RESUMEN

Ultrafiltration-based polyacrylonitrile membranes (UF-membranes) were evaluated for their ability to retain tissue culture adapted indigenous hepatitis A virus from water. Amicon cell (dead-end ultrafiltration unit)-based experiment was set up and viral assay was carried out using rapid and sensitive reverse transcriptase polymerase chain reaction (RT-PCR). The RT-PCR results show retention of virus particles by the polyacrylonitrile membranes. A protocol for routine virological evaluation of membranes is described and the use of these membranes for water purification units and for virus concentration systems for field application is discussed.

Asunto(s)

Virus de la Hepatitis A/aislamiento & purificación , Microbiología del Agua , Resinas Acrílicas , Secuencia de Bases , ADN Viral/genética , Virus de la Hepatitis A/genética , Membranas Artificiales , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Ultrafiltración/métodos , Virología/métodos , Abastecimiento de Agua