RESUMEN
Fishbone is the most common ingested gastrointestinal foreign matter and is less than 1% perforate. However, a fishbone penetrating the gastrointestinal tract and causing granulomatous inflammation of the greater omentum with local suppuration is not common. Because of the nonspecific clinical symptoms, gastrointestinal perforation may be manifested only as dull abdominal pain, which is often ignored and timely clinical treatment may be delayed. We report a case of a 61-year male who experienced intermittent right median ventral abdominal pain for half a year. These symptoms were the result of granulomatous inflammation of the greater omentum with local suppuration caused by a migrating fishbone (3.5 cm in length). Finally, the fishbone was removed by exploratory laparotomy. Key Words: Fishbone, Gastrointestinal perforation, Greater omentum, Granulomatous inflammation, Laparotomy.
Asunto(s)
Cuerpos Extraños , Epiplón , Dolor Abdominal/etiología , Cuerpos Extraños/complicaciones , Cuerpos Extraños/cirugía , Humanos , Inflamación/complicaciones , Masculino , Supuración/complicacionesRESUMEN
OBJECTIVE: To investigate the expression of miR-22-3p in breast cancer and the mechanism of targeting PLAGL2 to inhibit the invasion and migration in human breast cancer. STUDY DESIGN: An experimental study. PLACE AND DURATION OF STUDY: Department of Oncology and Department of General Surgery, The People's Hospital of China Three Gorges University, China, from March 2019 to December 2020. METHODOLOGY: The miR-22-3p expression level in 41 paired human primary breast invasive ductal carcinoma tissues and para-cancer tissues was obtained by real-time fluorescence quantitative reverse transcriptase PCR (qRT-PCR). The effect of miR-22-3p on the proliferation of breast cancer cells was detected by growth curve method. Online software TargetScan was used to predict the target genes of miR-22-3p. The prediction results were verified by luciferase reporter gene assay and qRTâPCR. RESULTS: MiR-22-3p expression was significantly decreased in the breast cancer tissues than in paraâcarcinoma normal breast tissues (p<0.05). Over-expression of miR-22-3p can inhibit the proliferation of MCF-7 cells significantly. Pleomorphic adenoma gene-like protein 2(PLAGL2) is the predicted target gene of miR-22-3p. MiR-22-3p binds to its predicted target gene PLAGL2-3'UTR. The expression of miR-22-3p was negatively correlated with PLAGL2 in MCF-7 cells. CONCLUSION: MiR-22-3p could suppress the proliferation of breast cancer by targeting PLAGL2. This suggests that miR-22-3p may be a strategy of choice for targeted therapy of breast cancer. Key Words: Breast cancer, MiR-22-3p, PLAGL2, Cell proliferation.
Asunto(s)
Neoplasias de la Mama , MicroARNs , Neoplasias de la Mama/genética , Línea Celular Tumoral , Movimiento Celular , Proliferación Celular , China , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Femenino , Regulación Neoplásica de la Expresión Génica , Humanos , MicroARNs/genética , Proteínas de Unión al ARN/genética , Factores de Transcripción/genéticaRESUMEN
Merkel cell carcinoma (MCC) is a rare aggressive cutaneous neuroendocrine carcinoma that typically arises in sunexposed areas of the skin. Herein, we present three cases of primary MCC of the cheek. Case 1 had multiple nodules and mild swelling on the left cheek and eyelids, the patient did not receive any treatment and died after 10 months. Case 2 presented with nodule on patient's left cheek, which increased gradually. The patient was treated with surgical resection from the tumor edge 0.3 cm and skin flap transplantation, she died after 21 months. Case 3 presented with a nodule without any symptoms on patient's left cheek, she was treated with surgical resection and the left cheek was reconstructed with a free flap of tissue. Outpatient follow-up did not show any recurrence and metastasis. The authors report the clinical, histopathological, therapy, prognosis features of MCC, and reviewed with literature.
Asunto(s)
Carcinoma de Células de Merkel/patología , Mejilla/patología , Neoplasias Cutáneas/patología , Anciano , Anciano de 80 o más Años , Carcinoma de Células de Merkel/cirugía , Femenino , Humanos , Inmunoquímica , Masculino , Resultado del TratamientoRESUMEN
A pot experiment was conducted in a greenhouse on one-year-old clonal seedlings of Cunninghamia lanceolata. Five treatments were designed including control, conventional fertilization and three exponential fertilization treatments, with N application rates of 0, 0.5, 0.5, 1.0 and 2.0 g·seedling-1, with N applied at 20 times at 10-day interval. The height, ground diameter, biomass, chlorophyll fluorescence and N absorption were measured after 210 days of N application. The results showed that fertilization significantly promoted the seedling height, ground diameter and biomass with the optimal values of 59.0 cm, 6.0 mm, 52.99 g·seedling-1, in exponential fertilization treatment of 1.0 g N·seedling-1. Chlorophyll fluorescence in exponential fertilization treatments was significantly higher than that in conventional fertilization. The photochemical quenching, PS2 electron transport rate and photochemical efficiency of PS2 in exponential fertilization treatments increased first and then decreased, with the optimal values of 0.89, 35.79 and 0.71 in 1.0 g N·seedling-1 treatment. N contents in different organs of C. lanceolata clonal seedlings were in the order of leaf > root > stem. Compared with the CK, the N contents in root, stem and leaf increased by 39.6%, 16.6% and 41.1% in the conventional fertilization treatment, and by 22.6%-81.4%, 27.3%-152.6% and 73.6%-135.5% in exponential fertilization treatments. N contents in root, stem and leaf in the exponential fertilization treatments of 1.0 and 2.0 g N·seedling-1 were significantly higher than those in the conventional fertilization treatment. P and K contents were not significantly different among the different organs. Considering the characteristics of seedling growth, chlorophyll fluorescence, the contents of N, P and K, the exponential fertilization treatment of 1.0g N· seedling-1 was the best in cultivating C. lanceolata clonal seedlings in this experiment.