RESUMEN

A water-soluble polysaccharide (HPS3aS) with a molecular mass of 1.22 × 10(4) Da was isolated from Hedysarum polybotrys using anion-exchange and gel-permeation chromatography. HPS3aS exhibits a globular-shaped conformation in 0.1 M NaNO3 by size exclusion chromatography with multi-angle laser light scattering (SEC-MALLS). The investigation of the structural features of this heteropolysaccharide through a combination of chemical and instrumental analyses revealed that the backbone of HPS3aS is composed of α-D-(1 → 4)-linked glucopyranose residues, which occasionally branched at O-6. The branches are composed of (1 → 4)-linked galactopyranose residues and terminated with glucopyranose residues. HPS3aS possesses good in vitro antioxidant activity, as evaluated by scavenging assays with 1,1-diphenyl-2-picrylhydrazyl, hydroxyl, and superoxide radicals, which suggests that HPS3aS could be a potential antioxidant.

Asunto(s)

Antioxidantes/aislamiento & purificación , Antioxidantes/farmacología , Medicamentos Herbarios Chinos/aislamiento & purificación , Medicamentos Herbarios Chinos/farmacología , Fabaceae/química , Depuradores de Radicales Libres/aislamiento & purificación , Depuradores de Radicales Libres/farmacología , Polisacáridos/aislamiento & purificación , Polisacáridos/farmacología , Antioxidantes/química , Compuestos de Bifenilo/farmacología , Medicamentos Herbarios Chinos/química , Depuradores de Radicales Libres/química , Estructura Molecular , Resonancia Magnética Nuclear Biomolecular , Oxidación-Reducción , Picratos/farmacología , Raíces de Plantas/química , Polisacáridos/química , Superóxidos/análisis , Superóxidos/química , Agua/química