RESUMEN

The role of several feline vector-borne pathogens (FVBP) as a cause of disease in cats has not been clearly determined. In fact, with the exception of Bartonella spp. and hemoplasmas, FVBP in cats has not been clearly determined in Brazil yet. The present study aimed at identifying, by using molecular methods, the presence of FVBP in three cats showing non-specific clinical signs and inclusions suggestive of hemoparasites in blood smears. Cytauxzoon felis, Candidatus Mycoplasma haemominutum, Ehrlichia sp. closely related to Ehrlichia canis, and Anaplasma sp. closely related to Anaplasma phagocytophilum were detected in blood samples from two out of three sampled cats. Both cats positive for multiple FVBP did not show hematological and biochemical abnormalities. The present work emphasizes the need for molecular confirmation of co-infection by multiple FVBP in cats presenting non-specific clinical signs and inclusions resembling hemoparasites in blood smears.(AU)

O papel de diversos patógenos felinos transmitidos por vetores (PFTV) como causa de enfermidades em gatos não tem sido claramente determinado. De fato, com exceção de Bartonella spp. e hemoplasmas, PFTV têm sido bem menos estudados no Brasil. O presente estudo objetivou investigar, utilizando métodos moleculares, a presença de PFTV em três gatos apresentando sinais clínicos inespecíficos e inclusões sugestivas de hemoparasitas em esfregaços sanguíneos. Cytauxzoon felis, Candidatus Mycoplasma haemominutum, Ehrlichia sp. filogeneticamente relacionada a Ehrlichia canis, e Anaplasma sp. filogeneticamente relacionado a Anaplasma phagocytophilum foram detectados em amostras de sangue de dois dos três gatos amostrados. Os dois gatos positivos para múltiplos PFTV não apresentaram alterações hematológicas e bioquímicas. O presente trabalho enfatiza a necessidade de confirmação molecular da infecção por múltiplos PFTV em gatos apresentando sinais clínicos inespecíficos e inclusões sugestivas de hemoparasitas em esfregaços sanguíneos.(AU)

Asunto(s)

RESUMEN

Abstract The role of several feline vector-borne pathogens (FVBP) as a cause of disease in cats has not been clearly determined. In fact, with the exception of Bartonella spp. and hemoplasmas, FVBP in cats has not been clearly determined in Brazil yet. The present study aimed at identifying, by using molecular methods, the presence of FVBP in three cats showing non-specific clinical signs and inclusions suggestive of hemoparasites in blood smears. Cytauxzoon felis, 'Candidatus Mycoplasma haemominutum', Ehrlichia sp. closely related to Ehrlichia canis, and Anaplasma sp. closely related to Anaplasma phagocytophilum were detected in blood samples from two out of three sampled cats. Both cats positive for multiple FVBP did not show hematological and biochemical abnormalities. The present work emphasizes the need for molecular confirmation of co-infection by multiple FVBP in cats presenting non-specific clinical signs and inclusions resembling hemoparasites in blood smears.

Resumo O papel de diversos patógenos felinos transmitidos por vetores (PFTV) como causa de enfermidades em gatos não tem sido claramente determinado. De fato, com exceção de Bartonella spp. e hemoplasmas, PFTV têm sido bem menos estudados no Brasil. O presente estudo objetivou investigar, utilizando métodos moleculares, a presença de PFTV em três gatos apresentando sinais clínicos inespecíficos e inclusões sugestivas de hemoparasitas em esfregaços sanguíneos. Cytauxzoon felis, 'Candidatus Mycoplasma haemominutum', Ehrlichia sp. filogeneticamente relacionada a Ehrlichia canis, e Anaplasma sp. filogeneticamente relacionado a Anaplasma phagocytophilum foram detectados em amostras de sangue de dois dos três gatos amostrados. Os dois gatos positivos para múltiplos PFTV não apresentaram alterações hematológicas e bioquímicas. O presente trabalho enfatiza a necessidade de confirmação molecular da infecção por múltiplos PFTV em gatos apresentando sinais clínicos inespecíficos e inclusões sugestivas de hemoparasitas em esfregaços sanguíneos.

Asunto(s)

RESUMEN

The role of several feline vector-borne pathogens (FVBP) as a cause of disease in cats has not been clearly determined. In fact, with the exception of Bartonella spp. and hemoplasmas, FVBP in cats has not been clearly determined in Brazil yet. The present study aimed at identifying, by using molecular methods, the presence of FVBP in three cats showing non-specific clinical signs and inclusions suggestive of hemoparasites in blood smears. Cytauxzoon felis, 'Candidatus Mycoplasma haemominutum', Ehrlichia sp. closely related to Ehrlichia canis, and Anaplasma sp. closely related to Anaplasma phagocytophilum were detected in blood samples from two out of three sampled cats. Both cats positive for multiple FVBP did not show hematological and biochemical abnormalities. The present work emphasizes the need for molecular confirmation of co-infection by multiple FVBP in cats presenting non-specific clinical signs and inclusions resembling hemoparasites in blood smears.

Asunto(s)

RESUMEN

Canine lymphoma is a hematopoietic neoplasm that can affect different tissues. Its classification is performed according to cellular morphology, clinical stage, immunophenotyping, and anatomical location. However, in some cases, an oncopathy might be in an advanced stage with a plain organic spread, making it impossible to determine the initially affected tissue, which is a rare situation in animals. Thus, a case of metastatic lymphoma, of unknown primary origin, was described in a four-year-old Rottweiler bitch with multiple nodular subcutaneous proliferations. Hematological, biochemical, thoracic radiography, and abdominal ultrasound tests were performed, as well as a cytopathological examination of subcutaneous lesions and bone marrow. Hematologic findings determined anemia, lymphopenia, and thrombocytopenia. The thoracic radiography revealed multiple nodular masses. Cytopathological examination revealed infiltration of malignant lymphocytes into the subcutaneous nodules and into the bone marrow. The dog was euthanized and submitted to necroscopic examination, in which multiple nodules were found in the subcutaneous region, lung, pericardium, gastrointestinal tract and in the skeletal musculature, in addition to lymphadenopathy. Fragments of the affected structures were collected for later histopathological and immunohistochemical evaluation. Histopathological examination revealed the presence of neoplastic cells compatible with diffuse lymphoma, which was positively immunolabelled for T-cell lymphoma. Based on clinical-pathological findings and other complementary tests, a diagnosis of diffuse T-cell lymphoma of unknown primary origin was made. In order to classify hematopoietic neoplasms, it is essential to perform different laboratory tests. However, determination of origin becomes difficult when such an oncological disease presents an advanced stage.(AU)

Asunto(s)

RESUMEN

The role of several feline vector-borne pathogens (FVBP) as a cause of disease in cats has not been clearly determined. In fact, with the exception of Bartonella spp. and hemoplasmas, FVBP in cats has not been clearly determined in Brazil yet. The present study aimed at identifying, by using molecular methods, the presence of FVBP in three cats showing non-specific clinical signs and inclusions suggestive of hemoparasites in blood smears. Cytauxzoon felis, Candidatus Mycoplasma haemominutum, Ehrlichia sp. closely related to Ehrlichia canis, and Anaplasma sp. closely related to Anaplasma phagocytophilum were detected in blood samples from two out of three sampled cats. Both cats positive for multiple FVBP did not show hematological and biochemical abnormalities. The present work emphasizes the need for molecular confirmation of co-infection by multiple FVBP in cats presenting non-specific clinical signs and inclusions resembling hemoparasites in blood smears.(AU)

O papel de diversos patógenos felinos transmitidos por vetores (PFTV) como causa de enfermidades em gatos não tem sido claramente determinado. De fato, com exceção de Bartonella spp. e hemoplasmas, PFTV têm sido bem menos estudados no Brasil. O presente estudo objetivou investigar, utilizando métodos moleculares, a presença de PFTV em três gatos apresentando sinais clínicos inespecíficos e inclusões sugestivas de hemoparasitas em esfregaços sanguíneos. Cytauxzoon felis, Candidatus Mycoplasma haemominutum, Ehrlichia sp. filogeneticamente relacionada a Ehrlichia canis, e Anaplasma sp. filogeneticamente relacionado a Anaplasma phagocytophilum foram detectados em amostras de sangue de dois dos três gatos amostrados. Os dois gatos positivos para múltiplos PFTV não apresentaram alterações hematológicas e bioquímicas. O presente trabalho enfatiza a necessidade de confirmação molecular da infecção por múltiplos PFTV em gatos apresentando sinais clínicos inespecíficos e inclusões sugestivas de hemoparasitas em esfregaços sanguíneos.(AU)

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