RESUMEN

Hydrazine is volatile and highly toxic, causing severe harm to water, soil, air, and organisms. Therefore, real-time detection and long-term monitoring of hydrazine are crucial for environmental protection and human health. Herein, an "OFF-ON" fluorescent probe 5-((10-ethyl-2-methoxy-10 H-phenothiazin-3-yl)methylene)-2,2-dimethyl-1,3-dioxane-4,6-dione (MPD) for hydrazine detection through a nucleophilic addition reaction was developed. MPD could exclusively identify hydrazine through colorimetric and fluorescent dual-channel responses within 30 s, which also demonstrated high sensitivity (detection limit, 12 nM) and a wide pH range (6 -12). The sensing mechanism of MPD was confirmed using theoretical calculations, where fluorescence was emitted following the recognition of hydrazine because of the disappearance of the photoinduced electron transfer (PET) process. Using a smartphone, MPD enabled the quantitative detection of hydrazine in real water samples and sandy soil. Notably, in the process of detecting hydrazine in actual water samples, the establishment of analytical methods and the completion of rapid quantitative detection only required a smartphone and built-in apps. Additionally, we showed that MPD could recognize hydrazine in various environmental samples, including plants, food, hydrazine vapors, and cells. We believe that the fluorescent probe MPD developed in this study and the established smartphone visualization platform will provide a convenient and effective tool for detecting hydrazine in environmental monitoring, food safety assessment, biological system safety, and other fields.

RESUMEN

The signal recognition particle (SRP) system delivers approximately 30% of the proteome to the endoplasmic reticulum (ER) membrane. SRP receptor alpha (SRα) binds to SRP for targeting nascent secreted proteins to the ER membrane in eukaryotic cells. In this study, the SRα homologous gene was identified in the sea cucumber, Apostichopus japonicus (AjSRα). AjSRα codes for 641 amino acids and has 54.94% identity with its mammalian homologs. Like mammalian SRα, it is expected to contain the SRP-alpha N domain, SRP54_N domain, and SRP54 domain. In addition, AjSRα is ubiquitously expressed in adult tissues and exhibits a sexually dimorphic expression pattern, with significantly higher expression in ovaries compared to testes. As a maternal factor, AjSRα can be continuously detected during embryonic development. Importantly, we first attempted to investigate its function by using lentiviral vectors for delivering SRα gene-specific shRNA, and we revealed that lentiviral vectors do not induce an upregulation of immune-related enzymes in sea cucumbers. However, compared to the dsRNA-based RNA interference (RNAi) method, lentivirus-mediated RNAi caused dynamic changes in gene expression at a later time. This study supplied the technical support for studying the functional mechanism of SRα in sea cucumbers.

Asunto(s)

Pepinos de Mar , Animales , Pepinos de Mar/genética , Pepinos de Mar/metabolismo , Receptores Citoplasmáticos y Nucleares/metabolismo , Retículo Endoplásmico/metabolismo , Receptores de Péptidos/metabolismo , Partícula de Reconocimiento de Señal/metabolismo , Mamíferos/metabolismo

RESUMEN

BACKGROUND: The purpose of this study was to explore the clinical effect of the anterior approach to the lower cervical spine for cervicothoracic spinal tuberculosis (CTSTB). METHODS: A total of 8 patients (6 males and 2 females) with CTSTB diagnosed by imaging examination and γ-interferon test, underwent the first stage lesion removal, bone grafting and internal fixation through the anterior approach to the lower cervical spine, were studied. Then, the clinical efficacy was evaluated using statistical analysis based on the materials about the Cobb angle of kyphosis, visual analog scale (VAS), Frankel grade, erythrocyte sedimentation rate (ESR), and C-reactive protein (CRP). RESULTS: All patients' age ranged from 28 to 64 years (mean 46.2 ±â€Š16.3). The mean operation time was 145 minutes (range 90-180 mins), intraoperative blood loss was 425 mL (range 200-1000 mL), and the average bone fusion time was 7.4 months. Postoperative neurological function of Frankel grading was significantly improved compared with that of preoperative. At final follow-up, the kyphosis angle was significantly decreased to 10 ±â€Š2.1°, the mean VAS score was 1.6 ±â€Š0.9 showing significant improvement, ESR and CRP returned to normal. The incidence of complications within 3 months after surgery was 25%, and the incidence of complications directly related to surgery was 12.5% (cerebrospinal fluid leakage). CONCLUSION: On the basis of familiarity with the anatomical structure and combining with the experience of the surgeon, the anterior approach to the lower cervical spine can be an effective treatment method for CTSTB.

Asunto(s)

Vértebras Torácicas/diagnóstico por imagen , Tuberculosis de la Columna Vertebral/diagnóstico por imagen , Adulto , Trasplante Óseo , Femenino , Fijación Interna de Fracturas , Humanos , Cifosis , Masculino , Persona de Mediana Edad , Tempo Operativo , Periodo Posoperatorio , Procedimientos de Cirugía Plástica , Vértebras Torácicas/cirugía , Resultado del Tratamiento , Tuberculosis de la Columna Vertebral/cirugía , Escala Visual Analógica

RESUMEN

We report a combined experimental and computational study of the large self-assembly complex (CoL)(2) [L= bis(2,4,8,10-tetramethyl-9-methoxycarbonylethyldipyrrin-3-yl)methane] containing 172 atoms. An extensive density functional theory (DFT) and time-dependent DFT study of this complex in gas phase and in CH(2)Cl(2) solution was performed, investigating the effect of substitutions of methyl and methyl propionate on the electronic structure and optical properties of this complex. The calculated IR and Raman spectra are in excellent agreement with the experiment, thus allowing a detailed assignment of the vibrational absorption bands. Comparing the vibrational spectrum of (CoL)(2) with that of (ZnL')(2) [L' = bis(2,4-dimethyldipyrrin-3-yl)methane], the substitution of methyl on the C(beta) atom results in sizable shifts on the same modes; particularly in the case of mode upsilon(C(beta)-C(beta)), the shift is more than 20 cm(-1). The lowest 70 singlet-singlet spin-allowed excited states were taken into account for the calculation by TDDFT in gas phase and PCM-TDDFT in CH(2)Cl(2) solution. Theoretical calculations provide a good description on positions of the two band maximums in observed spectrum but predict a contrary relatively intensity for these two bands. In the UV-vis absorption spectrum of (CoL)(2) complex, the band maximum at 525.5 nm is mainly attributed to the pi-->pi* transition. The band maximum at 488.1 nm is originated from metal-ligand charge-transfer (MLCT) transition mixed with interligand pi-->pi* transition.