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PURPOSE: EGFR classical mutations respond well to EGFR tyrosine kinase inhibitors. However, it is uncertain whether currently available EGFR-TKIs are effective against rare EGFR mutations and compound mutations. Herein, the effectiveness of almonertinib and alflutinib, the third-generation tyrosine kinase inhibitors developed in China, on rare EGFR S768I mutations and compound mutations is identified. METHODS: In this study, using CRISPR method, four EGFR S768I mutation cell lines were constructed, and the sensitivity of EGFR to almonertinib and alflutinib was tested, with positive controls being the 1st (gefitinib), 2nd (afatinib), and 3rd (osimertinib) generation drugs. RESULTS: The present results indicate that almonertinib and alflutinib can effectively inhibit cell viability and proliferation in rare EGFR S768I mutations through the ERK or AKT pathways in a time-dependent manner, by blocking the cell cycle and inhibiting apoptosis. CONCLUSIONS: These findings suggest that almonertinib and alflutinib may be potential therapeutic options for non-small cell lung cancer patients with the EGFR S768I mutation.

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Gastric cancer is one of the most prevalent malignant tumors worldwide, characterized by high incidence and mortality rates. At present, comprehensive surgical treatment has enhanced the prognosis of locally advanced gastric cancer patients significantly. However, the postoperative recurrence rate remains high, and the long-term survival for patients is sub-optimal. In recent years, immunotherapy has garnered extensive attention as an innovative approach to the treatment of gastric cancer. Indeed, multiple studies have validated its therapeutic effects in advanced gastric cancer patients, leading to its incorporation into treatment guidelines. Currently, researchers are exploring the application of immunotherapy in the neoadjuvant setting globally in order to further adjust and refine neoadjuvant immunotherapy regimens for gastric cancer. This article summarizes the research progress and controversies associated with neoadjuvant immunotherapy in gastric cancer, aiming to optimize clinical benefits for gastric cancer patients undergoing this treatment approach. The retrieval methods of this study encompassed databases such as PubMed, Google Scholar, Web of Science, clinicaltrials.gov, etc. The retrieved articles included guidelines, consensus, meta-analyses, clinical trials, and reviews related to locally advanced gastric cancer published up to January 2024.

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ABSTRACT Introduction: Skiing is a sport that demands the high performance of motor coordination with high energy consumption, factors that strongly influence muscle fatigue. Athletes who experience this phenomenon tend to present a decline in performance, generating psychological irritability and impairment in concentration. Diet is closely related to athletic performance, but the proper prescription for skiing athletes still lacks studies. Objective: To explore the methods of recovery from sports fatigue in skiers by providing up-to-date perspectives for effective nutritional intervention during the recovery stage. Methods: Seven young male skiers were selected as research subjects, and caloric intake, among other conditions, was recorded in detail. After three weeks, indices of body weight, body fat rate, serum metabolism, cardiopulmonary capacity, and energy metabolism, among other relevant information, were duly recorded and compared. Results: The intervention did not significantly impact lean body mass indices (p>0.05). The athletes' body weight decreased significantly, and the body fat rate decreased significantly after the nutritional intervention; The analyzed biomarkers were positively impacted, except the urea nitrogen concentration that showed a tendency to increase after the nutritional intervention, an effect that may be linked to the proportional increase of protein intake. Conclusion: The proposed nutritional intervention positively impacted the skiers' energy metabolism, directly impacting the athletes' recovery from sports fatigue. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: O esqui é um esporte que exige alto desempenho de coordenação motora com um elevado consumo de energia, fatores que influenciam fortemente a fadiga muscular. Atletas que experienciam esse fenômeno, tendem a apresentar um declínio no nível do desempenho, gerando irritabilidade psicológica e prejuízos na concentração. A dieta está intimamente relacionada ao desempenho atlético, porém a receita adequada para os atletas em esqui ainda carece de estudos. Objetivo: Explorar os métodos de recuperação da fadiga esportiva nos esquiadores fornecendo perspectivas atualizadas para uma intervenção nutricional eficaz durante a etapa de recuperação. Métodos: Sete jovens esquiadores do sexo masculino foram selecionados como sujeitos da pesquisa, e a ingestão calórica, entre outras condições foram detalhadamente registradas. Após três semanas, índices de peso corporal, taxa de gordura corporal, metabolismo sérico, capacidade cardiopulmonar, metabolismo energético, entre outras informações relevantes foram devidamente registradas e comparadas. Resultados: A intervenção não impactou significativamente nos índices de massa magra corporal (p>0,05). O peso corporal dos atletas diminuiu significativamente e a taxa de gordura corporal diminuiu significativamente após a intervenção nutricional; os biomarcadores analisados foram positivamente impactados, a exceção da concentração de nitrogênio ureico que apresentou tendência de alta após a intervenção nutricional, efeito que pode estar atrelado ao aumento proporcional da ingesta proteica. Conclusão: A proposta de intervenção nutricional apresentou impactos positivos no metabolismo energético dos esquiadores, impactando diretamente na recuperação da fadiga esportiva dos atletas. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: El esquí es un deporte que exige un alto rendimiento de coordinación motora con un elevado consumo de energía, factores que influyen fuertemente en la fatiga muscular. Los deportistas que experimentan este fenómeno, tienden a presentar un descenso en el nivel de rendimiento, generando irritabilidad psicológica y daños en la concentración. La dieta está estrechamente relacionada con el rendimiento deportivo, pero la prescripción adecuada para los atletas de esquí aún carece de estudios. Objetivo: Explorar los métodos de recuperación de la fatiga deportiva en los esquiadores proporcionando perspectivas actualizadas para una intervención nutricional eficaz durante la etapa de recuperación. Métodos: Se seleccionaron siete jóvenes esquiadores varones como sujetos de investigación, y se registró detalladamente la ingesta calórica, entre otras condiciones. Al cabo de tres semanas, se registraron y compararon debidamente los índices de peso corporal, el índice de grasa corporal, el metabolismo sérico, la capacidad cardiopulmonar y el metabolismo energético, entre otros datos relevantes. Resultados: La intervención no tuvo un impacto significativo en los índices de masa corporal magra (p>0,05). El peso corporal de los atletas disminuyó significativamente y el índice de grasa corporal disminuyó significativamente después de la intervención nutricional. Los biomarcadores analizados tuvieron un impacto positivo, con la excepción de la concentración de nitrógeno ureico que mostró una tendencia a aumentar después de la intervención nutricional, efecto que puede estar relacionado con el aumento proporcional de la ingesta de proteínas. Conclusión: La propuesta de intervención nutricional presentó impactos positivos en el metabolismo energético de los esquiadores, impactando directamente en la recuperación de la fatiga deportiva de los atletas. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

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ABSTRACT Introduction: With the preparation and development of the Winter Olympic Games, there has been an increase in the popularity of skiing. Driven by this demand, the professional development of coaches requires updates in the analysis of skiers' sports injuries, elaboration of corresponding prevention and treatment strategies, and resistance training to promote the development of athletes' physical quality, such as bone mass and mineral density. Objective: Study the sports injuries of skiers and explore needs and ways of applying resistance training in skiing. Methods: The questionnaire survey was used to explore the main types of injuries and their influencing factors. Then, 20 volunteers were selected for the resistance training experiment; each group of 10 people included five men and five women. The control group maintained a normal daily life, while resistance training was added to the experimental group three times a week, based on the control group. Results: The research results showed that the current proportion of skiing injuries was relatively high, mainly in mild injuries. After nine weeks in the experimental resistance training group, both men and women had positive bone mass development. Conclusion: Resistance training can improve physical quality and reduce the occurrence of sports injuries, demonstrating the benefits of its implementation in endurance training for skiers. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução: Com a preparação e desenvolvimento dos Jogos Olímpicos de Inverno, houve um aumento da popularidade do esqui. Impulsionados por essa demanda, o desenvolvimento profissional dos treinadores requer atualizações nas análises de lesões esportivas dos esquiadores, elaboração de estratégias de prevenção e tratamento correspondentes, além de treinamentos de resistência de modo a promover o desenvolvimento da qualidade física dos atletas, como a massa óssea e sua densidade mineral. Objetivo: Estudar as lesões esportivas dos esquiadores e explorar necessidades e modos de aplicação do treino de resistência no esqui. Métodos: O método de pesquisa por questionário foi utilizado para explorar os principais tipos de lesões e seus fatores influenciadores. Em seguida, 20 voluntários foram selecionados para o experimento de treinamento de resistência, cada grupo de 10 pessoas incluiu 5 homens e 5 mulheres. O grupo controle manteve uma vida diária normal, enquanto ao grupo experimental foi adicionado um treinamento de resistência, três vezes por semana, com base no grupo controle. Resultados: Os resultados da pesquisa mostraram que a proporção atual de lesões por esqui foi relativamente alta, principalmente em lesões leves. Após 9 semanas no grupo experimental de treinamento de resistência, tanto homens quanto mulheres tiveram desenvolvimento positivo de massa óssea. Conclusão: O treinamento de resistência pode não só melhorar a qualidade física, mas também reduzir a ocorrência de lesões esportivas, demonstrando os benefícios de sua implementação no treinamento de resistência dos esquiadores. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción: Con la preparación y el desarrollo de los Juegos Olímpicos de Invierno, ha aumentado la popularidad del esquí. Impulsado por esta demanda, el desarrollo profesional de los entrenadores requiere actualizaciones en el análisis de las lesiones deportivas de los esquiadores, la elaboración de las correspondientes estrategias de prevención y tratamiento, así como el entrenamiento de la resistencia para promover el desarrollo de la calidad física de los atletas, como la masa ósea y la densidad mineral. Objetivo: Estudiar las lesiones deportivas de los esquiadores y explorar las necesidades y formas de aplicar el entrenamiento de resistencia en el esquí. Métodos: Se utilizó el método de encuesta por cuestionario para explorar los principales tipos de lesiones y sus factores de influencia. A continuación, se seleccionaron 20 voluntarios para el experimento de entrenamiento de resistencia, cada grupo de 10 personas incluía 5 hombres y 5 mujeres. El grupo de control mantuvo una vida diaria normal, mientras que al grupo experimental se le añadió un entrenamiento de resistencia, tres veces por semana, basado en el grupo de control. Resultados: Los resultados de la investigación mostraron que la proporción actual de lesiones de esquí era relativamente alta, principalmente en las lesiones leves. Después de 9 semanas en el grupo experimental de entrenamiento de resistencia, tanto los hombres como las mujeres tuvieron un desarrollo positivo de la masa ósea. Conclusión: El entrenamiento de resistencia no sólo puede mejorar la calidad física, sino también reducir la aparición de lesiones deportivas, lo que demuestra los beneficios de su aplicación en el entrenamiento de resistencia de los esquiadores. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

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ABSTRACT Introduction By improving the material level of life, the amount of exercise of adolescents is decreasing, leading the muscular strength and cardiorespiratory resistance to also decrease instead of increase. Therefore, it is urgent to present effective training methods in physical education teaching to benefit adolescents' quality of life. Objective Study the effect of table tennis training on musculature and cardiorespiratory endurance in young students. Methods 60 students from grade 7 of a high school were randomly divided into experimental and control groups. The experiment lasted for 9 weeks. In this paper, intra-group comparisons and inter-group comparisons were used. Before the experiment and after 9 weeks of training, students had their biophysical indicators collected; these data were analyzed and statistically compared. Results Existing physical education training can overall improve students' physical muscular and cardiopulmonary endurance. The results show that the current training is effective, but inferior to that experienced with table tennis, and should be adjusted. Conclusion In teaching high school youth, physical education teachers are advised to promote table tennis to promote the young student's health. Level of evidence II; Therapeutic studies - investigation of treatment outcomes.

RESUMO Introdução Ao melhorar o nível de vida material, a quantidade de exercício dos adolescentes está diminuindo, levando a força muscular e a resistência cardiorrespiratória a também diminuem ao invés de aumentar. Portanto, é urgente apresentar métodos de treinamento eficazes no ensino da educação física, visando beneficiar o nível de qualidade de vida dos adolescentes. Objetivo Estudar o efeito do treinamento de tênis de mesa sobre a musculatura e a resistência cardiorrespiratória em jovens estudantes. Métodos 60 alunos da série 7 de uma escola de ensino médio foram aleatoriamente divididos em grupo experimental e controle. O experimento durou 9 semanas. Neste trabalho, foram usadas a comparação intragrupo e a comparação intergrupo. Antes do experimento e após 9 semanas de treinamento, os alunos tiveram seus indicadores biofísicos coletados, esses dados foram analisados e comparados estatisticamente. Resultados O treinamento de educação física existentes podem melhorar globalmente a resistência muscular física e cardiopulmonar dos alunos. Os resultados mostram que o treinamento atual é eficaz, porém inferior ao experimentado com o tênis de mesa, devendo ser ajustados. Conclusão Na atual situação do ensino para jovens do ensino médio, é aconselhado aos professores de educação física promoverem o tênis de mesa como uma medida auxiliar na promoção integral da saúde do jovem estudante. Nível de evidência II; Estudos terapêuticos - investigação dos resultados do tratamento.

RESUMEN Introducción Al mejorar el nivel de vida material, la cantidad de ejercicio de los adolescentes está disminuyendo, lo que hace que la fuerza muscular y la resistencia cardiorrespiratoria también disminuyan en lugar de aumentar. Por lo tanto, es urgente presentar métodos de formación eficaces en la enseñanza de la educación física, con el objetivo de beneficiar el nivel de calidad de vida de los adolescentes. Objetivo Estudiar el efecto del entrenamiento de tenis de mesa sobre la musculatura y la resistencia cardiorrespiratoria en jóvenes estudiantes. Métodos 60 estudiantes de 7º curso de un instituto se dividieron aleatoriamente en grupo experimental y grupo de control. El experimento duró 9 semanas. En este trabajo se utilizó la comparación intragrupo y la comparación intergrupo. Antes del experimento y después de 9 semanas de entrenamiento, se recogieron los indicadores biofísicos de los estudiantes, se analizaron estos datos y se compararon estadísticamente. Resultados El entrenamiento de educación física existente puede mejorar la resistencia física muscular y cardiopulmonar general de los estudiantes. Los resultados demuestran que el entrenamiento actual es eficaz, pero inferior al experimentado con el tenis de mesa, y debería ajustarse. Conclusión En la situación actual de la enseñanza para jóvenes de secundaria, se aconseja que los profesores de educación física promuevan el tenis de mesa como medida auxiliar en la promoción integral de la salud del joven estudiante. Nivel de evidencia II; Estudios terapéuticos - investigación de los resultados del tratamiento.

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To reduce the negative impact of nanopesticide carriers of on the environment, a greener nanodelivery system is necessary. Nanogels are nontoxic and degradable carriers, however, the potential of nanogels for delivering pesticides has not been proven. In this study, poly(vinyl alcohol)-valine, an ecofriendly polymer, was synthesized and used to fabricate emamectin benzoate nanogel suspension (EB NS). The nanoformulation showed favorable stability at low temperature, high temperature or one year storage, and in water with different hardnesses. The retention of the EB NS solution on leaves was higher than that of an EB emulsifiable concentrate (EC) by approximately 9% at a concentration of 10 mg L-1. The half-life of EB nanogels under Ultra Violet irradiation was prolonged by 3.3-fold. Moreover, the bioactivity of the EB NS against Plutella xylostella was higher than that of the EB EC. These advantages resulted in a relatively long duration of pest control. The response of nanogels to laccase, a digestive enzyme in the digestive tract of lepidopteran pests, enables pesticide release on demand. Nanogels have the advantages of being ecofriendly carriers, exhibiting higher utilization, and prolonged pest control periods, and they have a brilliant future in pesticide delivery.

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ABSTRACT Introduction: Education administrative departments and schools should regard sports as an integral part of school education and allow students to use sports every day. Guarantee the time for sports activities; schools should organize various forms of extracurricular sports activities". Objective: Based on the ecological system theory, the paper constructs a three-factor structural model that restricts youth sports participation and compiles a scale of factors restricting youth sports participation. Methods: The thesis selected 1,800 primary and secondary school students as the research object and used exploratory factor analysis to explore the questionnaire structure on the obstacles to sports participation. Results: 1) Exploratory factor analysis, the cumulative contribution rate of the first three factors extracted was 58.90%, and the load of each factor was more significant than 0.4. The three factors were school factors, family factors, and personal factors in order; 2) Reselected subjects, Exploring the three-factor structural model of obstacles to sports participation were verified. The final modified model RMSEA was 0.071, CFI was 0.98, and χ2/df was 8.55. The three factors were school factors, family factors, and personal factors. Conclusions: The fit of the three-factor structural indicators restricting youth sports participation is ideal, and the model is relatively stable. The "Questionnaire on Restricting Factors of Youth Sports Participation" can be used as a measurement tool to evaluate and understand youth sports participation constraints. Level of evidence II; Therapeutic studies - investigation of treatment results.

RESUMO Introdução: Departamentos administrativos de educação e escolas devem considerar os esportes como parte integrante da educação escolar e permitir que os alunos pratiquem esportes todos os dias. Garantia de tempo para atividades esportivas; as escolas devem organizar várias formas de atividades esportivas extracurriculares. Objetivo: Com base na teoria do sistema ecológico, o artigo constrói um modelo estrutural de três fatores que restringe a participação em esportes de jovens e compila uma escala de fatores que restringem a participação em esportes de jovens. Métodos: a tese selecionou 1.800 alunos do ensino fundamental e médio como objeto de pesquisa e utilizou uma análise fatorial exploratória para explorar a estrutura do questionário sobre obstáculos à participação esportiva. Resultados: 1) Análise fatorial exploratória, a taxa de contribuição cumulativa dos três primeiros fatores extraídos foi de 58,90% e a carga de cada fator foi mais significativa do que 0,4. Os três fatores eram fatores escolares, fatores familiares e fatores pessoais em ordem; 2) Foram verificados os temas reelecionados, explorando o modelo estrutural de três fatores de obstáculos à participação esportiva. O modelo RMSEA modificado final foi 0,071, CFI foi 0,98 e χ2 / df foi 8,55. Os três fatores eram fatores escolares, fatores familiares e fatores pessoais. Conclusões: O ajuste dos indicadores estruturais de três fatores que restringem a participação dos jovens em esportes é ideal e o modelo é relativamente estável. O "Questionário sobre Fatores Restritivos da Participação em Esportes de Jovens" pode ser usado como uma ferramenta de medição para avaliar e compreender as limitações da participação em esportes de jovens. Nível de evidência II; Estudos terapêuticos: investigação dos resultados do tratamento.

RESUMEN Introducción: Los departamentos administrativos de educación y las escuelas deben considerar los deportes como una parte integral de la educación escolar y permitir que los estudiantes utilicen los deportes todos los días. Garantizar el tiempo para actividades deportivas; las escuelas deberían organizar diversas formas de actividades deportivas extracurriculares. Objetivo: Basado en la teoría del sistema ecológico, el artículo construye un modelo estructural de tres factores que restringe la participación deportiva juvenil y compila una escala de factores que restringen la participación deportiva juvenil. Métodos: La tesis seleccionó a 1.800 estudiantes de primaria y secundaria como objeto de investigación y utilizó un análisis factorial exploratorio para explorar la estructura del cuestionario sobre los obstáculos a la participación deportiva. Resultados: 1) Análisis factorial exploratorio, la tasa de contribución acumulada de los tres primeros factores extraídos fue de 58,90% y la carga de cada factor fue más significativa que 0,4. Los tres factores fueron factores escolares, factores familiares y factores personales en orden; 2) Se verificaron sujetos reselegidos, Explorando el modelo estructural de tres factores de obstáculos a la participación deportiva. El modelo final modificado RMSEA fue 0.071, CFI fue 0.98 y χ2 / df fue 8.55. Los tres factores fueron factores escolares, factores familiares y factores personales. Conclusiones: El ajuste de los indicadores estructurales de tres factores que restringen la participación deportiva juvenil es ideal y el modelo es relativamente estable. El "Cuestionario sobre factores restrictivos de la participación deportiva juvenil" puede utilizarse como una herramienta de medición para evaluar y comprender las limitaciones de la participación deportiva juvenil. Nivel de evidencia II; Estudios terapéuticos: investigación de los resultados del tratamiento.

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Cerebral cavernous malformations (CCMs) form following loss of the CCM protein complex in brain endothelial cells due to increased endothelial MEKK3 signaling and KLF2/4 transcription factor expression, but the downstream events that drive lesion formation remain undefined. Recent studies have revealed that CCM lesions expand by incorporating neighboring wild-type endothelial cells, indicative of a cell nonautonomous mechanism. Here we find that endothelial loss of ADAMTS5 reduced CCM formation in the neonatal mouse model. Conversely, endothelial gain of ADAMTS5 conferred early lesion genesis in the absence of increased KLF2/4 expression and synergized with KRIT1 loss of function to create large malformations. Lowering versican expression reduced CCM burden, indicating that versican is the relevant ADAMTS5 substrate and that lesion formation requires proteolysis but not loss of this extracellular matrix protein. These findings identify endothelial secretion of ADAMTS5 and cleavage of versican as downstream mechanisms of CCM pathogenesis and provide a basis for the participation of wild-type endothelial cells in lesion formation.

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OBJECTIVE: To observe the effects of preoperative right stellate ganglion block on perioperative atrial fibrillation in patients undergoing lung lobectomy. METHODS: Two hundred patients who underwent a scheduled lobectomy were randomly divided into the S and C groups. The S group was injected with 4mL of 0.2% ropivacaine under ultrasound guidance, and the C group did not receive stellate ganglion block. The patients underwent continuous ECG monitoring, and the incidences of atrial fibrillation and other types of arrhythmias were recorded from the start of surgery to 24hours after surgery. RESULTS: The respective incidences of atrial fibrillation in the S group and the C group were 3% and 10% (p=0.045); other atrial arrhythmias were 20% and 38% (p=0.005); and ventricular arrhythmia were 28% and 39% (p=0.09). CONCLUSIONS: The results of the study indicated that preoperative right stellate ganglion block can effectively reduce the incidence of intraoperative and postoperative atrial fibrillation.

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OBJECTIVE: Psychological distress is an important mental health problem among university students. The goal of this study was to determine psychological distress and its associated risk factors among students in the Anhui province. METHODS: A cross-sectional study was conducted in a sample of 1304 students. In this study, a self-administered questionnaire consisting of the general demography and General Health Questionnaire (GHQ-12) was completed. Psychological distress was assessed using the GHQ-12-item questionnaire. A dichotomous category split was imposed on the GHQ-12 for the purpose of analysis. A GHQ-12 score of 4 or higher indicated psychological distress. The data were analyzed by SPSS 20.0 system. RESULTS: A total of 1304 samples were analyzed in this study. The results indicated that the education level of the father and mother was associated with the students' psychological distress (P<0.001). A significant association was found between high-intensity exercise and low-intensity exercise and psychological distress. However, no significant difference was identified between gender and psychological distress (P=0.173). CONCLUSION: The education level of parents, high-intensity exercise, and low-intensity exercise were associated with psychological distress. Our results suggest that it is indispensable to raise awareness of psychological disorders and its associated risk factors among university students. Further studies are required to develop appropriate interventions for high-risk groups.

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Abstract Objective: To observe the effects of preoperative right stellate ganglion block on perioperative atrial fibrillation in patients undergoing lung lobectomy. Methods: Two hundred patients who underwent a scheduled lobectomy were randomly divided into the S and C groups. The S group was injected with 4 mL of 0.2% ropivacaine under ultrasound guidance, and the C group did not receive stellate ganglion block. The patients underwent continuous ECG monitoring, and the incidences of atrial fibrillation and other types of arrhythmias were recorded from the start of surgery to 24 hours after surgery. Results: The respective incidences of atrial fibrillation in the S group and the C group were 3% and 10% (p = 0.045); other atrial arrhythmias were 20% and 38% (p = 0.005); and ventricular arrhythmia were 28% and 39% (p = 0.09). Conclusions: The results of the study indicated that preoperative right stellate ganglion block can effectively reduce the incidence of intraoperative and postoperative atrial fibrillation.

Resumo Objetivo: Observar os efeitos do bloqueio do gânglio estrelado na fibrilação atrial no período perioperatório em pacientes submetidos a lobectomia pulmonar. Método: Duzentos pacientes programados para lobectomia foram divididos aleatoriamente nos grupos S e C. O grupo S recebeu infusão de 4 mL de ropivacaína a 0,2% orientada por ultrassom e o grupo C não foi submetido a bloqueio do gânglio estrelado. Os pacientes foram submetidos à monitoração contínua de ECG, e as incidências de fibrilação atrial e outros tipos de arritmias foram registradas do início da cirurgia até 24 horas depois da cirurgia. Resultados: As incidências de fibrilação atrial no grupo S e no grupo C foram 3% e 10%, respectivamente (p = 0,045); as de outras arritmias atriais foram 20% e 38% (p = 0,005); e de arritmias ventriculares, 28% e 39% (p = 0,09). Conclusões: Os resultados do estudo indicaram que o bloqueio do gânglio estrelado no pré-operatório pode ser efetivo na redução da incidência de fibrilação atrial nos períodos intra- e pós-operatório.

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Herbicide application is an effective weed control method for mitigating crop yield loss; however, herbicide overuse can cause toxicity in non-target plants. The present study evaluated the effects of glufosinate at recommended dose for agricultural application (0.45 kg ha-1) and at overuse dose (0.90 kg ha-1) glufosinate application on photosynthetic performance and nitrogen assimilation of the rapeseed varieties D148 and Zhongshuang 11 (ZS11). Both glufosinate concentrations significantly decreased the content of chlorophyll and nitrogenous compounds, except free proline, and the activity of glutamine synthetase and glutamate synthase, and increased the activity of glutamic acid dehydrogenase in both varieties. When the concentration of glyphosate was 0.45kg ha-1, the nitrogen assimilation of the two varieties decreased, which indicated that the recommended dosage inhibited the nitrogen assimilation of the two varieties; however, the increase of net photosynthetic rate of D148 and the decrease of that of ZS11 mean that D148 is more tolerant to the recommended dose of glyphosate than ZS11. The 0.90 kg ha-1 dosage was toxic to both rapeseed varieties. Overall, our results indicated that herbicide overuse inhibited the photosynthetic rate and nitrogen assimilation in rapeseed seedlings, and it is essential to apply a suitable glufosinate dose based on the variety grown to minimize adverse effects on crops and environment.(AU)

A aplicação de herbicidas é um método eficaz de controle de ervas daninhas para mitigar a perda de produtividade das culturas. No entanto, o uso excessivo de herbicidas pode causar toxicidade em plantas não alvo. O presente estudo avaliou os efeitos da dose recomendada para aplicação agrícola (0.45 kg ha-1) e dose excessiva (0.90 kg ha-1) de glufosinato no desempenho fotossintético e assimilação de nitrogênio das variedades de colza D148 e Zhongshuang 11 (ZS11). Ambas as concentrações de glutamato diminuíram significativamente o teor de clorofila e compostos azotados, exceto a prolina livre, e a atividade de síntese da glutamina e de síntese de glutamato, e aumentaram a atividade de desidrogenase do ácido glutâmico em ambas as variedades. Quando a concentração de glifosato foi 0.45 kg ha-1, a assimilação de azoto das duas variedades diminuiu, o que indicou que a dosagem recomendada de glifosato inibiu a assimilação de azoto das duas variedades de colza. Entretanto, a taxa fotosintética líquida do D148 aumentou enquanto o do ZS11 diminuiu, o que significa que o D148 é mais tolerante a dose recomendada de glifosato do que o ZS11. A dose de 0.90 kg ha-1 de glifosato foi prejudicial para as mudas de duas variedades de colza. Em geral, os nossos resultados indicam que o uso excessivo de glufosinato inibe a taxa fotossintética e a assimilação de nitrogênio em mudas de colza, sendo essencial aplicar uma dose adequada deste herbicida com base na variedade cultivada para minimizar os efeitos adversos nas culturas e no meio ambiente.(AU)

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SUMMARY OBJECTIVE Psychological distress is an important mental health problem among university students. The goal of this study was to determine psychological distress and its associated risk factors among students in the Anhui province. METHODS A cross-sectional study was conducted in a sample of 1304 students. In this study, a self-administered questionnaire consisting of the general demography and General Health Questionnaire (GHQ-12) was completed. Psychological distress was assessed using the GHQ-12-item questionnaire. A dichotomous category split was imposed on the GHQ-12 for the purpose of analysis. A GHQ-12 score of 4 or higher indicated psychological distress. The data were analyzed by SPSS 20.0 system. RESULTS A total of 1304 samples were analyzed in this study. The results indicated that the education level of the father and mother was associated with the students' psychological distress (P<0.001). A significant association was found between high-intensity exercise and low-intensity exercise and psychological distress. However, no significant difference was identified between gender and psychological distress (P=0.173). CONCLUSION The education level of parents, high-intensity exercise, and low-intensity exercise were associated with psychological distress. Our results suggest that it is indispensable to raise awareness of psychological disorders and its associated risk factors among university students. Further studies are required to develop appropriate interventions for high-risk groups.

RESUMO OBJETIVO O estresse psicológico é um importante problema de saúde mental entre estudantes universitários. O objetivo deste estudo foi determinar o estresse psicológico e seus fatores de risco associados entre os estudantes na província de Anhui. MÉTODOS Um estudo transversal foi realizado entre uma amostra de 1.304 indivíduos. Nesse estudo, foi preenchido um questionário autoaplicável que consiste na demografia geral e no Questionário Geral de Saúde (GHQ-12). O problema psicológico foi avaliado utilizando o questionário GHQ de 12 itens. Uma divisão dicotômica de categoria foi imposta ao GHQ-12 para efeito de análise. Uma pontuação de quatro ou mais no GHQ-12 mostrava que um indivíduo tem problemas psicológicos. Os dados foram analisados pelo software SPSS 20.0. RESULTADOS Um total de 1.304 amostras foi analisado neste estudo. Os resultados indicaram que o nível de educação do pai e da mãe estava associado com estudantes em estresse psicológico (P<0,001). Foi encontrada uma associação significativa entre o exercício de alta intensidade e o exercício de baixa intensidade com o estresse psicológico. No entanto, não foi identificada qualquer diferença significativa entre o gênero e o estresse psicológico (P=0,173). CONCLUSÃO O nível de educação dos pais, exercícios de alta intensidade e exercícios de baixa intensidade foram associados com estresse psicológico. Nossos resultados sugerem que é indispensável aumentar a sensibilização para as perturbações psicológicas e para os fatores de risco associados aos estudantes universitários. São necessários mais estudos para desenvolver intervenções adequadas para grupos de alto risco.

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ABSTRACT: Herbicide application is an effective weed control method for mitigating crop yield loss; however, herbicide overuse can cause toxicity in non-target plants. The present study evaluated the effects of glufosinate at recommended dose for agricultural application (0.45 kg ha-1) and at overuse dose (0.90 kg ha-1) glufosinate application on photosynthetic performance and nitrogen assimilation of the rapeseed varieties D148 and Zhongshuang 11 (ZS11). Both glufosinate concentrations significantly decreased the content of chlorophyll and nitrogenous compounds, except free proline, and the activity of glutamine synthetase and glutamate synthase, and increased the activity of glutamic acid dehydrogenase in both varieties. When the concentration of glyphosate was 0.45kg ha-1, the nitrogen assimilation of the two varieties decreased, which indicated that the recommended dosage inhibited the nitrogen assimilation of the two varieties; however, the increase of net photosynthetic rate of D148 and the decrease of that of ZS11 mean that D148 is more tolerant to the recommended dose of glyphosate than ZS11. The 0.90 kg ha-1 dosage was toxic to both rapeseed varieties. Overall, our results indicated that herbicide overuse inhibited the photosynthetic rate and nitrogen assimilation in rapeseed seedlings, and it is essential to apply a suitable glufosinate dose based on the variety grown to minimize adverse effects on crops and environment.

RESUMO: A aplicação de herbicidas é um método eficaz de controle de ervas daninhas para mitigar a perda de produtividade das culturas. No entanto, o uso excessivo de herbicidas pode causar toxicidade em plantas não alvo. O presente estudo avaliou os efeitos da dose recomendada para aplicação agrícola (0.45 kg ha-1) e dose excessiva (0.90 kg ha-1) de glufosinato no desempenho fotossintético e assimilação de nitrogênio das variedades de colza D148 e Zhongshuang 11 (ZS11). Ambas as concentrações de glutamato diminuíram significativamente o teor de clorofila e compostos azotados, exceto a prolina livre, e a atividade de síntese da glutamina e de síntese de glutamato, e aumentaram a atividade de desidrogenase do ácido glutâmico em ambas as variedades. Quando a concentração de glifosato foi 0.45 kg ha-1, a assimilação de azoto das duas variedades diminuiu, o que indicou que a dosagem recomendada de glifosato inibiu a assimilação de azoto das duas variedades de colza. Entretanto, a taxa fotosintética líquida do D148 aumentou enquanto o do ZS11 diminuiu, o que significa que o D148 é mais tolerante a dose recomendada de glifosato do que o ZS11. A dose de 0.90 kg ha-1 de glifosato foi prejudicial para as mudas de duas variedades de colza. Em geral, os nossos resultados indicam que o uso excessivo de glufosinato inibe a taxa fotossintética e a assimilação de nitrogênio em mudas de colza, sendo essencial aplicar uma dose adequada deste herbicida com base na variedade cultivada para minimizar os efeitos adversos nas culturas e no meio ambiente.

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Tagetes erecta is an important ornamental and medicinal plant indigenous to Mexico and Guatemala. The complete chloroplast genome of T. erecta was newly sequenced in this study. The total chloropalst genome size of T. erecta was 152,055 bp. In total, 123 genes were indetified, including 79 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. Twelve genes are containing introns (ycf3 and clpP contained two introns). The overall GC content of this genome was 37.4%. A further phylogenomic analysis of Asteraceae, including 23 taxa, was conducted for the placement of genus Tagetes. The complete plastome of T. erecta will provide a valuable resource for further genetic conservation, evolution, and molecular breading studies in Asteraceae.

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Eleven previously undescribed limonoids, trichisins A-K, including eight structural analogues A-H of trijugin and three H-J mexicanolide derivatives, together with two known mexicanolide derivatives were isolated from the fruits of Heynea trijuga Roxb. ex Sims. The structure determination was based on extensive physical data analyses (NMR, MS), and their basic skeletons and the absolute configurations of trichisins A, B, E, K and trichiconnarone A were assigned via X-ray crystallographic analysis (Cu Kα radiation). The hemiketal motifs in trijugins A, B, and E-G are rare in limonoids. Bioactivity screenings suggested that the trijugin H and mexicanolide-type trichiconnarones A and B limonoids were effective in reversing resistance in MCF-7/DOX cells at a nontoxic concentration of 50 µM with IC50 values of 12.45, 10.86, and 14.96 µM, respectively.

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Maize was domesticated from lowland teosinte (Zea mays ssp. parviglumis), but the contribution of highland teosinte (Zea mays ssp. mexicana, hereafter mexicana) to modern maize is not clear. Here, two genomes for Mo17 (a modern maize inbred) and mexicana are assembled using a meta-assembly strategy after sequencing of 10 lines derived from a maize-teosinte cross. Comparative analyses reveal a high level of diversity between Mo17, B73, and mexicana, including three Mb-size structural rearrangements. The maize spontaneous mutation rate is estimated to be 2.17 × 10-8 ~3.87 × 10-8 per site per generation with a nonrandom distribution across the genome. A higher deleterious mutation rate is observed in the pericentromeric regions, and might be caused by differences in recombination frequency. Over 10% of the maize genome shows evidence of introgression from the mexicana genome, suggesting that mexicana contributed to maize adaptation and improvement. Our data offer a rich resource for constructing the pan-genome of Zea mays and genetic improvement of modern maize varieties.

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Study objective: To assess the extent to which objective sleep patterns vary among U.S. Hispanics/Latinos. Methods: We assessed objective sleep patterns in 2087 participants of the Hispanic Community Health Study/Study of Latinos from 6 Hispanic/Latino subgroups aged 18-64 years who underwent 7 days of wrist actigraphy. Results: The age- and sex-standardized mean (SE) sleep duration was 6.82 (0.05), 6.72 (0.07), 6.61 (0.07), 6.59 (0.06), 6.57 (0.10), and 6.44 (0.09) hr among individuals of Mexican, Cuban, Dominican, Central American, Puerto Rican, and South American heritage, respectively. Sleep maintenance efficiency ranged from 89.2 (0.2)% in Mexicans to 86.5 (0.4)% in Puerto Ricans, while the sleep fragmentation index ranged from 19.7 (0.3)% in Mexicans to 24.2 (0.7)% in Puerto Ricans. In multivariable models adjusted for age, sex, season, socioeconomic status, lifestyle habits, and comorbidities, these differences persisted. Conclusions: There are important differences in actigraphically measured sleep across U.S. Hispanic/Latino heritages. Individuals of Mexican heritage have longer and more consolidated sleep, while those of Puerto Rican heritage have shorter and more fragmented sleep. These differences may have clinically important effects on health outcomes.

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