RESUMEN
OBJECTIVE: To investigate the damaging of human umbilical vein endothelial cells (HUVEC) induced by antiplatelet integrin ß3 antibodies in vitro. METHODS: The serum from 36 chronic ITP patients were collected, flow cytometry and monoclonal antibody specific immobilization of platelet antigen (MAIPA) assay were used to collect antiplatelet integrin ß3 antibodies from the serum of the patients. After HUVEC were treated by ITP patient serum (PS) containing anti-integrin ß3 antibodies, the cell damage was detected by Lactate dehydrogenase (LDH) assay, cell apoptosis was detected by flow cytometry, the expression of apoptosis-related gene Bax was detected by Reverse transcription-Quantitative real-time PCR (RT-qPCR), and expression of Apoptosis-related signaling pathway protein Akt and related protein Bax were detected by Western blot. HUVEC were treated by PS combined with Akt activator SC79, the cells damage were detected by LDH assay, apoptosis of the cells were detected by flow cytometry, the expression of apoptosis-related gene Bax was detected by RT-qPCR. RESULTS: Among 36 cases of serum from the chronic ITP patients, 5 patients' serum containing anti-integrin ß3 antibodies were collected. After HUVEC was treated by PS, the viability of LDH was significant increasedï¼P<0.05ï¼, so as for the apoptosis of the cellsï¼P<0.05ï¼, the expression of gene and protein of Bax was increased up-regulatedï¼P<0.05ï¼, the protein expression of pAkt was down-regulatedï¼P<0.05ï¼. Comparing with HUVEC cultured with PS alone, the viability of LDH of HUVEC treated by PS combined with SC79 was significantly reducedï¼P<0.05ï¼, so as for the apoptosis of the cellsï¼P<0.05ï¼, and gene expression of Bax was significantly decreasedï¼P<0.05ï¼. CONCLUSION: Anti-integrin ß3 serum can cause the damage and apoptosis of HUVEC through Akt signaling pathwayï¼the apoptotic effects of anti-integrin ß3 antibodies to HUVEC was effectively reversed by SC79.
Asunto(s)
Apoptosis , Integrina beta3 , Células Cultivadas , Células Endoteliales de la Vena Umbilical Humana , Humanos , Transducción de SeñalRESUMEN
Side population (SP) cells are a small subpopulation of cells found in many mammalian tissues and organs, identified by their capacity to efflux Hoechst 33342 dye. They are enriched for stem/progenitor cell activity. SP cells isolated from the adult mouse lung can be separated into a CD45+ subset (bone marrowderived) and a CD45 subset that can be subdivided into CD31 and CD31+ subpopulations. CD45/CD31 lung SP (LSP) cells are known to be mesenchymal stem cells. However, CD45/CD31+ LSP cells are not fully characterized. In the present study, it was found that CD45/CD31+ LSP cells were able to form colonies. Based on the expression of vascular endothelial growth factor receptor 2 (VEGFR2), these cells were separated into VEGFR2 and VEGFR2+ cells. The CD45/CD31+/VEGFR2 LSP cells expressed genes characteristic of smooth muscle and endothelial progenitors, and were able to differentiate into smooth muscle and endothelial cells in vitro. The CD45/CD31+/VEGFR2+ LSP cells expressed genes characteristic of endothelial progenitors and gave rise to endothelial cells, although not smooth muscle, in vitro. The data demonstrate that CD45/CD31+/VEGFR2 LSP cells differentiated into CD45/CD31+/VEGFR2+ LSP cells and then endothelial cells, indicating that CD45/CD31+/VEGFR2+ LSP cells are likely to be derived from CD45/CD31+/VEGFR2 LSP cells. Taken together, the results suggest that CD45/CD31+ LSP cells can be separated into CD45/CD31+/VEGFR2 LSP cells, which may be progenitors of endothelial and smooth muscle, whereas CD45/CD31+/VEGFR2+ LSP cells may serve as late commitment endothelial progenitors in the adult mouse lung.
Asunto(s)
Diferenciación Celular , Células Endoteliales/citología , Células Endoteliales/metabolismo , Pulmón/citología , Miocitos del Músculo Liso/citología , Miocitos del Músculo Liso/metabolismo , Células de Población Lateral/citología , Células de Población Lateral/metabolismo , Animales , Biomarcadores , Diferenciación Celular/genética , Línea Celular Tumoral , Separación Celular/métodos , Células Cultivadas , Ensayo de Unidades Formadoras de Colonias , Femenino , Técnica del Anticuerpo Fluorescente , Expresión Génica , Inmunofenotipificación , Antígenos Comunes de Leucocito/genética , Antígenos Comunes de Leucocito/metabolismo , Células Madre Mesenquimatosas/citología , Células Madre Mesenquimatosas/metabolismo , Ratones , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/genética , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/metabolismoRESUMEN
BACKGROUND: Side-population (SP) cells, identified by their capacity to efflux Hoechst dye, are highly enriched for stem/progenitor cell activity. They are found in many mammalian tissues, including mouse heart. Studies suggest that cardiac SP (CSP) cells can be divided into SCA1+/CD31-, SCA1+/CD31+ and SCA1-/CD31- CSP subpopulations. SCA1+/CD31- were shown to be cardiac and endothelial stem/progenitors while SCA1+/CD31+ CSP cells are endothelial progenitors. SCA1-/CD31- CSP cells remain to be fully characterized. In this study, we characterized SCA1-/CD31- CSP cells in the adult mouse heart, and investigated their abilities to proliferate, differentiate and migrate in vitro and in vivo. METHODS AND RESULTS: Using fluorescence-activated cell sorting, reverse transcriptase/polymerase chain reaction, assays of cell proliferation, differentiation and migration, and a murine model of myocardial infarction we show that SCA1-/CD31- CSP cells are located in the heart mesenchyme and express genes characteristic of stem cells and endothelial progenitors. These cells were capable of proliferation, differentiation, migration and vascularization in vitro and in vivo. Following experimental myocardial infarction, the SCA1-/CD31- CSP cells migrated from non-infarcted areas to the infarcted region within the myocardium where they differentiated into endothelial cells forming vascular (tube-like) structures. We further demonstrated that the SDF-1α/CXCR4 pathway may play an important role in migration of these cells after myocardial infarction. CONCLUSIONS: Based on their gene expression profile, localization and ability to proliferate, differentiate, migrate and vascularize in vitro and in vivo, we conclude that SCA1-/CD31- CSP cells may serve as endothelial progenitor cells in the adult mouse heart.
Asunto(s)
Ataxina-1/fisiología , Células Endoteliales/fisiología , Infarto del Miocardio/patología , Miocitos Cardíacos/fisiología , Molécula-1 de Adhesión Celular Endotelial de Plaqueta/fisiología , Células de Población Lateral/fisiología , Animales , Técnicas de Cultivo de Célula , Diferenciación Celular , Movimiento Celular , Proliferación Celular , Modelos Animales de Enfermedad , Femenino , Ratones , Ratones Endogámicos C57BL , Infarto del Miocardio/etiologíaRESUMEN
The aim of this study was to search for a good inducer agent using for cardiomyogenic differentiation of stem cells. Human placenta-derived mesenchymal stem cells (hPDMSCs) were isolated and incubated in enriched medium. Fourth passaged cells were treated with 10mg/L dan-shen root for 20 days. Morphologic characteristics were analyzed by confocal and electron microscopy. Expression of α-sarcomeric actin was analyzed by immunohistochemistry. Expression of cardiac troponin-I (TnI) was analyzed by immunohistofluorescence. Atrial natriuretic factor (ANF) and beta-myocin heavy chain (ß-MHC) were detected by reverse transcriptase polymerase chain reaction (RT-PCR). hPDMSCs treated with dan-shen root gradually formed a stick-like morphology and connected with adjoining cells. On the 20th day, most of the induced cells stained positive with α-sarcomeric actin and TnI antibody. ANF and ß-MHC were also detected in the induced cells. Approximately 80% of the cells were successfully transdifferentiated into cardiomyocytes. In conclusion, dan-shen root is a good inducer agent used for cardiomyogenic differentiation of hPDMSCs.