RESUMEN
This work aimed to investigate the hypoglycemic effects and underlying mechanism of whole grain proso millet (Panicum miliaceum L.; WPM) on type 2 diabetes mellitus (T2DM). The results showed that WPM supplementation significantly reduced fasting blood glucose (FBG) and serum lipid levels in T2DM mice induced by a high-fat diet (HFD) combined with streptozotocin (STZ), with improved glucose tolerance, liver and kidney injury, and insulin resistance. In addition, WPM significantly inhibited the expression of gluconeogenesis-related genes G6pase, Pepck, Foxo1, and Pgc-1α. Further study by miRNA high-throughput sequencing revealed that WPM supplementation mainly altered the liver miRNA expression profile of T2DM mice by increasing the expression of miR-144-3p_R-1 and miR-423-5p, reducing the expression of miR-22-5p_R-1 and miR-30a-3p. GO and KEGG analyses showed that the target genes of these miRNAs were mainly enriched in the PI3K/AKT signaling pathway. WPM supplementation significantly increased the level of PI3K, p-AKT, and GSK3ß in the liver of T2DM mice. Taken together, WPM exerts antidiabetic effects by improving the miRNA profile and activating the PI3K/AKT signaling pathway to inhibit gluconeogenesis. This study implies that PM can act as a dietary supplement to attenuate T2DM.
Asunto(s)
Diabetes Mellitus Experimental , Diabetes Mellitus Tipo 2 , Hiperglucemia , MicroARNs , Panicum , Ratones , Animales , Panicum/metabolismo , Diabetes Mellitus Tipo 2/tratamiento farmacológico , Diabetes Mellitus Tipo 2/genética , Diabetes Mellitus Tipo 2/metabolismo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Diabetes Mellitus Experimental/tratamiento farmacológico , Diabetes Mellitus Experimental/genética , Diabetes Mellitus Experimental/metabolismo , Fosfatidilinositol 3-Quinasas/metabolismo , Granos Enteros , Hiperglucemia/tratamiento farmacológico , Hiperglucemia/genética , Hiperglucemia/metabolismo , Hígado/metabolismo , MicroARNs/genética , MicroARNs/metabolismo , Hipoglucemiantes/metabolismoRESUMEN
In this paper, a band-stop filter based on a surface plasmon polariton metal-insulator-metal is designed and studied. The relationship between wavelength and filter transmittance is simulated using the finite difference time domain method and coupled mode theory. Compared with a single-diamond resonator, the minimum transmittances of the double-diamond resonator and double-rectangular resonator at a fixed wavelength are increased by 11.33% and 14.25%, respectively, achieving an enhancement effect. The research results also show that the sensitivity of the filter can reach 860 nm/RIU. The structure has good application prospects in optical integration, optical communication, and optical information processing.
RESUMEN
A dual broadband terahertz bifunction absorber that can be actively tuned is proposed. The optical properties of the absorber were simulated and numerically calculated using the finite-difference time-domain (FDTD) method. The results show that when the conductivity of vanadium dioxide is less than σ0=8.5×103 S/m, the absorptance can be continuously adjusted between 2% and 100%. At vanadium dioxide conductivity greater than σ0=8.5×103 S/m, the absorption bandwidth of the absorber can be switched from 3.4 THz and 3.06 THz to 2.83 THz and none, respectively, and the absorptance remains above 90%. This achieves perfect modulation of the absorptance and absorption bandwidth. The physical mechanism of dual-broadband absorptions and perfect absorption is elucidated by impedance matching theory and electric field distribution. In addition, it also has the advantage of being polarization insensitive and maintaining stable absorption at wide angles of oblique incidence. The absorber may have applications in emerging fields such as modulators, stealth and light-guided optical switches.
RESUMEN
Oregano essential oil (OEO) is an effective natural antibacterial agent, but its antibacterial activity against Vibrio vulnificus has not been widely studied. The aim of this study was to investigate the inhibitory effect and germicidal activity of OEO on V. vulnificus and its possible inhibition mechanism. The minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of OEO against four V. vulnificus strains (ATCC 27562 and three isolates from seafoods) were from 0.06 to 0.15 µL/mL. Compared with untreated bacteria, OEO reduced the concentration of intracellular adenosine triphosphate (ATP), hyperpolarized the cell membrane, increased the level of reactive oxygen species (ROS), and increased the concentration of intracellular malondialdehyde (MDA), but there was no obvious DNA damage at the OEO test concentration. It was indicated that OEO inactivated V. vulnificus by generating ROS which caused lipid peroxidation of cell membranes, thereby reducing the permeability and integrity of cell membranes and causing morphological changes to cells, but there was no obvious damage to DNA. In addition, OEO could effectively kill V. vulnificus in oysters at 25 °C, and the number of bacteria decreased by 48.2% after 0.09% OEO treatment for 10 h. The good inhibitory effect and bactericidal activity of OEO showed in this study, and the economy and security of OEO make it possible to apply OEO to control V. vulnificus contamination in oysters and other seafoods.