RESUMEN
Accidental release of radiocaesium (137Cs) from nuclear power plants may result in long-term contamination of environmental and food production systems. Assessment of food chain contamination with 137Cs relies on 137Cs soil-to-plant transfer data and models mainly available for regions affected by the Chornobyl and Fukushima accidents. Similar data and models are lacking for other regions. Such information is needed given the global expansion of nuclear energy. We collected 38 soils worldwide of contrasting parent materials and weathering stages. The soils were spiked with 137Cs and sown with ryegrass in greenhouse conditions. The 137Cs grass-soil concentration ratio varied four orders of magnitude among soils. It was highest in Ferralsols due to the low 137Cs interception potential of kaolinite clay and the low exchangeable potassium in these soils. Our results demonstrate, for the first time, the high plant uptake of 137Cs in tropical soils. The most recent 137Cs transfer model, mainly calibrated to temperate soils dominated by weathered micas, poorly predicts the underlying processes in tropical soils but, due to compensatory effect, still reasonably well predicts 137Cs bioavailability across all soils (R2 = 0.8 on a log-log scale).
Asunto(s)
Radioisótopos de Cesio , Monitoreo de Radiación , Contaminantes Radiactivos del Suelo , Suelo , Radioisótopos de Cesio/análisis , Contaminantes Radiactivos del Suelo/análisis , Suelo/química , Liberación de Radiactividad Peligrosa , LoliumRESUMEN
The mutants Atnoa1 and Atnia1nia2noa1-2 having a defective chloroplast developmental process, showed enhanced chlorophyll levels when they were grown on Murashige and Skoog (MS) medium and on exposure with uranium (U) on Hoagland medium. Thus we hypothesized that these mutants probably produced NO in MS medium and on exposure with U. Wild-type Col-0, Atnoa1, Atnia1nia2noa1-2 plants were cultured on modified Hoagland and 1/10 MS media and NO generation in the roots of these mutants was monitored using NO selective fluorescent dyes, DAF-2DA and Fl2E. Both Atnoa1 and Atnia1nia2noa1-2 triple mutants produced NO as observed by increases in DAF-2T and Fl2E fluorescence when these mutants were grown on MS medium but not on Hoagland medium. In presence of NO scavenger, methylene blue (MB, 200⯵M), DAF-2T and Fl2E fluorescence was completely abolished. On the other hand treatment of the plants with 25⯵M U triggered NO generation. U-treated Atnoa1 and Atnia1nia2noa1-2 plants upregulated genes (POR B, POR D, CHL D) involved in the chlorophyll biosynthesis. From these results it was concluded that Atnoa1 and Atnia1nia2noa1-2 are conditional NO producers and it appears that NO generation in plants substantially depends on growth medium and NIA1, NIA2 or NOA1 does not appear to be really involved in NO generation in MS medium or after U exposure.
Asunto(s)
Arabidopsis/metabolismo , Óxido Nítrico/farmacología , Arabidopsis/efectos de los fármacos , Arabidopsis/genética , Proteínas de Arabidopsis/efectos de los fármacos , Proteínas de Arabidopsis/genética , Proteínas de Arabidopsis/metabolismo , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Regulación de la Expresión Génica de las Plantas/genética , Mutación/genética , Uranio/farmacologíaRESUMEN
Ectomycorrhizal (EM) fungi form symbioses with dominant tree families in boreal, temperate and tropical ecosystems and are important drivers of ecosystem function. EM fungal hyphae extend over a large area making them susceptible to enhanced radiation levels from naturally occurring or anthropogenically originating radioisotopes in the rhizosphere. In this study, the in-vitro effects of ionizing radiation on the growth and biomass of EM fungi Suillus luteus, S. bovinus and Rhizopogon luteolus were investigated. EM fungal cultures were exposed to gamma radiation from a 137Cs source for 137â¯h in darkness at 21⯰C at dose rates of 404, 108.5 and 54.9â¯mGyâ¯h-1 resulting in total absorbed doses of 55.21, 14.82 and 7.50â¯Gy respectively. Cultures grown in the dark at 21⯰C but not exposed to the 137Cs source served as the control. Our results show that EM fungi vary in their sensitivity to ionizing radiation. EM fungi used in this study produced melanin and reactive oxygen species scavenging enzymes such as catalase and superoxide dismutase as a response to ionizing radiation.
Asunto(s)
Melaninas/metabolismo , Micorrizas/efectos de la radiación , Radiación Ionizante , Basidiomycota , Radioisótopos de Cesio , Ecosistema , Hongos , Micorrizas/enzimología , Micorrizas/crecimiento & desarrollo , Especies Reactivas de Oxígeno/metabolismoRESUMEN
Flooded (paddy) rice (Oryza sativa) can take up ions from the irrigation water by foliar uptake via the exposed stem base. We hypothesised that the stem base uptake of radiocaesium (RCs) is a pathway for rice grown in RCs-contaminated environments. We developed a bi-compartmental device which discriminates the stem base from root RCs uptake from solutions, thereby using RCs isotopes (137 Cs and 134 Cs) with < 2% solution leak between the compartments. Radiocaesium uptake was linear over time (0-24 h). Radiocaesium uptake to the entire plant, expressed per dry weight of the exposed parts, was sixfold higher for the roots than for the exposed stem base. At equal RCs concentrations in both compartments, the exposed stem base and root uptake contributed almost equally to the total shoot RCs concentrations. Reducing potassium supply to the roots not only increased the root RCs uptake but also increased RCs uptake by the stem base. This study was the first to experimentally demonstrate active and internally regulated RCs uptake by the stem base of rice. Scenario calculations for the Fukushima-affected area predict that RCs in irrigation water could be an important source of RCs in rice as indirectly suggested from field data.
Asunto(s)
Riego Agrícola , Radioisótopos de Cesio/metabolismo , Oryza/metabolismo , Hojas de la Planta/metabolismo , Contaminación Radiactiva del Agua/análisis , Cinética , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismo , Brotes de la Planta/efectos de los fármacos , Brotes de la Planta/metabolismo , Tallos de la Planta/efectos de los fármacos , Tallos de la Planta/metabolismo , Potasio/farmacología , SolucionesRESUMEN
When terrestrial environments get contaminated with long-lived gamma emitting radionuclides, plants that grow in these contaminated areas are exposed to gamma radiation during consecutive generations. Therefore it is important to evaluate the gamma induced stress response in plants in and between generations. The objective of this research is to reveal differences at the level of the antioxidative stress response between generations with a different radiation history. An experiment was conducted in which 7-days old Arabidopsis thaliana plants were exposed for 14 days to four different gamma dose rates: 22 mGy/h, 38 mGy/h, 86 mGy/h and 457 mGy/h. Two different plant groups were used: plants that were not exposed to gamma radiation before (P0) and plants that received the aforementioned gamma treatment during their previous generation (S1). Growth, the concentration of the antioxidants ascorbate and glutathione, a number of antioxidative enzyme activities and their gene transcript levels were analysed. A dose-rate dependent induction was seen for catalase (CAT) and guaiacol peroxidase (GPX) in the roots and for syringaldazine peroxidase (SPX) in the shoots. Differences between the two generations were observed for CAT and GPX in the roots, where a significantly higher activity of these reactive oxygen species (ROS) detoxifying enzymes was observed in the S1 generation. For SPX in the shoots, a dose dependent upregulation was observed in the P0 generation. However, high SPX activities were present for all doses in the S1 generation. These differences in enzyme activity between generations for SPX and GPX and the involvement of these enzymes in cell wall biosynthesis, suggest an important role for cell wall strengthening in the response to gamma irradiation.
Asunto(s)
Arabidopsis/fisiología , Arabidopsis/efectos de la radiación , Rayos gamma , Estrés Oxidativo/fisiología , Catalasa/metabolismo , Glutatión/metabolismo , Oxidación-Reducción , Peroxidasas/metabolismo , Raíces de Plantas/metabolismoRESUMEN
Long-lived radionuclides such as (90)Sr and (137)Cs can be naturally or accidentally deposited in the upper soil layers where they emit ß/γ radiation. Previous studies have shown that arbuscular mycorrhizal fungi (AMF) can accumulate and transfer radionuclides from soil to plant, but there have been no studies on the direct impact of ionizing radiation on AMF. In this study, root organ cultures of the AMF Rhizophagus irregularis MUCL 41833 were exposed to 15.37, 30.35, and 113.03 Gy gamma radiation from a (137)Cs source. Exposed spores were subsequently inoculated to Plantago lanceolata seedlings in pots, and root colonization and P uptake evaluated. P. lanceolata seedlings inoculated with non-irradiated AMF spores or with spores irradiated with up to 30.35 Gy gamma radiation had similar levels of root colonization. Spores irradiated with 113.03 Gy gamma radiation failed to colonize P. lanceolata roots. P content of plants inoculated with non-irradiated spores or of plants inoculated with spores irradiated with up to 30.35 Gy gamma radiation was higher than in non-mycorrhizal plants or plants inoculated with spores irradiated with 113.03 Gy gamma radiation. These results demonstrate that spores of R. irregularis MUCL 41833 are tolerant to chronic ionizing radiation at high doses.
Asunto(s)
Rayos gamma , Glomeromycota/efectos de la radiación , Fósforo/metabolismo , Plantago/metabolismo , Partículas beta , Glomeromycota/crecimiento & desarrollo , Glomeromycota/metabolismo , Micorrizas/efectos de la radiación , Fósforo/análisis , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Raíces de Plantas/microbiología , Plantago/microbiología , Radiación Ionizante , Plantones/microbiología , Suelo , Esporas Fúngicas/metabolismo , Esporas Fúngicas/efectos de la radiación , SimbiosisRESUMEN
Food chain contamination with radiocaesium (RCs) in the aftermath of the Fukushima accident calls for an analysis of the specific factors that control the RCs transfer. Here, soil-to-plant transfer factors (TF) of RCs for grass were predicted from the potassium concentration in soil solution (mK) and the Radiocaesium Interception Potential (RIP) of the soil using existing mechanistic models. The mK and RIP were (a) either measured for 37 topsoils collected from the Fukushima accident affected area or (b) predicted from the soil clay content and the soil exchangeable potassium content using the models that had been calibrated for European soils. An average ammonium concentration was used throughout in the prediction. The measured RIP ranged 14-fold and measured mK varied 37-fold among the soils. The measured RIP was lower than the RIP predicted from the soil clay content likely due to the lower content of weathered micas in the clay fraction of Japanese soils. Also the measured mK was lower than that predicted. As a result, the predicted TFs relying on the measured RIP and mK were, on average, about 22-fold larger than the TFs predicted using the European calibrated models. The geometric mean of the measured TFs for grass in the affected area (N = 82) was in the middle of both. The TFs were poorly related to soil classification classes, likely because soil fertility (mK) was obscuring the effects of the soil classification related to the soil mineralogy (RIP). This study suggests that, on average, Japanese soils are more vulnerable than European soils at equal soil clay and exchangeable K content. The affected regions will be targeted for refined model validation.
Asunto(s)
Radioisótopos de Cesio/análisis , Radioisótopos de Cesio/metabolismo , Plantas/metabolismo , Potasio/análisis , Monitoreo de Radiación , Suelo/química , Monitoreo del Ambiente , Europa (Continente) , Japón , Modelos Teóricos , Contaminantes Radiactivos del Suelo/análisis , Contaminantes Radiactivos del Suelo/metabolismoRESUMEN
Anthropogenic activities have led to a widespread uranium (U) contamination in many countries. The toxic effects of U at the cellular level have mainly been investigated at a pH around 5.5, the optimal pH for hydroponically grown plants. However, since the speciation of U, and hence its toxicity, is strongly dependent on environmental factors such as the pH, it is important to investigate the effects of U at different environmentally relevant pH levels. Although U is poorly translocated from the roots to the shoots, resulting in a low U concentration in the leaves, it has been demonstrated that toxic effects in the leaves were already visible after 1 day exposure at pH 5.5, although only when exposed to relatively high U concentrations (100 µM). Therefore, the present study aimed to analyse the effects of different U concentrations (ranging from 0 to 100 µM) at pH 4.5 in leaves of Arabidopsis thaliana plants. Results indicate that U induces early senescence in A. thaliana leaves as was suggested by a decreased expression of CAT2 accompanied by an induction of CAT3 expression, a decreased CAT capacity and an increased lipid peroxidation. In addition, miRNA398b/c is involved in the regulation of the SOD response in the leaves. As such, an increased MIR398b/c expression was observed leading to a decreased transcript level of CSD1/2. Finally, the biosynthesis of ascorbate was induced after U exposure. This can point towards an important role for this metabolite in the scavenging of reactive oxygen species under U stress.
Asunto(s)
Arabidopsis/efectos de la radiación , Estrés Oxidativo/efectos de la radiación , Uranio/toxicidad , Antioxidantes/metabolismo , Arabidopsis/metabolismo , Relación Dosis-Respuesta en la Radiación , Regulación de la Expresión Génica de las Plantas/efectos de la radiación , Concentración de Iones de Hidrógeno , Hojas de la Planta/metabolismo , Hojas de la Planta/efectos de la radiaciónRESUMEN
Human activity has led to an increasing amount of radionuclides in the environment and subsequently to an increased risk of exposure of the biosphere to ionising radiation. Due to their high linear energy transfer, α-emitters form a threat to biota when absorbed or integrated in living tissue. Among these, (241)Am is of major concern due to high affinity for organic matter and high specific activity. This study examines the dose-dependent biological effects of α-radiation delivered by (241)Am at the morphological, physiological and molecular level in 14-day old seedlings of Arabidopsis thaliana after hydroponic exposure for 4 or 7 days. Our results show that (241)Am has high transfer to the roots but low translocation to the shoots. In the roots, we observed a transcriptional response of reactive oxygen species scavenging and DNA repair pathways. At the physiological and morphological level this resulted in a response which evolved from redox balance control and stable biomass at low dose rates to growth reduction, reduced transfer and redox balance decline at higher dose rates. This situation was also reflected in the shoots where, despite the absence of a transcriptional response, the control of photosynthesis performance and redox balance declined with increasing dose rate. The data further suggest that the effects in both organs were initiated in the roots, where the highest dose rates occurred, ultimately affecting photosynthesis performance and carbon assimilation. Though further detailed study of nutrient balance and (241)Am localisation is necessary, it is clear that radionuclide uptake and distribution is a major parameter in the global exposure effects on plant performance and health.
Asunto(s)
Partículas alfa/efectos adversos , Americio/toxicidad , Antioxidantes/efectos de la radiación , Arabidopsis/efectos de la radiación , Daño del ADN , Transcripción Genética/efectos de la radiación , Antioxidantes/metabolismo , Arabidopsis/crecimiento & desarrollo , Arabidopsis/metabolismo , Reparación del ADN/efectos de la radiación , Relación Dosis-Respuesta en la Radiación , Estrés Oxidativo/efectos de la radiación , Fotosíntesis/efectos de la radiación , Raíces de Plantas/metabolismo , Raíces de Plantas/efectos de la radiación , Brotes de la Planta/metabolismo , Brotes de la Planta/efectos de la radiaciónRESUMEN
To evaluate the environmental impact of uranium (U) contamination, it is important to investigate the effects of U at ecologically relevant conditions. Since U speciation, and hence its toxicity, strongly depends on environmental pH, the present study aimed to investigate dose-dependent effects of U at pH 7.5. Arabidopsis thaliana plants (Mouse-ear Cress) were exposed for three days to different U concentrations at pH 7.5. In the roots, the increased capacities of ascorbate peroxidase and glutathione reductase indicate an important role for the ascorbate-glutathione cycle during U-induced stress. However, a significant decrease in the ascorbate redox state was observed after exposure to 75 and 100 µM U, indicating that those roots are severely stressed. In accordance with the roots, the ascorbate-glutathione cycle plays an important role in the antioxidative defence systems in A. thaliana leaves exposed to U at pH 7.5 as the ascorbate and glutathione biosynthesis were upregulated. In addition, small inductions of enzymes of the antioxidative defence system were observed at lower U concentrations to counteract the U-induced stress. However, at higher U concentrations it seems that the antioxidative defence system of the leaves collapses as reductions in enzyme activities and gene expression levels were observed.
Asunto(s)
Antioxidantes/metabolismo , Arabidopsis/efectos de los fármacos , Ácido Ascórbico/metabolismo , Glutatión/metabolismo , Uranio/farmacología , Arabidopsis/metabolismo , Proteínas de Arabidopsis/metabolismo , Relación Dosis-Respuesta a Droga , Regulación de la Expresión Génica de las Plantas/efectos de los fármacos , Concentración de Iones de Hidrógeno , Estrés Oxidativo , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismoRESUMEN
The high variability of the soil-to-plant transfer factor of radiocaesium (RCs) compels a detailed analysis of the radiocaesium interception potential (RIP) of soil, which is one of the specific factors ruling the RCs transfer. The range of the RIP values for agricultural soils in the Fukushima accident affected area has not yet been fully surveyed. Here, the RIP and other major soil chemical properties were characterised for 51 representative topsoils collected in the vicinity of the Fukushima contaminated area. The RIP ranged a factor of 50 among the soils and RIP values were lower for Andosols compared to other soils, suggesting a role of soil mineralogy. Correlation analysis revealed that the RIP was most strongly and negatively correlated to soil organic matter content and oxalate extractable aluminium. The RIP correlated weakly but positively to soil clay content. The slope of the correlation between RIP and clay content showed that the RIP per unit clay was only 4.8 mmol g(-1) clay, about threefold lower than that for clays of European soils, suggesting more amorphous minerals and less micaceous minerals in the clay fraction of Japanese soils. The negative correlation between RIP and soil organic matter may indicate that organic matter can mask highly selective sorption sites to RCs. Multiple regression analysis with soil organic matter and cation exchange capacity explained the soil RIP (R(2)=0.64), allowing us to map soil RIP based on existing soil map information.
Asunto(s)
Radioisótopos de Cesio/análisis , Contaminantes Radiactivos del Suelo/análisis , Suelo/química , Adsorción , Silicatos de Aluminio/química , Radioisótopos de Cesio/química , Arcilla , Japón , Minerales/química , Contaminantes Radiactivos del Suelo/químicaRESUMEN
Two sequential extraction procedures were carried out on six soils with different chemical properties and contamination history to estimate the partitioning of uranium (U) between different soil fractions. The first standard method (method of Schultz) was specifically developed for actinides, while the second one (method of Rauret) was initially created for heavy metals. Reproducibility of both methods was compared by means of the coefficient of variation (CV). A soil-to-plant transfer experiment was also carried out with ryegrass to verify if one of the extracted fractions efficiently predicted plant uptake. In artificially contaminated soils, most of the U was retrieved from the exchangeable and the carbonates fractions. In soils with high natural levels of U or contaminated by industrial activity, most of the U was found in the less available fractions. Different U concentrations were found in the fractions which were supposed to be comparable in the two methods. Extracted fractions following Schultz differentiated more strongly between the tested soils but no relationships with soil parameters could be established. As expected, the highest U transfer factors (TF) were observed for ryegrass grown on artificially contaminated soils and the lowest on soils with high natural concentrations or industrial contamination, in agreement with the extraction procedures. No good relation was found between the soil-to-shoot TF and the extracted U concentrations. On the other hand, the U concentration in the roots, the U concentration in the shoots and the soil-to-root TF are well correlated to the U concentration determined in the first extracted fractions (so called exchangeable fractions) from the method of Schultz. We conclude that the extraction method according to Schultz should be preferably used for U, and that the exchangeable fraction can be proposed as a potential indicator to evaluate plant uptake in soils.
Asunto(s)
Fraccionamiento Químico/métodos , Lolium/metabolismo , Contaminantes Radiactivos del Suelo/análisis , Contaminantes Radiactivos del Suelo/metabolismo , Uranio/análisis , Uranio/metabolismo , Reproducibilidad de los Resultados , Suelo/químicaRESUMEN
To study the impact of environmental uranium (U) contamination, effects should be analysed at different environmentally relevant pH levels as the speciation of U, and hence its toxicity, is strongly dependent on the pH. As photosynthesis is a major energy producing process in plants intimately connected to plant growth and known to be susceptible to metal stress, the effects of different U concentrations on photosynthesis in 18-day-old Arabidopsis thaliana (Columbia ecotype) are investigated at two contrasting pH levels, pH 4.5 and pH 7.5. At pH 4.5, U is highly taken up by the roots but is poorly translocated to the shoots, while at pH 7.5, less U is taken up but the translocation is higher. The lower U concentrations in the shoots at pH 4.5 are accompanied by a more reduced leaf growth as compared to pH 7.5. In addition, U does not influence the photosynthetic machinery at pH 7.5, while an optimization of the photosynthesis takes place after U exposure at pH 4.5. As such, more of the absorbed quanta are effectively used for photosynthesis accompanied by a decreased non-photochemical quenching and an increased electron transport rate. Since the enhanced photosynthesis at pH 4.5 is accompanied by a decreased growth, we suggest that the energy produced during photosynthesis is used for defence reactions against U-induced oxidative stress rather than for growth. As such, a high discrepancy was observed between the two pH levels, with an optimized photosynthetic apparatus at pH 4.5 and almost no effects at pH 7.5.
Asunto(s)
Arabidopsis/efectos de los fármacos , Arabidopsis/metabolismo , Fotosíntesis/efectos de los fármacos , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismo , Uranio/farmacología , Concentración de Iones de HidrógenoRESUMEN
As the environment is inevitably exposed to ionizing radiation from natural and anthropogenic sources, it is important to evaluate gamma radiation induced stress responses in plants. The objective of this research is therefore to investigate radiation effects in Arabidopsis thaliana on individual and subcellular level by exposing 2-weeks-old seedlings for 7 days to total doses of 3.9 Gy, 6.7 Gy, 14.8 Gy and 58.8 Gy and evaluating growth, photosynthesis, chlorophyll a, chlorophyll b and carotenoid concentrations and antioxidative enzyme capacities. While the capacity of photosystem II (PSII measured as Fv/Fm) remained intact, plants started optimizing their photosynthetic process at the lower radiation doses by increasing the PSII efficiency (φPSII) and the maximal electron transport rate (ETRmax) and by decreasing the non-photochemical quenching (NPQ). At the highest radiation dose, photosynthetic parameters resembled those of control conditions. On subcellular level, roots showed increased superoxide dismutase (SOD) and ascorbate peroxidase (APX) capacities under gamma irradiation but catalase (CAT), syringaldazine peroxidase (SPX) and guaiacol peroxidase (GPX) activities, on the other hand, decreased. In the leaves no alterations were observed in SOD, CAT and SPX capacities, but GPX was highly affected. Based on these results it seems that roots are more sensitive for oxidative stress under gamma radiation exposure than leaves.
Asunto(s)
Arabidopsis/efectos de la radiación , Rayos gamma/efectos adversos , Arabidopsis/crecimiento & desarrollo , Arabidopsis/metabolismo , Carotenoides/metabolismo , Catalasa/metabolismo , Clorofila/metabolismo , Clorofila A , Peroxidasas/metabolismo , Fotosíntesis/efectos de la radiación , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/metabolismo , Hojas de la Planta/efectos de la radiación , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Raíces de Plantas/efectos de la radiación , Estrés Fisiológico/fisiología , Superóxido Dismutasa/metabolismoRESUMEN
There is a need for a better understanding of biological effects of radiation exposure in non-human biota. Correct description of these effects requires a more detailed model of dosimetry than that available in current risk assessment tools, particularly for plants. In this paper, we propose a simple model for dose calculations in roots and shoots of Arabidopsis thaliana seedlings exposed to radionuclides in a hydroponic exposure setup. This model is used to compare absorbed doses for three radionuclides, (241)Am (α-radiation), (90)Sr (ß-radiation) and (133)Ba (γ radiation). Using established dosimetric calculation methods, dose conversion coefficient values were determined for each organ separately based on uptake data from the different plant organs. These calculations were then compared to the DCC values obtained with the ERICA tool under equivalent geometry assumptions. When comparing with our new method, the ERICA tool appears to overestimate internal doses and underestimate external doses in the roots for all three radionuclides, though each to a different extent. These observations might help to refine dose-response relationships. The DCC values for (90)Sr in roots are shown to deviate the most. A dose-effect curve for (90)Sr ß-radiation has been established on biomass and photosynthesis endpoints, but no significant dose-dependent effects are observed. This indicates the need for use of endpoints at the molecular and physiological scale.
Asunto(s)
Arabidopsis/efectos de la radiación , Radiación Ionizante , Radioisótopos/efectos adversos , Partículas alfa/efectos adversos , Partículas beta/efectos adversos , Relación Dosis-Respuesta en la Radiación , Rayos gamma/efectos adversosRESUMEN
Uranium (U) causes oxidative stress in Arabidopsis thaliana plants grown at pH 5.5. However, U speciation and its toxicity strongly depend on environmental parameters, for example pH. It is unknown how different U species determine U uptake and translocation within plants and how they might affect the oxidative defense mechanisms of these plants. The present study analyzed U uptake and oxidative stress-related responses in A. thaliana (Columbia ecotype) under contrasted U chemical speciation conditions. The 18-d-old seedlings were exposed for 3 d to 25 µM U in a nutrient solution of which the pH was adjusted to 4.5, 5.5, 6.5, or 7.5. Results indicate that there is a different rate of U uptake and translocation at the different pHs, with high uptake and low translocation at low pH and lower uptake but higher translocation at high pH. After U exposure, an increased glutathione reductase activity and total glutathione concentration were observed in U-exposed roots, pointing toward an important role for glutathione in the root defense system against U either by chelation or by antioxidative defense mechanisms. In leaves, antioxidative defense mechanisms were activated on U exposure, indicated by increased superoxide dismutase and catalase activity. As it seems that U toxicity is influenced by pH, it is important to consider site-specific characteristics when making U risk assessments.
Asunto(s)
Arabidopsis/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Uranio/metabolismo , Antioxidantes/metabolismo , Arabidopsis/metabolismo , Glutatión/metabolismo , Concentración de Iones de Hidrógeno , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/metabolismo , Plantones/efectos de los fármacos , Plantones/metabolismo , Superóxido Dismutasa/metabolismo , Uranio/farmacologíaRESUMEN
When aiming to evaluate the environmental impact of uranium contamination, it is important to unravel the mechanisms by which plants respond to uranium stress. As oxidative stress seems an important modulator under other heavy metal stress, this study aimed to investigate oxidative stress related responses in Arabidopsis thaliana exposed to uranium concentrations ranging from 0.1 to 100 µM for 1, 3 and 7 days. Besides analyzing relevant reactive oxygen species-producing and -scavenging enzymes at protein and transcriptional level, the importance of the ascorbate-glutathione cycle under uranium stress was investigated. These results are reported separately for roots and leaves in two papers: Part I dealing with responses in the roots and Part II unraveling responses in the leaves and presenting general conclusions. Results of Part I indicate that oxidative stress related responses in the roots were only triggered following exposure to the highest uranium concentration of 100 µM. A fast oxidative burst was suggested based on the observed enhancement of lipoxygenase (LOX1) and respiratory burst oxydase homolog (RBOHD) transcript levels already after 1 day. The first line of defense was attributed to superoxide dismutase (SOD), also triggered from the first day. The enhanced SOD-capacity observed at protein level corresponded with an enhanced expression of iron SOD (FSD1) located in the plastids. For the detoxification of H(2)O(2), an early increase in catalase (CAT1) transcript levels was observed while peroxidase capacities were enhanced at the later stage of 3 days. Although the ascorbate peroxidase capacity and gene expression (APX1) increased, the ascorbate/dehydroascorbate redox balance was completely disrupted and shifted toward the oxidized form. This disrupted balance could not be inverted by the glutathione part of the cycle although the glutathione redox balance could be maintained.
Asunto(s)
Antioxidantes/metabolismo , Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Estrés Oxidativo , Uranio/toxicidad , Arabidopsis/efectos de los fármacos , Arabidopsis/crecimiento & desarrollo , Ácido Ascórbico/metabolismo , Expresión Génica , Glutatión/metabolismo , Peróxido de Hidrógeno/metabolismo , Oxidación-Reducción , Oxidorreductasas/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Dosis de Radiación , Especies Reactivas de Oxígeno/metabolismo , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Plantones/metabolismoRESUMEN
The cellular redox balance seems an important modulator under heavy metal stress. While for other heavy metals these processes are well studied, oxidative stress related responses are also known to be triggered under uranium stress but information remains limited. This study aimed to further unravel the mechanisms by which plants respond to uranium stress. Seventeen-day-old Arabidopsis thaliana seedlings, grown on a modified Hoagland solution under controlled conditions, were exposed to 0, 0.1, 1, 10 and 100 µM uranium for 1, 3 and 7 days. While in Part I of this study oxidative stress related responses in the roots were discussed, this second Part II discusses oxidative stress related responses in the leaves and general conclusions drawn from the results of the roots and the leaves will be presented. As several responses were already visible following 1 day exposure, when uranium concentrations in the leaves were negligible, a root-to-shoot signaling system was suggested in which plastids could be important sensing sites. While lipid peroxidation, based on the amount of thiobarbituric acid reactive compounds, was observed after exposure to 100 µM uranium, affecting membrane structure and function, a transient concentration dependent response pattern was visible for lipoxygenase initiated lipid peroxidation. This transient character of uranium stress responses in leaves was emphasized by results of lipoxygenase (LOX2) and antioxidative enzyme transcript levels, enzyme capacities and glutathione concentrations both in time as with concentration. The ascorbate redox balance seemed an important modulator of uranium stress responses in the leaves as in addition to the previous transient responses, the total ascorbate concentration and ascorbate/dehydroascorbate redox balance increased in a concentration and time dependent manner. This could represent either a slow transient response or a stable increase with regard to plant acclimation to uranium stress.
Asunto(s)
Proteínas de Arabidopsis/metabolismo , Arabidopsis/metabolismo , Estrés Oxidativo , Uranio/toxicidad , Antioxidantes/metabolismo , Arabidopsis/efectos de los fármacos , Arabidopsis/crecimiento & desarrollo , Ácido Ascórbico/metabolismo , Expresión Génica , Glutatión/metabolismo , Peróxido de Hidrógeno/metabolismo , Peroxidación de Lípido , Oxidación-Reducción , Oxidorreductasas/metabolismo , Hojas de la Planta/efectos de los fármacos , Hojas de la Planta/crecimiento & desarrollo , Hojas de la Planta/metabolismo , Raíces de Plantas/efectos de los fármacos , Raíces de Plantas/crecimiento & desarrollo , Raíces de Plantas/metabolismo , Dosis de Radiación , Especies Reactivas de Oxígeno/metabolismo , Plantones/efectos de los fármacos , Plantones/crecimiento & desarrollo , Plantones/metabolismoRESUMEN
In this study, toxicity effects in plants of uranium in a binary pollution condition were investigated by studying biological responses and unraveling oxidative stress related mechanisms in Arabidopsis thaliana seedlings, grown on hydroponics and exposed for 3 days to 10 µM uranium in combination with 5 µM cadmium. While uranium mostly accumulated in the roots with very low root-to-shoot transport, cadmium was taken up less by the roots but showed higher translocation to the shoots. Under mixed exposure, cadmium influenced uranium uptake highly but not the other way round resulting in a doubled uranium concentration in the roots. Under our mixed exposure conditions, it is clear that micronutrient concentrations in the roots are strongly influenced by addition of cadmium as a second stressor, while leaf macronutrient concentrations are mostly influenced by uranium. Oxidative stress related responses are highly affected by cadmium while uranium influence is more limited. Hereby, an important role was attributed to the ascorbate redox balance together with glutathione as both metabolites, but more explicitly for ascorbate, increased their reduced form, indicating an important defense and regulatory function. While for roots, based on an increase in FSD1 gene expression, oxidative stress was suggested to be superoxide induced, in leaves on the other hand, hydrogen peroxide related genes were mostly altered.
Asunto(s)
Adaptación Fisiológica , Antioxidantes/metabolismo , Arabidopsis/metabolismo , Cadmio/toxicidad , Genes de Plantas , Estrés Oxidativo/efectos de los fármacos , Uranio/toxicidad , Adaptación Fisiológica/genética , Arabidopsis/genética , Ácido Ascórbico/metabolismo , Cadmio/metabolismo , Expresión Génica , Glutatión/metabolismo , Peróxido de Hidrógeno , Hidroponía , Micronutrientes/metabolismo , Oxidación-Reducción , Estrés Oxidativo/genética , Estructuras de las Plantas/metabolismo , Plantones/metabolismo , Superóxido Dismutasa/genética , Superóxido Dismutasa/metabolismo , Superóxidos/metabolismo , Uranio/metabolismoRESUMEN
Uranium never occurs as a single pollutant in the environment, but always in combination with other stressors such as ionizing radiation. As effects induced by multiple contaminants can differ markedly from the effects induced by the individual stressors, this multiple pollution context should not be neglected. In this study, effects on growth, nutrient uptake and oxidative stress induced by the single stressors uranium and gamma radiation are compared with the effects induced by the combination of both stressors. By doing this, we aim to better understand the effects induced by the combined stressors but also to get more insight in stressor-specific response mechanisms. Eighteen-day-old Arabidopsis thaliana seedlings were exposed for 3 days to 10 muM uranium and 3.5 Gy gamma radiation. Gamma radiation interfered with uranium uptake, resulting in decreased uranium concentrations in the roots, but with higher transport to the leaves. This resulted in a better root growth but increased leaf lipid peroxidation. For the other endpoints studied, effects under combined exposure were mostly determined by uranium presence and only limited influenced by gamma presence. Furthermore, an important role is suggested for CAT1/2/3 gene expression under uranium and mixed stressor conditions in the leaves.