RESUMEN

The influence of pH and temperature upon the dealkylation (aging) of soman-inhibited acetylcholinesterase (acetylcholine acetylhydrolase, EC 3.1.1.7) was investigated. The pH-rate profile, as measured at 5 degrees C, shows a maximum aging rate at pH 5.0 and indicates the involvement of two ionizable groups with pKa values of 6.0 and 4.5. The pH-rate profile was not changed essentially by the two effector compounds, semicarbazide and HH54. the activation energy of aging at pH 7.0 was determined as to 6.12 x 10(4) J/mol; it was lowered by the accelerator of aging semicarbazide to 5.28 x 10(4) J/mol and increased to 9.42 x 10(4) J/mol by the retarding compound HH54.

Asunto(s)

Inhibidores de la Colinesterasa , Compuestos Organofosforados/farmacología , Soman/farmacología , Remoción de Radical Alquila , Concentración de Iones de Hidrógeno , Técnicas In Vitro , Cinética , Compuestos de Metilurea/farmacología , Semicarbacidas/farmacología , Temperatura , Termodinámica