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Objective To evaluate the surgical technique and experience of endoscopic endonasal transsphenoidal approach combined with drill in surgical treatment of pituitary adenomas.Methods We retrospectively analyzed the clinical data of 29 patients suffered from pituitary adenomas,collected from September 2007 to August 2011 in our hospital,and the surgical technique and experience of endoscopic endonasal transsphenoidal approach in treating them. Results Total resection was achieved in 19 patients (65.5％),subtotal resection in 8 (27.6％) and partial resection in 2 (6.9％).Cerebrospinal leak appeared in 4 patients and temporary diatetes insipidus in 27 patients, and all these complications were controlled after treatment. Follow-up was performed for 3-8 months; the acuity of vision was improved in 15 patients (83.33％); the defect of visual field was improved in 8 (80％); headache was disappeared or relieved in 9 (81.82％).The high preoperative prolactin (PRL) level in 15 patients was obviously decreased from ([304.55+181.30] μg/L) to ([43.27+28.75] μg/L) 3 months after the surgery (P＜0.05); the high preoperative growth hormone (GH) level in 6 patients was obviously decreased from ([48.16+22.36] ng/L) to ([14.03+4.57] ng/L) 3 months afterthesurgery (P＜0.05).Conclusion Neuroendoscopic surgery combined with drill via endonasal transsphenoidal approach in the treatment of pituitary adenomas is a safe,minimally invasive and efficient procedure.
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Objective To construct an adenoviral vector containing mouse Hes1 gene, observe its expression in the hippocampus of adult mice and build a basis for further investigation of Hes1 gene in adult neurogenesis. Methods The restriction endonuclease was used to digest plasmid pEGFP-mHes1 and pDC316, and then, the products were recovered and connected by T4 DNA ligase and the shuttle plasmid pDC316-mHes1 was constructed which was identified by the method of PCR and EcoRI+HindⅢ digestion. After that, the shuttle plasmid pDC316-mHes1 was cotransfected into 293 cells with the adenovirus skeleton plasmid pBHGlox_E1,3Cre to obtain the produced replication defective recombinant adenovirus Ad5-mHes1. Then, the recombinant adenovirus could be further amplified and purified. The report recombinant adenoviruses were Ad5-EGFP containing enhanced green fluorescent protein (EGFP).Then, Ad5-mHes1 and Ad5-EGFP were stereotactic injected into the hippocampus of the adult C57BL/6 mice and their expressions in the hippocampus were detected. Western blotting was used to detect the Hes1 protein level 7 d after the injection. Fluorescent microscopy was used to observe the expression of EGFP in the hippocampus. Results The experimental results of identification by the methods of PCR and EcoRI+HindⅢ digestion were in accordance with the anticipated results, and the sequences were also the same with mHeslCDS sequences; Hes1 gene was expressed in the hippocampus of both the PBS injection group and the Ad5-mHes1 injection group 7 d after the injection, and the expression of Hes1/GAPDH in Ad5-mHes1 injection hippocampus (0.705 ±0.128) was statistically different as compared with that in PBS injection group (0.363±0.053, P＜0.05). Ad5-EGFP strongly expressed in the granular cell layer and subgranular zone (SGZ) of dentate gyrus. Conclusion The adenoviral vector of mouse Hes1 gene is successfully established and Hes1 gene is expressed in the hippocampus of C57BL/6 adult mice.