RESUMEN

Methanotrophs were enriched and isolated from polluted environments in Canada and Germany. Enrichments in low copper media were designed to specifically encourage growth of soluble methane monooxygenase (sMMO) containing organisms. The 10 isolates were characterized physiologically and genetically with one type I and nine type II methanotrophs being identified. Three key genes: 16S rRNA; pmoA and mmoX, encoding for the particulate and soluble methane monooxygenases respectively, were cloned from the isolates and sequenced. Phylogenetic analysis of these sequences identified strains, which were closely related to Methylococcus capsulatus, Methylocystis sp., Methylosinus sporium and Methylosinus trichosporium. Diversity of sMMO-containing methanotrophs detected in this and previous studies was rather narrow, both genetically and physiologically, suggesting possible constraints on genetic diversity of sMMO due to essential conservation of enzyme function.

Asunto(s)

Microbiología Ambiental , Contaminación Ambiental , Variación Genética , Methylococcus/clasificación , Methylocystaceae/clasificación , Oxigenasas/genética , Cobre/metabolismo , Medios de Cultivo , ADN Ribosómico/análisis , Genes de ARNr , Methylococcus/enzimología , Methylococcus/genética , Methylococcus/aislamiento & purificación , Methylocystaceae/enzimología , Methylocystaceae/genética , Methylocystaceae/aislamiento & purificación , Datos de Secuencia Molecular , Filogenia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Solubilidad