RESUMEN

Biochemical assays that can identify ß-lactamase activity directly from patient samples have the potential to significantly improve the treatment of bacterial infections. However, current ß-lactamase probes do not have the sensitivity needed to measure ß-lactam resistance directly from patient samples. Here, we report the development of an instrument-free signal amplification technology, DETECT, that connects the activity of two enzymes in series to effectively amplify the activity of ß-lactamase 40 000-fold, compared to the standard ß-lactamase probe nitrocefin.

Asunto(s)

Infecciones Bacterianas/diagnóstico , Infecciones Bacterianas/microbiología , beta-Lactamasas/orina , Cefalosporinas/química , Humanos , Límite de Detección , Resistencia betalactámica