temperature, increased BON by 29.95 %, reduced nitrogen loss by 34.00 %, and enhanced humification by 26.09 %. Meanwhile, the FMCAC increased the competitive advantage of undefined saprotroph and significantly reduced potential pathogenic fungi (<0.10 %). Structural equation modeling indicated that undefined saprotroph facilitated the humification process by increasing the production of BON and storing BON in stable humic acid. Overall, the FMCAC increased the safety, stability, and quality of the final compost product.
Asunto(s)
Compostaje , Nitrógeno/análisis , Sustancias Húmicas/análisis , Temperatura , Hongos , Suelo , EstiércolRESUMEN
Carbon and nitrogen evolution and bacteria and fungi succession in two functional membrane-covered aerobic composting (FMCAC) systems and a conventional aerobic composting system were investigated. The micro-positive pressure in each FMCAC system altered the composting microenvironment, significantly increased the oxygen uptake rates of microbes (p < 0.05), and increased the abundance of cellulose- and hemicellulose-degrading microorganisms. Bacteria and fungi together influenced the conversion between carbon and nitrogen forms. FMCAC made the systems less anaerobic and decreased CH4 production and emissions by 22.16 %-23.37 % and N2O production and emissions by 41.34 %-45.37 % but increased organic matter degradation and NH3 production and emissions by 16.91 %-90.13 %. FMCAC decreased carbon losses, nitrogen losses, and the global warming potential by 7.97 %-11.24 %, 15.43 %-34.00 %, and 39.45 %-42.16 %, respectively. The functional membrane properties (pore size distribution and air permeability) affected fermentation process and gaseous emissions. A comprehensive assessment indicated that FMCAC has excellent prospects for application.
Asunto(s)
Compostaje , Micobioma , Carbono , Nitrógeno/análisis , Bacterias , Suelo , EstiércolRESUMEN
This study investigated the effect of biochar on antibiotics and antibiotic resistance genes (ARGs) during aerobic composting of pig manure. First, the composition and content of antibiotics in the manure were determined qualitatively and quantitatively. Biochar promoted the degradation of these antibiotics (oxytetracycline, chlortetracycline, and tetracycline). The relative abundance (RA) of antibiotic-resistant bacteria carrying ARGs accounted for about 29.32% of the total bacteria. Firmicutes and Actinomycetes were dominant phylum-level bacteria at the early and late stages of composting, respectively. Biochar decreased the total RA of ARGs by 16.83%±4.10%. tetW and tetL, closely related to tetracycline resistance, were significantly diminished during aerobic composting, and biochar was able to promote this removal. Biochar enhanced RAs of Mycobacterium tuberculosis kasA mutant. RAs of ARGs related to antibiotic efflux pumps, such as baeS and arlS, remained at a high level. Conclusively, biochar promotes degradation of antibiotics and removal of ARGs.
Asunto(s)
Compostaje , Animales , Antibacterianos/farmacología , Bacterias/genética , Carbón Orgánico , Genes Bacterianos/genética , Estiércol/microbiología , Metabolómica , Metagenómica , PorcinosRESUMEN
This study investigated and assessed the effect of the functional-membrane covering technique (FMCT) on nitrogen succession during aerobic composting. By comparative experiments involving high-throughput sequencing and qPCR, nitrogen metabolism (including the ko00910 pathway and functional enzyme and gene abundances) was analyzed, and the nitrogen succession mechanism was identified. The FMCT created a micro-positive pressure, improved the aerobic conditions, and increased the oxygen utilization rate and temperature. This strongly affected the nitrogen metabolism pathway and down-regulated the nitrifying and denitrifying bacteria abundances. The FMCT up-regulated the relative abundance of glutamate dehydrogenase and down-regulated the absolute abundances of AOB and nxrA. This and the high temperature increased NH3 emissions by 13.78%-73.37%. The FMCT down-regulated the abundances of denitrifying gene groups (nirS + nirK)/nosZ and nitric oxide reductase associated with N2O emissions and decreased N2O emissions by 16.44%-41.15%. The results improve the understanding of the mechanism involved in nitrogen succession using the FMCT.
Asunto(s)
Compostaje , Desnitrificación , Redes y Vías Metabólicas , Nitrógeno/análisis , Óxido Nitroso/análisis , Suelo , Microbiología del SueloRESUMEN
This study evaluated the greenhouse gas emissions of solid dairy manure storage with the micro-aerobic group (MA; oxygen concentration <5%) and control group (CK; oxygen concentration <1%), and explained the difference in greenhouse gas emissions by exploring bacterial community succession. The results showed that the MA remained the micro-aerobic conditions, which the maximum and average oxygen concentrations were 4.1% and 1.9%, respectively; while the average oxygen concentrations of the CK without intervention management was 0.5%. Compared with the CK, carbon dioxide and methane emissions in MA were reduced by 78.68% and 99.97%, respectively, and nitrous oxide emission was increased by almost three times with a small absolute loss, but total greenhouse gas emissions decreased by 91.23%. BugBase analysis showed that the relative abundance of aerobic bacteria in CK decreased to 0.73% on day 30, while that in MA increased to 6.56%. Genus MBA03 was significantly different between the two groups (p < 0.05) and was significantly positively correlated with carbon dioxide and methane emissions (p < 0.05). A structural equation model also revealed that the oxygen concentration and MBA03 of the MA had significant direct effects on methane emission rate (p < 0.001). The research results could provide theoretical basis and measures for directional regulation of greenhouse gas emission reduction during dairy manure storage.
Asunto(s)
Gases de Efecto Invernadero , Dióxido de Carbono/análisis , Efecto Invernadero , Gases de Efecto Invernadero/análisis , Estiércol/análisis , Metano/análisis , Óxido Nitroso/análisisRESUMEN
The effects of the functional membrane covering on the gas emissions and bacterial community during dairy cow manure aerobic composting were investigated. A lab-scale aerobic composting experiment was conducted with the control group (CK), Gore group (Gore), and ZT group (ZT), namely, without and with two functional membranes. Covering the functional membrane retained heat and improved the seed germination index in Gore and ZT groups. Compared with the CK group, the Gore membrane decreased NH3 and N2O emissions by 11.77% and 26.40%, respectively. The ZT membrane decreased N2O and CO2 emissions by 68.44% and 1.56%, respectively. The Gore and ZT membranes decreased the global warming potential by 16.97% and 53.41%, respectively. Moreover, Covering the two functional membranes improved the Actinobacteria relative abundance and were conducive to the degradation of volatile solid. Altogether, membrane-covered aerobic composting is an important technology for the resource utilization of organic waste.
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Compostaje , Bacterias , Estiércol , SueloRESUMEN
The effects of functional membrane covering (FMC) on nitrogen transformation and related functional genes during aerobic composting were investigated by performing a comparable experiment. The FMC increased the pile temperature, promoted compost maturity, and decreased nitrogen loss. The FMC reduced NH3 and N2O emissions by 7.34% and 26.27%, respectively. The water film and the micro-positive pressure environment under the membrane effectively prevented NH3 escaping. The FMC up-regulated the amoA gene copy number (promoting NH3/NH4+ oxidation). The reduction of N2O emission by the FMC was mainly related to denitrifying genes (nirK, nirS, and nosZ). The FMC down-regulated the nirK and nirS gene copy numbers, but up-regulated the nosZ gene copy number. Pearson correlation analysis indicated that the functional membrane characteristics and differences between the composting pile environments caused by the FMC significantly affected the nitrogen forms and the related functional genes. The FMC strongly decreased nitrogen emissions and therefore conserved nitrogen.
Asunto(s)
Compostaje , Desnitrificación , Estiércol , Nitrógeno , Óxido Nitroso/análisis , SueloRESUMEN
To investigate the emission mechanism of ammonia (NH3) and nitrous oxide (N2O) during aerobic composting and the influence of powder bamboo biochar (PBB) on this process, this paper conducted a systematic study on the nitrogen-transforming functional microbial community, including functional genes, microbial structure and metabolism pathways. PBB reduced N2O and NH3 emissions by 1.25%-8.72% and 10.4%-11.8%, respectively. The quantitative PCR results indicated that the reduced N2O emission by PBB were mainly related to denitrifying genes (nirS, nirK, nosZ, and narG). The metagenome results demonstrated that Nitrosococcus was the main genus that could oxidize ammonia to nitrite decreased by PBB. The PBB significantly affected the nitrogen metabolism pathway, reduced the activity of glutamate dehydrogenase to inhibit the formation of NH4+ to reduce NH3 emission. The higher N2O emission in the control group was also related to the higher relative contents of hydroxylamine reductase and nitrite reductase.