RESUMEN
Current radiocesium (137Cs) models to evaluate the risk of 137Cs transfer from soil to plants are based on the clay and exchangeable potassium (K) contents in soil. These models disregard the mineralogy of the clay fraction and are likely not capable of accurately predicting the 137Cs transfer factor (TF) in soils of contrasting parent rocks and weathering stages. The objectives of this study were to test that hypothesis and to identify whether quantitative information on mineralogy can improve the predictions. A pot cultivation experiment was set up with clay-sand mixtures in single and double clay doses that were fertilized, spiked with 137Cs and grown with ryegrass for 30 days. Four clays (illite, biotite, smectite and vermiculite) along with six deposits from clay-rich geological units were compared. The TF generally decreased with increasing clay dose for each of these ten different clay groups, however, the TF varied two orders of magnitude across clay groups and doses. The TF was highest for clays with little 137Cs specific sites such as bentonite and/or where the exchangeable K content was low compared to the other clays. The TF was well predicted from the soil solution 137Cs and K concentrations (R2 = 0.72 for log transformed TF), corroborating earlier findings in natural soils. The TF (log transformed) was statistically unrelated to total phyllosilicate content or 1:1 and 2:1:1 type phyllosilicate content while it significantly decreased with increasing 2:1 phyllosilicate content (R2 = 0.32). A multiple regression model with four different X-ray diffraction (XRD) based phyllosilicate groups yielded the strongest predictive power (R2 = 0.74). We conclude that XRD quantification is valuable for describing 137Cs bioavailability in plant substrates. These findings now await confirmation for natural soils.
Asunto(s)
Radioisótopos de Cesio , Arcilla , Lolium , Contaminantes Radiactivos del Suelo , Disponibilidad Biológica , Radioisótopos de Cesio/análisis , Arcilla/química , Plantas , Suelo/química , Contaminantes Radiactivos del Suelo/análisisRESUMEN
The zyxin family of proteins consists of five members, ajuba, LIMD1, LPP, TRIP6 and zyxin, which localize at cell adhesion sites and shuttle to the nucleus. Previously, we established that LPP interacts with the tumor suppressor Scrib, a member of the leucine-rich repeat and PDZ (LAP) family of proteins. Here, we demonstrate that Scrib also interacts with TRIP6, but not with zyxin, ajuba, or LIMD1. We show that TRIP6 directly binds to the third PDZ domain of Scrib via its carboxy-terminus. Both proteins localize in cell-cell contacts but are not responsible to target each other to these structures. In the course of our experiments, we also characterized the nuclear export signal of human TRIP6, and show that LIMD1 is localized in focal adhesions. The binding between two of zyxin's family members and Scrib links Scrib to a communication pathway between cell-cell contacts and the nucleus, and implicates these zyxin family members in Scrib-associated functions.
Asunto(s)
Glicoproteínas/metabolismo , Proteínas de la Membrana/metabolismo , ATPasas Asociadas con Actividades Celulares Diversas , Proteínas Adaptadoras Transductoras de Señales/genética , Proteínas Adaptadoras Transductoras de Señales/metabolismo , Secuencia de Aminoácidos , Animales , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Línea Celular , Proteínas del Citoesqueleto/genética , Proteínas del Citoesqueleto/metabolismo , Adhesiones Focales/metabolismo , Glicoproteínas/clasificación , Glicoproteínas/genética , Proteínas de Homeodominio/genética , Proteínas de Homeodominio/metabolismo , Humanos , Péptidos y Proteínas de Señalización Intracelular , Proteínas con Dominio LIM , Proteínas de la Membrana/genética , Datos de Secuencia Molecular , Filogenia , Complejo de la Endopetidasa Proteasomal , Unión Proteica , Estructura Terciaria de Proteína , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Alineación de Secuencia , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , Proteínas Supresoras de Tumor/genética , Proteínas Supresoras de Tumor/metabolismo , Técnicas del Sistema de Dos Híbridos , ZixinaRESUMEN
For the currently used (99m)Tc-labeled diphosphonates such as (99m)Tc-MDP and (99m)Tc-HDP, the required interval of 2.5 to 3 h between injection and the scintigraphic bone imaging is an inconvenience. The present study was set up in an attempt to develop a technetium-99m-labeled diphosphonate with efficient bone uptake and more rapid clearance from blood and soft tissue by renal extraction and excretion so that it would be possible to start imaging as early as 1 h after injection. A conjugate of the new renal tracer agent (99m)Tc-ethylene dicysteine ((99m)Tc-L,L-EC), covalently bound via one of its carboxylates with aminomethylenediphosphonic acid (AMDP), was synthesized in seven steps. EC-AMDP could be labeled easily and efficiently with (99m)Tc at pH > or = 12 and room temperature. Analysis using ion pair reversed phase high performance liquid chromatography showed the formation of a mixture of two main compounds with reproducible relative ratios, which were stable as a function of time. In a baboon, the scintigraphic images obtained with the new agent showed good quality bone scans, with clear visualization of the skeleton and low soft tissue activity at respectively 1 and 2 h after injection.