RESUMEN
The involvement of CD4+ and CD8+ T cells in pathogenesis of spontaneous autoimmune thyroiditis (SAT) in obese strain (OS) chickens has not been studied in depth until now. We depleted CD4+ or CD8+ T cells in OS chickens by treatment with murine monoclonal anti-CD4 or anti-CD8 antibodies at 3 day intervals beginning at hatching. The birds were killed at 19-25 days of age. Treatment with anti-CD4 antibody completely prevented SAT development, while treatment with anti-CD8 antibody partially inhibited SAT. These results show the critical role of CD4+ T cells in the development of SAT in OS chickens, and indicate that CD8+ T cells are also involved in SAT pathogenesis.
Asunto(s)
Anticuerpos Monoclonales/uso terapéutico , Antígenos CD4/inmunología , Linfocitos T CD4-Positivos/inmunología , Antígenos CD8/inmunología , Linfocitos T CD8-positivos/inmunología , Pollos/genética , Modelos Animales de Enfermedad , Obesidad/veterinaria , Enfermedades de las Aves de Corral/prevención & control , Tiroiditis Autoinmune/veterinaria , Factores de Edad , Animales , Anticuerpos Monoclonales/inmunología , Autoanticuerpos/análisis , Pollos/inmunología , Técnicas para Inmunoenzimas , Endogamia , Ratones , Obesidad/genética , Enfermedades de las Aves de Corral/genética , Enfermedades de las Aves de Corral/inmunología , Enfermedades de las Aves de Corral/patología , Tiroglobulina/inmunología , Glándula Tiroides/inmunología , Glándula Tiroides/patología , Tiroiditis Autoinmune/genética , Tiroiditis Autoinmune/inmunología , Tiroiditis Autoinmune/patología , Tiroiditis Autoinmune/prevención & controlRESUMEN
Untreated control OS chickens develop spontaneous autoimmune thyroiditis (SAT). In contrast, OS chickens treated with a monoclonal anti-CD4 antibody failed to develop SAT. The preventive effect of anti-CD4 antibody on SAT was associated with the marked depletion of CD4+ T-cells by anti-CD4 treatment. These results indicate that CD4+ T-cells play a crucial role in SAT of OS chickens.