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1.
Int J Biol Macromol ; 271(Pt 1): 132570, 2024 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-38782316

RESUMEN

Resistant starch (RS) is important in controlling diabetes. The primary objective of this study is to examine the impact of molecular conformation on the enzymatic hydrolysis efficiency of starch by α-amylase. And the interactions between starch molecules with different conformations and α-amylase were analysed by using molecule dynamics simulation and molecular docking. It was found, the natural conformational starch molecule was hydrolysed from the middle of the starch chain by α-amylase, producing polysaccharides. The bent PS-conformational starch molecules with multiple O2-O3 intramolecular hydrogen bonds produced by high-pressure was hydrolysed from the head of the starch chain to produce glucose, which is not conducive to RS formation. The stretched H-conformation without intramolecular hydrogen bonds produced by heat treatment was not hydrolysed by α-amylase. However, it occupied the active groove and formed strong interactions with α-amylase, which prevented other starch molecules from binding to α-amylase, thus reducing hydrolysis efficiency. Moreover, the total interaction energies between the three starch molecules and α-amylase were approximately 78 kJ/mol. And several hydrogen bonds were formed between the starch molecules and α-amylase, which provides evidence for the continuous sliding hydrolysis hypothesis of α-amylase. Moreover, these results provide an important reference for elucidating the mechanism of RS formation.


Asunto(s)
Enlace de Hidrógeno , Simulación del Acoplamiento Molecular , Almidón , alfa-Amilasas , Almidón/química , Almidón/metabolismo , Hidrólisis , alfa-Amilasas/química , alfa-Amilasas/metabolismo , Simulación de Dinámica Molecular , Conformación Molecular
2.
Artículo en Chino | WPRIM (Pacífico Occidental) | ID: wpr-387640

RESUMEN

Objective To investigate the effect of induced aborion on stage of labor and placental abnormality of primiparous women during parturition. Methods Birth process and abnormal placenta during labor for 74 women with a history of induced abortion (observation group) and 102 women without a history of induced abortion ( control group) in their first parities were collected and compared. Results The third stage of labor of the observation group was (10.75 ±2.64) min,longer than (8.12 ±3.26)min of the control group,the difference had statistical significance(P <0.05). The rate of placenta adhesion,placental remnants and low lying placenta of the observation group were 13.52%, 10.81% and 8.11% respectively, significantly higher than 2.95 %, 1.96% and 0.98% in the control group(P <0. 05). Conclusion It was demonstrated that induced aborion had obvious untoward effect on pregnancy such as prolonged third stage of labor and increased incidence of placental abnormalities. So, it was necessary to prevent induced aborion for primiparous women.

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