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1.
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;46(6): 502-506, 02/jul. 2013. tab, graf
Artículo en Inglés | LILACS | ID: lil-679204

RESUMEN

Single nucleotide polymorphisms in the promoter region of interleukin-18 (IL-18), an inflammatory cytokine, have been linked to susceptibility to many diseases, including cancer and immune dysfunction. Here, we explored the potential association between the IL-18 -607C/A (rs1946518) promoter region polymorphism and susceptibility to ischemic stroke (IS). This locus was amplified from peripheral blood samples of 386 IS patients (cases) and 364 healthy individuals (controls) by the polymerase chain reaction with sequence-specific primers. Significant differences were observed by the χ2 test in the -607C/A (rs1946518) genotype and allele frequencies between cases and controls (P < 0.05). Furthermore, after excluding for age, gender, smoking status, and hypertension, logistic regression indicated that IS susceptibility of -607C carriers increased 1.6 times (OR = 1.601, 95%CI = 1.148-2.233, P = 0.006) compared to -607A carriers. Additionally, similar increases in IS risk were noted for male patients or patients less than 65 years old. In conclusion, IL-18 -607C/A (rs1946518) promoter polymorphism is associated with IS susceptibility, and the C allele may confer increased IS risk.


Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Isquemia Encefálica/genética , /genética , Polimorfismo de Nucleótido Simple , Regiones Promotoras Genéticas/genética , Accidente Cerebrovascular/genética , Isquemia Encefálica/epidemiología , Genotipo , Predisposición Genética a la Enfermedad/epidemiología , Reacción en Cadena de la Polimerasa , Estudios Prospectivos , Fumar , Accidente Cerebrovascular/epidemiología
2.
Braz J Med Biol Res ; 46(6): 502-6, 2013 06.
Artículo en Inglés | MEDLINE | ID: mdl-23739747

RESUMEN

Single nucleotide polymorphisms in the promoter region of interleukin-18 (IL-18), an inflammatory cytokine, have been linked to susceptibility to many diseases, including cancer and immune dysfunction. Here, we explored the potential association between the IL-18 -607C/A (rs1946518) promoter region polymorphism and susceptibility to ischemic stroke (IS). This locus was amplified from peripheral blood samples of 386 IS patients (cases) and 364 healthy individuals (controls) by the polymerase chain reaction with sequence-specific primers. Significant differences were observed by the χ2 test in the -607C/A (rs1946518) genotype and allele frequencies between cases and controls (P < 0.05). Furthermore, after excluding for age, gender, smoking status, and hypertension, logistic regression indicated that IS susceptibility of -607C carriers increased 1.6 times (OR = 1.601, 95%CI = 1.148-2.233, P = 0.006) compared to -607A carriers. Additionally, similar increases in IS risk were noted for male patients or patients less than 65 years old. In conclusion, IL-18 -607C/A (rs1946518) promoter polymorphism is associated with IS susceptibility, and the C allele may confer increased IS risk.


Asunto(s)
Isquemia Encefálica/genética , Interleucina-18/genética , Polimorfismo de Nucleótido Simple , Regiones Promotoras Genéticas/genética , Accidente Cerebrovascular/genética , Adulto , Anciano , Isquemia Encefálica/epidemiología , Femenino , Predisposición Genética a la Enfermedad/epidemiología , Genotipo , Humanos , Masculino , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa , Estudios Prospectivos , Fumar , Accidente Cerebrovascular/epidemiología
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