RESUMEN
OBJECTIVE: The oxidative burst of the host cells associated with bacterial pathogen infection contributes to the destruction of periodontal tissue. The present study investigates the effect of docosahexaenoic acid (DHA) on human gingival fibroblast (HGF) viability and ROS generation. METHODS: The cell viability by MTT assay, ROS level using H2DCF-DA probe, and protein thiol content were measured in HGFs after 24h preincubation with different concentrations of DHA followed by treatment with H2O2. The cell death rate was determined by Annexin V/propidium iodide staining, and mitochondrial membrane potential (ΔΨm) was examined by MitoTracker Red probe in H2O2- and butyric acid-treated HGFs. The fatty acid composition of plasma membranes after incubation with DHA was determined by gas chromatography mass spectrometry. RESULTS: DHA preincubation in a dose-dependent manner increased the viability of HGFs exposed to H2O2 and decreased ROS generation compared to the control cells. In HGFs preincubated with 30µM DHA, the ΔΨm significantly increased in both H2O2- and butyric acid-treated cells. Moreover, incubation with DHA preserved the protein thiol level as effectively as N-acetylcysteine. Application of 50µM DHA increased the quantity of viable cells, decreased the number of necrotic cells after H2O2 treatment, and protected HGFs from apoptosis induced by butyric acid. DHA in the plasma membranes of these HGFs represented about 6% of the total amount of fatty acids. CONCLUSIONS: These results demonstrate that enrichment of HGFs with DHA reduces ROS generation and enhances the mitochondrial membrane potential protecting the fibroblasts against cytotoxic factors.
Asunto(s)
Ácidos Docosahexaenoicos/farmacología , Fibroblastos/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Apoptosis/efectos de los fármacos , Ácido Butírico/toxicidad , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Ácidos Grasos/metabolismo , Cromatografía de Gases y Espectrometría de Masas , Humanos , Peróxido de Hidrógeno/toxicidad , Potencial de la Membrana Mitocondrial/efectos de los fármacos , Necrosis , Especies Reactivas de Oxígeno/metabolismoRESUMEN
UNLABELLED: The aim of the study was to estimate the periodontal state in chronic obstructive pulmonary disease (COPD). MATERIAL AND METHODS: The study comprised 100 patients with COPD (68 men and 32 women), aged 30-83 years (mean 63.1 +/- 10.17 years). All patients were in the II grade COPD (moderate COPD). FEV1/FVC<70% and FEV1 was within 79% and 50% of the predicted value. Beta2-adrenomimetic inhalants or in nebulization, theophylline were applied in the therapy. The control group consisted of 101 subjects (38 men and 63 women), aged 42-88 years (mean 65.3-10.36 years), with no respiratory tract diseases. The rate of smoking was similar in both groups. To examine periodontal state, periodontal pocket depth was measured (with periodontal sound), Periodontal Index (PI) and dental plaque index were assessed. RESULTS: The frequency and severity of periodontal diseases in COPD patients were demonstrated to be significantly increased as compared to the control group (p<0.05). Adult chronic periodontitis (ACP) was observed significantly more frequently in COPD patients (84.21%) than in the control group (59.74%, p<0.05). A positive correlation was found between periodontal pocket depth and dental plaque index in patients with COPD. CONCLUSION: The frequency and intensification of periodontal diseases speak for their cause-and-effect relationship with COPD. The prophylaxis and treatment of periodontal diseases should be of great importance in the COPD therapy, which may increase its effectiveness.
Asunto(s)
Periodontitis/epidemiología , Enfermedad Pulmonar Obstructiva Crónica/epidemiología , Adulto , Anciano , Anciano de 80 o más Años , Estudios de Casos y Controles , Comorbilidad , Femenino , Educación en Salud , Humanos , Masculino , Persona de Mediana Edad , Salud Bucal , Periodontitis/prevención & control , Polonia/epidemiología , Factores de Riesgo , Encuestas y CuestionariosRESUMEN
In the last few years the interest of extraesophageal symptoms of gastroesophageal reflux disease is getting greater and greater. Because of it is often appearance, this disease is noticed not only by gastroenterologists, but also other doctors and dentists. They think that pathological, backward reflux of esophagus in many cases can be responsible for dental organ disease. Clinical research proofs that often pouring the sour mixture from the stomach into the esophagus and then to mouth can cause a diminution in the teeth such as: erosion and decay. The background agum and tounque can be gastroesophageal reflux disease. acquantance with this facts among the dentists can help to make a diagnosis and treat well patients with a pathological reflux of esophagus.