RESUMEN

Erwinia carotovora subsp. carotovora CSDS001 elicits hypersensitive reaction (HR) in tobacco. From the genomic libraries of Erwinia carotovora subsp. carotovora CSDS001, the hrpNCSDS001 gene (GenBank number AY939927), was isolated. The hrpNCSDS001 fusion protein was produced in Escherichia coli, and was used to induce HR by injecting into tobacco. We further examined the global regulation of Arabidopsis thaliana genes in response to HarpinCSDS001 at a concentration of 30 miccrog/mL. We indicated that 912, 1787, 2393, 1833 and 1,755 genes that were regulated significantly (log ratio or=1) at 3 h, 12 h, 24 h, 36 h and 48 h respectively after the treatment. Analysis of some transcription factors (TF) showed that 13 TF families responded to HarpinCSDS001 including ZIM, BES1, TCP, C2C2, AP2/EREBP, WRKY, bHLH, bZIP, GARP, MYB, NAC, HB, C2H2. These families mainly function in biological processes of plant defense, pho-tosynthesisdevelopment and flowering.

Asunto(s)

Arabidopsis/efectos de los fármacos , Arabidopsis/genética , Proteínas de la Membrana Bacteriana Externa/farmacología , Perfilación de la Expresión Génica , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Pectobacterium carotovorum/metabolismo , Secuencia de Aminoácidos , Arabidopsis/crecimiento & desarrollo , Arabidopsis/inmunología , Proteínas de la Membrana Bacteriana Externa/química , Proteínas de la Membrana Bacteriana Externa/aislamiento & purificación , Proteínas de la Membrana Bacteriana Externa/metabolismo , Clonación Molecular , Escherichia coli/genética , Cinética , Datos de Secuencia Molecular , Análisis de Secuencia por Matrices de Oligonucleótidos , Pectobacterium carotovorum/patogenicidad , Análisis de Secuencia de ADN