RESUMEN
OBJECTIVE: To investigate the effects of Chaihu Shugan San (CHSGS), a compound traditional Chinese herbal medicine, on behavior and plasma levels of corticotropin releasing hormone (CRH) and adrenocorticotropic hormone (ACTH) of rats with chronic mild unpredicted stress depression. METHODS: Forty male Sprague-Dawley rats were randomly divided into 4 groups: normal control group, untreated group, fluoxetine group and CHSGS group. Except the normal control group, rats were singly housed and exposed to an unpredicted sequence of mild stressor for continuous 4 weeks to induce depression. Since the fifteenth day, rats were intragastrically administered with equal volume agents respectively for 2 weeks [normal saline for the normal control group and the untreated group, fluoxetine (1.8 mg/kg) for the fluoxetine group and CHSGS (5.9 g/kg) for the CHSGS group]. Behavioral scores of rats were detected by open-field test and sucrose preference test, and the plasma levels of CRH and ACTH in different groups were detected by radioimmunoassay. RESULTS: Compared with the normal control group, body weights of the rats in the untreated group were significantly decreased. Scores of crossing, rears and grooming in open-field test were reduced significantly. Pure water consumption in sucrose preference test was increased significantly. The levels of plasma CRH and ACTH were significantly increased. The depressive behaviors of the rats were improved significantly and the levels of plasma CRH and ACTH were obviously reduced in the CHSGS group. CONCLUSION: Chronic mild unpredicted mild stress can affect the neuroendocrine and behavior and cause depression in rats. CHSGS can regulate HPA hyperactivity of rats caused by chronic stress and has antidepressive effects.
Asunto(s)
Hormona Adrenocorticotrópica/sangre , Hormona Liberadora de Corticotropina/sangre , Depresión/tratamiento farmacológico , Fitoterapia , Extractos Vegetales/uso terapéutico , Animales , Conducta Animal/efectos de los fármacos , Depresión/sangre , Depresión/etiología , Masculino , Ratas , Ratas Sprague-Dawley , Estrés FisiológicoRESUMEN
OBJECTIVE: To study the clinical features and successful management of a survived human case with A/H5N1 infection. METHODS: The data of a confirmed case of human case with A/H5N1 infection in Hunan province were collected and analyzed. RESULTS: This patient with A/H5N1 infection in Hunan province was confirmed by reverse-transcription polymerase chain reaction (RT-PCR) for A/H5N1 in airway secretions. The main clinical presentations included fever, cough and dyspnea. An extensive pulmonary infiltration developed quickly, followed by multi-organ dysfunction. Early administration of oseltamivir, early protection of organ function and extensive support were effective for the cure of the disease in this case. CONCLUSION: Early administration of oseltamivir, early protection of organ function and adequate support therapy may be useful for the treatment of human A/H5N1 infection.
Asunto(s)
Gripe Humana/terapia , Insuficiencia Multiorgánica/terapia , Adulto , Antivirales/uso terapéutico , China , Femenino , Humanos , Subtipo H5N1 del Virus de la Influenza A , Gripe Humana/virología , Insuficiencia Multiorgánica/etiología , Oseltamivir/uso terapéutico , Resultado del TratamientoRESUMEN
AIM: To investigate the correlation between serum MMP-2 and MMP-9 in patients with acute myocardial infarction (AMI) before and after PCI. METHODS: The enzymatic activity and protein expression level of serum MMP-2 and MMP-9 in 56 patients with AMI and 20 control subjects were detected by the SDS-PAGE enzymograph and Western blot before and 1-7 days after PCI. The correlation between MMP-2 and MMP-9 was also analyzed. RESULTS: The enzymatic activity and protein expression level of serum MMP-2 and MMP-9 in 56 patients with AMI were both significantly higher than those in the control subjects before and after PCI (P<0.01). The enzymatic activity and protein expression level of serum MMP-2 and MMP-9 in patients with AMI began to increase on the first day and were at peak on the third day (P<0.01). They began to decrease on the fifth day after PCI (P<0.05). The enzymatic activity of serum MMP-2 was correlated positively with that of MMP-9 (P<0.01). The protein expression level of serum MMP-2 was also correlated positively with that of MMP-9 (P<0.01). CONCLUSION: The serum MMP-2 and MMP-9 may be related to inflammatory injuries after myocardial ischemia and reperfusion. MMP-2 can be regarded as an important marker of inflammation as MMP-9 in the pathogenesis of AMI.
Asunto(s)
Metaloproteinasa 2 de la Matriz/sangre , Metaloproteinasa 9 de la Matriz/sangre , Infarto del Miocardio/enzimología , Infarto del Miocardio/cirugía , Adulto , Anciano , Angioplastia Coronaria con Balón , Estudios de Casos y Controles , Femenino , Humanos , Masculino , Metaloproteinasa 2 de la Matriz/genética , Metaloproteinasa 9 de la Matriz/genética , Persona de Mediana Edad , Infarto del Miocardio/sangre , Infarto del Miocardio/genéticaRESUMEN
OBJECTIVE: To investigate the mechanism of glossy ganoderma decoction in Amanita mushroom poisoning. METHODS: Twenty male New Zealand white rabbits were randomly divided into 4 groups, including a normal control, a model poison group, and 2 treatment groups (different doses of glossy ganoderma decoction). The activities of hepatocyte RNA polymerase were measured by ultraviolet spectrophotometry and liver function were measured. RESULTS: The activities of hepatocyte RNA polymerase of the model group significantly decreased, and those of the 2 treatment groups were significantly higher than those of the model group. There was a dose-dependent manner between the 2 treatment groups ( all Ps<0.01), and the differences of liver function test including total bilirubin (TBIL), direct bilirubin (DB), total bile acid (TBA), and alanine aminotransferase (ALT) in the 4 groups were significant (P<0.01). CONCLUSION: Glossy ganoderma decoction may protect the liver from Amanita mushroom poisoning. Its mechanism may be related to the increase of the activities of hepatocyte RNA polymerase.
Asunto(s)
Ganoderma , Hepatocitos/efectos de los fármacos , Intoxicación por Setas/fisiopatología , Alanina Transaminasa/metabolismo , Amanita , Animales , ARN Polimerasas Dirigidas por ADN/metabolismo , Hepatocitos/metabolismo , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , Medicina Tradicional China , Intoxicación por Setas/tratamiento farmacológico , ConejosRESUMEN
OBJECTIVE: To assess the effects of treatment of Amanita mushroom poisoning with Glossy anoderma Decoction (, GGD). METHODS: Twelve patients with acute Amanita mushroom poisoning received conventional treatment (penicillin and reduced glutathione) combined with oral administration of GGD (treated group), which was prepared out of 200 g Glossy ganoderma decocted in water to 600 mL, and 200 ml was given once, three times a day for 7 successive days; while conventional treatment alone was given to the other 11 patients assigned to the control group. The therapeutic efficacy and changes in serum levels of total bilirubin (TBil), bile acids (BA), alanine transaminase (ALT), and aspartate transaminase (AST) activities in the two groups were compared. RESULTS: The cured-markedly effective rate in the treated group was more significant than that in the control group (P<0.01). Elevation in TBil, BA, ALT, and AST activities were observed in both groups 3 days after poisoning, which progressively increased thereafter in the control group. In the treated group, they reached their peak on the 3rd day and then declined gradually. The differences between pre-treatment and post-treatment in both groups were obviously significant (P<0.01), so were the differences between the two groups at corresponding time points (P<0.01). CONCLUSION: GGD shows excellent clinical efficacy in the treatment of acute Amanita mushroom poisoning and can reduce mortality significantly.
Asunto(s)
Ganoderma , Intoxicación por Setas/tratamiento farmacológico , Enfermedad Aguda , Adolescente , Adulto , Amanita , Ácidos y Sales Biliares/sangre , Niño , Femenino , Humanos , Masculino , Medicina Tradicional China , Persona de Mediana Edad , Intoxicación por Setas/sangre , Intoxicación por Setas/mortalidadRESUMEN
OBJECTIVE: To observe the effect of Ganoderma lucidum decoction in treating Russula subnigricans poisoning (RSP) patients. METHODS: The 14 patients of RSP in the treated group were treated with GLD (GLD, one dose was prepared by 100 g of Ganoderma lucidum decocted with water to 600 ml), on the base of conventional treatment, and 11 patients received conventional therapy in the previous year were taken as control. The clinical efficacy and parameters in them were compared, including the urine N-acetyl-D-glucosaminidase (NAG, which reflects the injury of kidney), the red blood cell and protein in urine, the alanine transaminase (ALT, which reflects the injury of liver), and the aspartate aminotransferase (AST, which reflects the injury of heart). RESULTS: A better clinical cure-markedly improving rate was showed in the treated group as compared with the control group, P < 0.01. In the treated group, red blood cell in urine disappeared after 24 hrs treatment in the majority of patients, urinary protein reduced obviously and the other three parameters reached the peak at the 3rd day then lowered gradually. In the control group, all the parameters increased continuously. Comparison between the parameters at corresponding time in the two groups showed significant difference (P < 0.01), those in the treated group were markedly lower than those in the control group respectively. CONCLUSION: GLD has good effect in treating RSP, could obviously lower the fatat rate of RSP.