RESUMEN
Female BALB/cAnNCrl (n = 170; age, 6 to 9 wk) mice were infected by intravenous inoculation of 5 × 10(6) cfu Mycobacterium tuberculosis strain Erdman (ATCC 35801). Between day 52 and 5 mo after infection, 10 of the 170 mice infected according to this protocol developed torticollis, including mice in treatment groups that received combination antibiotic therapy of rifampin-pyrazinamide or moxifloxacin-rifampin-pyrazinamide. Torticollis did not develop in mice receiving isoniazid- rifampin-pyrazinamide therapy, nor was it present in the cohort of aerogenically infected mice. Affected mice were euthanized, and complete necropsy evaluation was performed on 4 mice. Gross necropsy evaluation revealed typical tuberculosis lesions in lungs of infected mice. Histologic evaluation of tissues revealed granulomatous otitis media with intralesional acid-fast bacilli consistent with Mycobacterium tuberculosis. These cases represent an unusual finding specific to the intravenous mouse model of Mycobacterium tuberculosis and may represent a model of a similar condition in humans that is known as tuberculous otitis media.
Asunto(s)
Modelos Animales de Enfermedad , Ratones , Mycobacterium tuberculosis/fisiología , Otitis Media/etiología , Tortícolis/etiología , Tuberculosis/complicaciones , Tuberculosis/tratamiento farmacológico , Animales , Antituberculosos/administración & dosificación , Antituberculosos/efectos adversos , Autopsia/veterinaria , Compuestos Aza/administración & dosificación , Compuestos Aza/efectos adversos , Quimioterapia Combinada , Oído Medio/microbiología , Oído Medio/patología , Femenino , Fluoroquinolonas , Inyecciones Intravenosas , Isoniazida/administración & dosificación , Ratones Endogámicos BALB C , Moxifloxacino , Otitis Media/microbiología , Otitis Media/patología , Pirazinamida/administración & dosificación , Pirazinamida/efectos adversos , Quinolinas/administración & dosificación , Quinolinas/efectos adversos , Rifampin/administración & dosificación , Rifampin/efectos adversos , Tortícolis/microbiología , Tortícolis/patología , Tuberculosis/patologíaRESUMEN
A wildlife sanctuary presented an adult female cottontail rabbit (Sylvilagus spp.), age unknown, to the Colorado State University Pathology service for postmortem examination. Gross examination revealed numerous pigmented wartlike lesions arising from the skin of the head surrounding the ears, eyes, nares, mouth, and dorsum. Masses were firm, friable, and easily detached from the underlying skin. Differential diagnoses included Cottontail rabbit papillomavirus, Rabbit fibroma virus, and Myxoma virus. Histological examination revealed multiple papillary masses lined by stratified squamous epithelial cells with central cores of fibrovascular connective tissue and parakeratotic hyperkeratosis. Cells of the Stratum spinosum were frequently swollen with abundant perinuclear, cytoplasmic, clearing, and occasional intranuclear basophilic, glassy, spherical inclusions up to 3 microm in diameter. The lesions were consistent with Cottontail rabbit papillomavirus infection. Papilloma virus antigens were identified by immunohistochemistry. In addition, papillomavirus particles were identified by transmission electron microscopy within Langerhans cells of the epidermis, suggesting a unique mechanism for systemic dissemination of the virus. The present case report highlights the finding of viral particles within the Langerhans cells and suggests a novel mechanism of pathogenesis.