RESUMEN
OBJECTIVE: To investigate the protective effects of the integrated traditional Chinese and western medicine Xuebijing injection on stress-induced organ damage in rabbits. METHODS: Forty rabbits were randomly divided into four groups: the control group, the model group, the western medicine group, and the Xuebijing group. The stress-induced organ damage model was replicated by soak the rabbits in water, the animals in the western medicine group and Xuebijing group received injection of lytic cocktail and Xuebijing, respectively. The changes in cortisol (Cor), thromboxane A(2) (TXA(2)), endothelin (ET), nitric oxide synthase (NOS) were determined at different time points in all the groups. The pathologic changes of the gastric mucosa, the adrenal gland and the cardiac muscle cell were observed. RESULTS: The content of Cor increased significantly in model group (P<0.01). The content of Cor decreased in the western medicine group and Xuebijing group, the changes showed no significant difference between two groups (P>0.05). The contents of ET, TXA(2) decreased and NOS increased in Xuebijing group compared with the western medicine group, the differences were significant (all P<0.05). The pathological changes of the gastric mucosa, the adrenal gland and the cardiac myocyte were less marked in Xuebijing group, compared with the western medicine group, the difference was significant (P<0.05). CONCLUSION: Xuebijing has better protective effects on stress-induced organ damage.
Asunto(s)
Glándulas Suprarrenales/patología , Medicamentos Herbarios Chinos/farmacología , Mucosa Gástrica/patología , Miocitos Cardíacos/patología , Estrés Fisiológico , Glándulas Suprarrenales/efectos de los fármacos , Animales , Modelos Animales de Enfermedad , Endotelinas/sangre , Mucosa Gástrica/efectos de los fármacos , Hidrocortisona/sangre , Miocitos Cardíacos/efectos de los fármacos , Óxido Nítrico Sintasa/sangre , Conejos , Distribución Aleatoria , Tromboxano A2/sangreRESUMEN
OBJECTIVE: To investigate the effects of Xuebijing injection on multiple organ dysfunction and mortality in rats with delayed resuscitation after scald injury. METHODS: Wistar rats were subjected to a 30% total body surface area (TBSA) full-thickness scald injury in the experimental groups. One hundred and thirty male Wistar rats were randomly divided into sham scald group (n=10), thermal injury group (n=60, 40 ml/kg normal saline was infused peritoneally 6 hours postburn), and Xuebijing treatment group (n=60, 4 ml/kg Xuebijing injection was injected twice a day in addition to delayed resuscitation). Animals of the thermal injury group and Xuebijing treatment group were re-divided into three subgroups according to the different time points of treatment: 2 hours before scald (n=20), 2 hours after scald (n=20), and 12 hours after scald (n=20). Multiple organ function parameters and 7-day mortality were determined in all the groups. RESULTS: The mortality of animals with Xuebijing treatment at 12 hours postburn was significantly lower than that of scald controls (75.0% vs. 40.0%, P<0.05). In comparison with scald group, the animals in Xuebijing treatment group were found to have significantly decreased values of organ functional parameters at 12 hours postburn, including serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), creatinine (Cr) and creatine kinase (CK, P<0.05 or P<0.01). CONCLUSION: These findings indicate that Xuebijing injection could obviously lower the mortality of rats with delayed resuscitation after burn injury, and it markedly protects against multiple organ dysfunction secondary to severe burns.