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This study investigated the effect of α-adrenoceptor agonists microinjected into the shell region of the accumbens nucleus (AcbSh) on feeding and anxiety-related behaviors in free-feeding rats. Male Wistar rats with a chronically implanted cannula into the AcbSh were unilaterally microinjected with either clonidine (CLON, α(2)-adrenoceptor agonist) or phenylephrine (PHEN, α(1)-adrenoceptor agonist) at the doses of 6 and 20 nmol and submitted to the elevated plus-maze (EPM), a pre-clinical test of anxiety. Immediately after the EPM test, the animals underwent food intake evaluation for 30 min. The data showed that rats microinjected with CLON (20 nmol/0.2 µl) into the AcbSh exhibited increased %Open arm time, which is compatible with an anxiolytic-like effect. The CLON-induced anxiolysis was corroborated by increased head-dipping and decreased stretched-attend posture, two ethologically derived behaviors which are fear/anxiety-motivated. The animal's locomotor activity was not changed by 20 nmol CLON microinjection into the AcbSh. However, neither dose of PHEN microinjected into the AcbSh was able to alter either the spatial-temporal or ethological variables representative of fear/anxiety and locomotion. Food intake was not altered by any dose of CLON and PHEN microinjected into the AcbSh, but the 20 nmol CLON microinjection induced increased motor activity in the feeding test. The data suggests that noradrenergic projections to the AcbSh may underlie fear/anxiety modulation through α(2)-adrenoceptor in the AcbSh, while feeding behavior was unaffected by noradrenergic modulation in the AcbSh of free-feeding rats. This article is part of a Special Issue entitled 'Anxiety and Depression'.

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Introdução: O uso combinado do curativo com pressão negativa (CPN)com a matriz de regeneração dérmica (MRD) tem melhorado os resultadosdo tratamento de feridas extensas e simplificado o seguimento desses pacientes. Nós analisamos os resultados de pacientes que receberam MRD e comparamos com aqueles que receberam MRD associado com CPN.Método: Trata-se de uma pesquisa clínico-epidemiológica, observacional,com coleta de dados retrospectiva, que avaliou os prontuários de todas as crianças submetidas a aplicação de MRD, com ou sem CPN como adjuvante,no período de janeiro de 2002 a março de 2010, totalizando 66 pacientes. Resultados: As principais complicações após aplicação de MRD sem CPN são o hematoma (26,79%) e a infecção (17,86%), resultando em uma taxa média de pega da matriz de 88,81%. A principal complicação após o implante de MRD associado ao CPN foi o hematoma (22,22%), e a taxa média depega da DRT foi 90,56%. O tempo de maturação da MRD associado ao CPN foi 15,88 dias. O resultado final foi o enxerto de pele em 100% dos casos, com uma taxa de pega de 93,62%. Conclusões: O CPN oferece vantagens como adjuvante no tratamento de feridas com MRD. Reduz o tempo de maturação da MRD, diminui o tempo de hospitalização, reduz as complicações após implante e promove aumento da taxa de pega da matriz.

Background: The use of negative pressure wound therapy (NPWT) dressings with dermal regeneration template (DRT) has improved outcomes and simplified aftercare in patients with extensive wounds. We analyzed our results with DRT associated with the NPWT also without NPWT. Methods: The medical files of all children submitted to DRT application with NPWT also without NPWT as adjuvant, from January 2002 to March 2010, were accessed, in atotal of 66 patients. Results: The main complications after DRT without NPWT application were haematomas (26.79%) and infections (17.86%), resulting in a mean take rate of DRT of 88.81%. The main early complication after DRT implantation and use of NPWT was the hematoma (22.22%), and the mean take rate of the DRT was 90.56%. On average, the maturation time of DRT using the NPWT was 15.88 days. The final outcome was skin grafting in 100% of cases. The epidermal graft achieved the average take rate of 93.62%. Conclusions: The NPWT offers advantages in the adjuvant treatment of DRT, as reduction of the maturation time of DRT, shorter hospitalization, reduction of complication after DRTimplantation and improve take rate of DRT.

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