RESUMEN
Given raised rates of patient suicide and violence in secure psychiatric facilities, staff in such settings are arguably at increased risk for burnout and reduced mental health. The present article responds to the recent U.K. National Institute for Health and Care Excellence (NICE) call to assess workforce well-being. This article held the following aims: (1) to quantify existing levels of mental health (i.e., depression, anxiety, distress, and posttraumatic stress) and subjective well-being (i.e., job satisfaction, life satisfaction, and four domains of burnout) and (2) to evaluate Coping Self-Efficacy (CSE) and Need for Affect (NFA) as factors associated with staff mental health and subjective well-being. We conducted a voluntary cross-sectional health needs assessment of forensic mental health staff (N = 170) between 2017 and 2018 from one National Health Service (NHS) Trust. Descriptive findings suggest staff possessed nonclinical average ranges of mental health symptoms. Subjective well-being findings showed burnout was relatively low, whereas job and life satisfaction were modest. Regression models demonstrated that (a) thought/emotion stopping beliefs were negatively associated with psychological exhaustion; (b) social support beliefs were positively associated with life satisfaction and job enthusiasm; (c) NFA Avoidance was linked with poor mental health and burnout, and; (d) NFA Approach was positively associated with two health subjective well-being indicators. Overall, assessment results suggest NHS forensic mental health staff reported relatively good health. Cognitive- and emotion-focused coping beliefs demonstrate promise as content for prevention programming. Using Emotional Labor Theory, we offer psychological services-based recommendations for future prevention programming and research. (PsycInfo Database Record (c) 2020 APA, all rights reserved).
Asunto(s)
Adaptación Psicológica , Síntomas Conductuales/psicología , Agotamiento Profesional/psicología , Psiquiatría Forense , Hospitales Psiquiátricos , Satisfacción en el Trabajo , Satisfacción Personal , Personal de Hospital/psicología , Autoeficacia , Apoyo Social , Trastornos por Estrés Postraumático/psicología , Adulto , Síntomas Conductuales/epidemiología , Agotamiento Profesional/epidemiología , Estudios Transversales , Femenino , Psiquiatría Forense/estadística & datos numéricos , Hospitales Psiquiátricos/estadística & datos numéricos , Humanos , Masculino , Persona de Mediana Edad , Programas Nacionales de Salud , Personal de Hospital/estadística & datos numéricos , Trastornos por Estrés Postraumático/epidemiología , Reino Unido/epidemiologíaRESUMEN
Various pathogenic clostridia produce binary protein toxins associated with enteric diseases of humans and animals. Separate binding/translocation (B) components bind to a protein receptor on the cell surface, assemble with enzymatic (A) component(s), and mediate endocytosis of the toxin complex. Ultimately there is translocation of A component(s) from acidified endosomes into the cytosol, leading to destruction of the actin cytoskeleton. Our results revealed that CD44, a multifunctional surface protein of mammalian cells, facilitates intoxication by the iota family of clostridial binary toxins. Specific antibody against CD44 inhibited cytotoxicity of the prototypical Clostridium perfringens iota toxin. Versus CD44(+) melanoma cells, those lacking CD44 bound less toxin and were dose-dependently resistant to C. perfringens iota, as well as Clostridium difficile and Clostridium spiroforme iota-like, toxins. Purified CD44 specifically interacted in vitro with iota and iota-like, but not related Clostridium botulinum C2, toxins. Furthermore, CD44 knockout mice were resistant to iota toxin lethality. Collective data reveal an important role for CD44 during intoxication by a family of clostridial binary toxins.
Asunto(s)
ADP Ribosa Transferasas/toxicidad , Toxinas Bacterianas/toxicidad , Endocitosis/fisiología , Receptores de Hialuranos/metabolismo , Animales , Western Blotting , Línea Celular Tumoral , Chlorocebus aethiops , Ditiotreitol/farmacología , Relación Dosis-Respuesta a Droga , Receptores de Hialuranos/genética , Inmunoprecipitación , Ratones , Ratones Noqueados , Células VeroRESUMEN
Clostridium difficile is a major enteropathogen of humans. It produces two main virulence factors, toxins A and B. A third, less well known toxin, C. difficile toxin (CDT), is a binary toxin composed of distinct enzymatic (CdtA) and cell binding/translocation (CdtB) proteins. We used a novel enzyme linked immunoassay (EIA) to detect CdtB protein in feces and culture fluids. Additionally, PCR was used to assay C. difficile isolates from fecal samples for the CDT locus (CdtLoc). Although the results from 80 isolates suggest no relationship between toxin concentrations in situ and in vitro, there is a good correlation between PCR detection of the cdtB gene and EIA detection of CdtB protein in vitro. Possible implications of the detection of CDT in patients are discussed.
Asunto(s)
ADP Ribosa Transferasas/análisis , Proteínas Bacterianas/análisis , Toxinas Bacterianas/análisis , Clostridioides difficile/metabolismo , Diarrea/microbiología , ADP Ribosa Transferasas/genética , Proteínas Bacterianas/genética , Toxinas Bacterianas/genética , Clostridioides difficile/genética , Clostridioides difficile/aislamiento & purificación , Heces/microbiología , Humanos , Técnicas para InmunoenzimasRESUMEN
We investigated the frequency of Clostridium perfringens in the normal fecal flora of healthy North Americans. About half of 43 subjects were colonized with C. perfringens at levels of approximately 10(6)cfu/g feces. Only type A strains were recovered. Spores sometimes outnumbered vegetative cells. Several genotypes were found. Some donors carried two genotypes, some only one. We found no alpha, beta2 or enterotoxin in the stools of any donors. Though some isolates carried toxin genes (e.g. cpe and cpb2) on plasmids, we saw no indication that healthy humans are the reservoir for the chromosomally-borne cpe recovered from cases of C. perfringens food poisoning.
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Toxinas Bacterianas/genética , Clostridium perfringens/genética , Proteínas de Unión al Calcio/genética , Portador Sano/microbiología , Clostridium perfringens/aislamiento & purificación , Recuento de Colonia Microbiana , Enterotoxinas/genética , Heces/microbiología , Femenino , Genotipo , Humanos , Masculino , América del Norte , Plásmidos , Esporas Bacterianas/aislamiento & purificación , Fosfolipasas de Tipo C/genéticaRESUMEN
Several different nomenclatures have been applied to the Clostridium difficile toxins and their associated genes. This paper summarizes the new nomenclature that has been agreed to by the research groups currently active in the field. The revised nomenclature includes C. difficile toxins and other related large clostridial toxins produced by Clostridium sordellii and Clostridium novyi, and corresponding toxin genes, as well as toxin production types of C. difficile strains.
Asunto(s)
Toxinas Bacterianas/genética , Clostridioides difficile/genética , Terminología como Asunto , Proteínas Bacterianas/genética , Toxinas Bacterianas/aislamiento & purificación , Enterotoxinas/genéticaRESUMEN
RicinB, the non-toxic galactose/N-acetylgalactosamine-binding subunit of ricin, was fused to a model antigen, green fluorescent protein (GFP), and expressed in tobacco plants and hairy root cultures to test for utility in mucosal vaccine delivery/adjuvancy. The fusion protein retained both GFP fluorescence and galactose/galactosamine-binding activity. Intranasal immunization of mice with galactosamine-affinity purified ricinB:GFP recovered from tobacco root cultures triggered significant increases in GFP-specific serum IgGs. This strong humoral response was comparable to that observed following GFP immunization with cholera toxin adjuvant. GFP at the same concentrations but without an adjuvant was non-immunogenic. Induction of higher levels of IgG(1) than IgG(2a) following ricinB:GFP immunization suggested the presence of a Th2 response. Serum and fecal anti-GFP IgA were also induced by immunization with ricinB:GFP. Our data suggest that ricinB can be used as an adjuvant and antigen carrier to the mucosa and is efficient in eliciting systemic and mucosal immune responses.
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Adyuvantes Inmunológicos/administración & dosificación , Ricina/administración & dosificación , Vacunas/administración & dosificación , Administración Intranasal , Animales , Formación de Anticuerpos , Antígenos/administración & dosificación , Femenino , Proteínas Fluorescentes Verdes , Inmunidad Mucosa , Proteínas Luminiscentes/administración & dosificación , Proteínas Luminiscentes/genética , Proteínas Luminiscentes/inmunología , Ratones , Ratones Endogámicos ICR , Plantas Modificadas Genéticamente , Proteínas Recombinantes/administración & dosificación , Proteínas Recombinantes/genética , Proteínas Recombinantes/inmunología , Ricina/genética , Nicotiana/genéticaRESUMEN
Amebic colitis is an important worldwide parasitic disease for which there is not a well-established animal model. In this work we show that intracecal inoculation of Entamoeba histolytica trophozoites led to established infection in 60% of C3H mice, while C57BL/6 or BALB/c mice were resistant, including mice genetically deficient for IL-12, IFN-gamma, or inducible NO synthase. Infection was a chronic and nonhealing cecitis that pathologically mirrored human disease. Characterization of the inflammation by gene chip analysis revealed abundant mast cell activity. Parasite-specific Ab and cellular proliferative responses were robust and marked by IL-4 and IL-13 production. Depletion of CD4(+) cells significantly diminished both parasite burden and inflammation and correlated with decreased IL-4 and IL-13 production and loss of mast cell infiltration. This model reveals important immune factors that influence susceptibility to infection and demonstrates for the first time the pathologic contribution of the host immune response in amebiasis.
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Linfocitos T CD4-Positivos/inmunología , Linfocitos T CD4-Positivos/parasitología , Modelos Animales de Enfermedad , Disentería Amebiana/inmunología , Disentería Amebiana/parasitología , Animales , Anticuerpos Antiprotozoarios/biosíntesis , Linfocitos T CD4-Positivos/metabolismo , Ciego/inmunología , Ciego/parasitología , Ciego/patología , División Celular/inmunología , Enfermedad Crónica , Cricetinae , Susceptibilidad a Enfermedades/inmunología , Disentería Amebiana/patología , Disentería Amebiana/prevención & control , Entamoeba histolytica/crecimiento & desarrollo , Entamoeba histolytica/inmunología , Femenino , Inflamación/inmunología , Inflamación/parasitología , Mucosa Intestinal/inmunología , Mucosa Intestinal/parasitología , Mucosa Intestinal/patología , Depleción Linfocítica , Ratones , Ratones Endogámicos BALB C , Ratones Endogámicos C3H , Ratones Endogámicos C57BL , Ratones Noqueados , Índice de Severidad de la Enfermedad , Especificidad de la EspecieRESUMEN
TxeR, a sigma factor that directs Clostridium difficile RNA polymerase to recognize the promoters of two major toxin genes, was shown to stimulate its own synthesis. Whether expressed in C. difficile, Clostridium perfringens, or Escherichia coli, TxeR stimulated transcription of fusions of the txeR promoter region to reporter genes. As is the case for the tox genes, txeR expression was responsive to the cellular growth phase and the constituents of the medium. That is, the level of expression in broth culture was low during the exponential growth phase, but rapidly increased as cells approached the stationary phase. In the presence of excess glucose, expression from the txeR promoter was repressed. The results support a model for toxin gene expression in which synthesis of TxeR is induced by specific environmental signals. The increased level of TxeR then permits high-level expression of the toxin genes. The study of txeR gene regulation in C. difficile was made possible by introduction of a mobilizable, replicative plasmid via conjugation with E. coli.