RESUMEN
Ocular lesions are common in red-tailed hawks with West Nile (WN) disease. These lesions consist of pectenitis, choroidal or retinal inflammation, or retinal necrosis, but detailed investigation of the ocular lesions is lacking. Postmortem examination of the eyes of 16 red-tailed hawks with naturally acquired WN disease and 3 red-tailed hawks without WN disease was performed using histopathology, immunohistochemistry for West Nile virus (WNV) antigen, glial fibrillary acid protein, cleaved caspase-3, and the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling method. Retinal lesions were classified as type I or type II lesions. Type I lesions were characterized by lymphoplasmacytic infiltrates in the subjacent choroid with degeneration limited to the outer retina (type Ia lesion) or with degeneration and necrosis of the outer retina or outer and inner retina (type Ib lesion) while retinal collapse, atrophy, and scarring were hallmarks of type II lesions. Type II retinal lesions were associated with a more pronounced choroiditis. Although not statistically significant, WNV antigen tended to be present in larger quantity in type Ib lesions. Type I lesions are considered acute while type II lesions are chronic. The development of retinal lesions was associated with the presence of an inflammatory infiltrate in the choroid. A breakdown of the blood-retina barrier is suspected to be the main route of infection of the retina. Within the retina, virus appeared to spread via both neuronal and Müller cell processes.
Asunto(s)
Enfermedades de las Aves/virología , Oftalmopatías/veterinaria , Halcones , Fiebre del Nilo Occidental/veterinaria , Animales , Enfermedades de las Aves/epidemiología , Enfermedades de las Aves/patología , Oftalmopatías/epidemiología , Oftalmopatías/patología , Oftalmopatías/virología , Minnesota/epidemiología , Fiebre del Nilo Occidental/epidemiología , Fiebre del Nilo Occidental/patologíaRESUMEN
Pulmonary atresia with intact ventricular septum, rudimentary tricuspid valve, hypoplastic right ventricle, and right-to-left atrial shunting were identified in a four-day-old, male Arabian foal with clinical signs of cyanotic heart disease. Pulmonary blood flow was apparently derived from a ductus arteriosus. Echocardiographic evaluation revealed the majority of cardiac abnormalities and also findings compatible with right-sided congestive heart failure. Congenital cardiac defects have a high incidence in this breed, and this is the first description of this combination of congenital cardiac defects.
Asunto(s)
Cardiopatías Congénitas/veterinaria , Ventrículos Cardíacos/anomalías , Caballos/anomalías , Atresia Pulmonar/veterinaria , Animales , Animales Recién Nacidos , Ecocardiografía/veterinaria , Cardiopatías Congénitas/patología , Ventrículos Cardíacos/patología , Enfermedades de los Caballos/patología , Masculino , Atresia Pulmonar/patologíaRESUMEN
BACKGROUND: A poorly understood protein-losing enteropathy (PLE) disorder has been reported in Yorkshire Terrier dogs. OBJECTIVES: To describe clinical features, intestinal histopathology, and outcome in Yorkshire Terrier dogs with PLE, and to identify variables predictive of outcome. ANIMALS: Thirty client-owned Yorkshire Terrier dogs with PLE. METHODS: Retrospective study. Records of dogs with a diagnosis of PLE were reviewed. Intestinal histopathology was interpreted using the World Small Animal Veterinary Association gastrointestinal histopathology classification system. Discriminate analysis techniques were used to identify variables predictive of outcome. RESULTS: Females outnumbered males (20/30). Median age was 7 years (range 1-12). Common clinical signs were diarrhea (20/30), vomiting (11), ascites and abdominal distension (11), and respiratory difficulty (8). Histopathologic abnormalities included villous lymphatic dilatation, crypt lesions, villous stunting, and variable increases in cellularity of the lamina propria. All dogs were treated with glucocorticoids. Of 23 dogs with long-term follow-up, 9 had complete, and 3 had partial, resolution of signs, and 11 failed to respond to treatment. Median survival of responders was 44 months and of nonresponders was 12 months, with 4 dogs experiencing peracute death. Vomiting, monocytosis, severity of hypoalbuminemia, low blood urea nitrogen concentration, and villous blunting were predictive of survival <4 months. CONCLUSIONS: In addition to classic GI signs, Yorkshire Terriers with PLE often show clinical signs associated with hypoalbuminemia and low oncotic pressure. Lymphatic dilatation, crypt lesions, and villous stunting are consistent histopathologic findings. Clinical outcomes are variable, but many dogs experience remission of clinical signs and prolonged survival.
Asunto(s)
Enfermedades de los Perros/patología , Intestinos/patología , Enteropatías Perdedoras de Proteínas/veterinaria , Animales , Enfermedades de los Perros/diagnóstico , Enfermedades de los Perros/tratamiento farmacológico , Perros , Duodeno/patología , Femenino , Inmunosupresores/uso terapéutico , Masculino , Enteropatías Perdedoras de Proteínas/diagnóstico , Enteropatías Perdedoras de Proteínas/tratamiento farmacológico , Enteropatías Perdedoras de Proteínas/patología , Estudios RetrospectivosRESUMEN
Three young domestic shorthair cats were presented for necropsy with similar histories of slowly progressive visual dysfunction and neurologic deficits. Macroscopic examination of each cat revealed cerebral and cerebellar atrophy, dilated lateral ventricles, and slight brown discoloration of the gray matter. Histologically, there was bilateral loss of neurons within the limbic, motor, somatosensory, visual, and, to a lesser extent, vestibular systems with extensive astrogliosis in the affected regions of all 3 cases. Many remaining neurons and glial cells throughout the entire central nervous system were distended by pale yellow to eosinophilic, autofluorescent cytoplasmic inclusions with ultrastructural appearances typical of neuronal ceroid-lipofuscinoses (NCLs). Differences in clinical presentation and neurological lesions suggest that the 3 cats may have had different variants of NCL. Molecular genetic characterization in the 1 cat from which DNA was available did not reveal any plausible disease-causing mutations of the CLN1 (PPT1), CLN3, CLN5, CLN8, and CLN10 (CTSD) genes. Further investigations will be required to identify the mutations responsible for NCLs in cats.
Asunto(s)
Enfermedades de los Gatos/patología , Sistema Nervioso/patología , Lipofuscinosis Ceroideas Neuronales/veterinaria , Animales , Atrofia/patología , Atrofia/veterinaria , Gatos , Análisis Mutacional de ADN/veterinaria , Resultado Fatal , Técnicas Histológicas/veterinaria , Inmunohistoquímica/veterinaria , Minnesota , Lipofuscinosis Ceroideas Neuronales/patologíaRESUMEN
Two Boxer dogs developed progressive ataxia in association with a neoplastic infiltration of the spinal leptomeninges. In the first dog, the leptomeningeal neoplasm encompassed the entire cord and the ventral aspect of the brainstem and extended bilaterally into the piriform lobes. In the second, the neoplasm surrounded the C1-C3 segments of the spinal cord and the brainstem without involvement of the brain or spinal cord parenchyma. In both dogs, the neoplastic cells had variably distinct cell borders, clear to eosinophilic cytoplasm, and a round to ovoid hyperchromatic nucleus. Neoplastic cells were immunopositive for Olig2 and doublecortin in both dogs and for vimentin in one dog but were immunonegative for glial fibrillary acidic protein, S-100, CD34, E-cadherin, cytokeratin, CD3, and CD20. The morphological and immunohistochemical features of the neoplastic cells were consistent with an oligodendrocyte lineage. This hitherto poorly recognized neoplasm in dogs is analogous to human leptomeningeal oligodendrogliomatosis.
Asunto(s)
Enfermedades de los Perros/patología , Neoplasias Meníngeas/veterinaria , Oligodendroglioma/veterinaria , Animales , Perros , Proteínas de Dominio Doblecortina , Resultado Fatal , Femenino , Inmunohistoquímica/veterinaria , Imagen por Resonancia Magnética/veterinaria , Masculino , Neoplasias Meníngeas/patología , Proteínas Asociadas a Microtúbulos/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Neuropéptidos/metabolismo , Oligodendroglioma/patologíaRESUMEN
Two wild fledgling kestrels exhibited lack of motor coordination, postural reaction deficits, and abnormal propioception. At necropsy, the cerebellum and brainstem were markedly underdeveloped. Microscopically, there was Purkinje cells heterotopy, abnormal circuitry, and hypoplasia with defective foliation. Heterotopic neurons were identified as immature Purkinje cells by their size, location, immunoreactivity for calbindin D-28 K, and ultrastructural features. The authors suggest that this cerebellar abnormality was likely due to a disruption of molecular mechanisms that dictate Purkinje cell migration, placement, and maturation in early embryonic development. The etiology of this condition remains undetermined. Congenital central nervous system disorders have rarely been reported in birds.
Asunto(s)
Enfermedades de las Aves/patología , Cerebelo/anomalías , Cerebelo/patología , Falconiformes , Malformaciones del Sistema Nervioso/veterinaria , Células de Purkinje/patología , Animales , Tronco Encefálico/patología , Movimiento Celular , Cerebelo/ultraestructura , Discapacidades del Desarrollo/patología , Femenino , Masculino , Malformaciones del Sistema Nervioso/patología , Embarazo , Células de Purkinje/ultraestructuraRESUMEN
Tissue cysts of a protozoan parasite were present in the skeletal muscle of a domestic pigeon (Columba livia f. domestica) with neurologic disease in Minnesota, USA. The animal had a severe granulomatous meningoencephalitis. The cysts were slender, up to 1 mm long and up to 0.03 mm in diameter. The cysts had a smooth wall without projections. Size and wall morphology were compatible with Sarcocystis calchasi. Polymerase chain reaction using S. calchasi-specific primers resulted in a specific amplicon from the skeletal muscle but not from the brain. Sequencing of the highly variable genomic regions ITS1 and D2 revealed 100% nucleic acid identity with the German strain of S. calchasi. Sarcocystis calchasi is the cause of an emerging lethal disease in pigeons in Germany. This is the first description of the parasite outside of Germany.
Asunto(s)
Enfermedades del Sistema Nervioso Central/veterinaria , Columbidae , Sarcocystis/clasificación , Sarcocistosis/veterinaria , Animales , Enfermedades del Sistema Nervioso Central/epidemiología , Enfermedades del Sistema Nervioso Central/parasitología , Masculino , Minnesota/epidemiología , Sarcocistosis/epidemiología , Sarcocistosis/parasitologíaRESUMEN
The 2008 case presented here of tularaemia in a cat and its owner occurred in an urban setting and was associated with animal contact, a relatively rare mode of transmission in Minnesota in recent years. Response to this case exemplified a 'One Health' approach involving pre-existing relationships, cooperation between multiple disciplines and laboratory infrastructure that facilitated information sharing.
Asunto(s)
Francisella tularensis/aislamiento & purificación , Tularemia/diagnóstico , Animales , Autopsia , Mordeduras y Picaduras , Gatos , Ciprofloxacina/administración & dosificación , Trazado de Contacto , Doxiciclina/administración & dosificación , Femenino , Francisella tularensis/genética , Humanos , Infusiones Intravenosas , Masculino , Minnesota , Mascotas/microbiología , Resultado del Tratamiento , Tularemia/tratamiento farmacológico , Tularemia/microbiología , Tularemia/transmisión , Tularemia/veterinariaRESUMEN
BACKGROUND: A description of the clinical signs and necropsy findings in 10 alpacas with thrombotic endocarditis. ANIMALS: Clinical cases admitted to 2 veterinary referral hospitals between May 1998 and December 2006. METHODS: A retrospective study was performed by searching hospital records to identify alpacas diagnosed with endocarditis. RESULTS: Common clinical findings included sternal recumbency, tachycardia, tachypnea, and abdominal distension. Heart sounds were recorded as normal in 7 of 10 alpacas. Pleural and pericardial effusion and ascites were often present. Complete blood cell counts often suggested inflammation, and liver enzyme activity was often increased. When echocardiography was performed, a soft tissue density was imaged within the right ventricle. All alpacas died or were euthanized. Necropsy revealed mural endocarditis with right ventricular or biventricular fibrinous thrombi obliterating the ventricular lumina with no valvular involvement in 6 of 10 affected animals. Bacteria were not consistently identified as a cause for the endocarditic lesions. Eight of the 10 alpacas had evidence of hepatic fluke infestation. CONCLUSIONS AND CLINICAL IMPORTANCE: Valvular and mural thrombotic endocarditis should be included in the list of differential diagnoses for hepatomegaly, abdominal distension, and other signs of right-sided congestive heart failure in alpacas. The prognosis of this disease is grave.
Asunto(s)
Camélidos del Nuevo Mundo , Endocarditis/veterinaria , Trombosis/veterinaria , Animales , Análisis Químico de la Sangre/veterinaria , Endocarditis/sangre , Endocarditis/patología , Femenino , Masculino , Estudios Retrospectivos , Trombosis/sangre , Trombosis/patologíaRESUMEN
A neuronal storage disease affecting 5 captive Humboldt penguins is described. One bird died after 3 days of lethargy and anorexia. The 4 remaining birds died after a slowly progressing course of disease with signs that included lethargy, weakness, and neurologic dysfunction. Neurologic signs included dysphagia and ataxia. Gross lesions in the first animal to die consisted of hepatosplenomegaly indicative of avian malaria, which was confirmed histologically. The 4 remaining animals were mildly to moderately emaciated. Moderate to marked vacuolation of the neuronal perikarya was observed in Purkinje cells, neurons of the brainstem nuclei, and motorneurons of the spinal cord in all birds. By electron microscopy the vacuoles represented multilayered concentric lamellar structures. These findings were indicative of sphingolipidosis. All animals had been prophylactically treated for avian malaria, aspergillosis, and possible bacterial infections with chloroquine, itraconazole, and enrofloxacin. Circumstantial evidence implicates chloroquine therapy as the possible cause of the storage disease.
Asunto(s)
Enfermedades de las Aves/patología , Enfermedades por Almacenamiento Lisosomal del Sistema Nervioso/veterinaria , Spheniscidae , Animales , Animales de Zoológico , Enfermedades de las Aves/genética , Tronco Encefálico/patología , Femenino , Enfermedades por Almacenamiento Lisosomal del Sistema Nervioso/genética , Neuronas/patología , Linaje , Médula Espinal/patologíaRESUMEN
In the present study, an outbreak of proliferative dermatitis in musk ox (Ovibos moschatus), Sichuan takin (Budorcas taxicolor tibetana) and domestic Shetland sheep (Ovis aries) in a zoo is described. Skin lesions consisted of severe, persistent, multifocal, proliferative dermatitis in musk ox, and mild, transient, focal, dermatitis in the Sichuan takin and Shetland sheep. Parapoxviruses were isolated from skin lesions, and characterized by restriction enzyme analysis and partial gene sequencing. The results of this investigation indicate that the outbreak of proliferative dermatitis was due to infection by a single parapoxvirus, which is genetically closely related to other orf virus (ORFV) strains but distant to bovine papular stomatitis virus (BPSV) and pseudocowpox virus (PCPV).
Asunto(s)
Animales de Zoológico/virología , Ectima Contagioso/virología , Virus del Orf/genética , Rumiantes/virología , Secuencia de Aminoácidos , Animales , Secuencia de Bases , ADN Viral/química , ADN Viral/genética , Femenino , Masculino , Datos de Secuencia Molecular , Virus del Orf/crecimiento & desarrollo , Reacción en Cadena de la Polimerasa/veterinaria , Mapeo Restrictivo/veterinaria , Alineación de Secuencia , Piel/ultraestructura , Piel/virología , Proteínas del Envoltorio Viral/química , Proteínas del Envoltorio Viral/genéticaRESUMEN
In the present study the occurrence of morbillivirus infection in harbour porpoises (Phocoena phocoena) from the Black Sea was investigated. Blood and tissue specimens of lung, brain and spleen from 73 stranded or by-caught harbour porpoises derived from the three Black Sea subregions such as Bulgaria, Georgia and Ukraine were collected between 1997 and 1999 and processed for histology, immunohistochemistry and serology. Age determination was performed according to dental growth layers and body length. The age of the investigated population ranged from neonates to a 12-year-old animal. Morbillivirus-specific neutralizing antibodies were detected in 53% of harbour porpoises. Generally, titres were very low and ranged from 20 to 270. There was no correlation between age, geographical origin and titre levels. The most common histological finding (97%) consisted of a mild to severe granulomatous bronchopneumonia due to lung worm infection. There were no changes indicative of a morbillivirus infection. Using immunohistology none of the animals were positive for morbillivirus antigen. However, the serological data are suggestive of a continuously circulating morbillivirus among harbour porpoises from the Black Sea indicating that harbour porpoises may serve as carriers for fatal diseases in susceptible cetacean species.
Asunto(s)
Infecciones por Morbillivirus/veterinaria , Morbillivirus/aislamiento & purificación , Marsopas/virología , Animales , Anticuerpos Antivirales/sangre , Antígenos Virales/análisis , Encéfalo/virología , Bulgaria , Femenino , Georgia (República) , Inmunohistoquímica , Pulmón/virología , Masculino , Infecciones por Morbillivirus/sangre , Infecciones por Morbillivirus/virología , Pruebas de Neutralización/veterinaria , Bazo/virología , UcraniaRESUMEN
The pathological, microbiological and serological findings in harbour porpoises hunted in Greenlandic waters were compared with the findings in animals accidentally caught in fishing gear in the German North Sea and Baltic Sea. The body condition of the Greenlandic animals was good, whereas nine of 23 German harbour porpoises were moderately to markedly emaciated. Both groups were infested with parasites. In the Greenlandic animals parasitism of the aural peribullar cavity with Stenurus minor, of the liver and pancreas with Orthosplanchnus mironovi, of the lungs with Halocercus species and of the subcutaneous and mammary tissue with Crassicauda species was generally associated with a mild inflammatory response. No diseases associated with bacteria were identified in any of the Greenlandic harbour porpoises. In the porpoises from the German North Sea and Baltic Sea, parasites were present in the aural peribullar cavity (S minor), liver (Campula oblonga), first and second gastric compartment (Anisakis simplex) and in the lungs (Pseudalius inflexus and Torynurus convolutus). Moderate to marked pulmonary parasitism and suppurative pneumonia, not observed in the Greenlandic porpoises, were present in 11 and 10, respectively, of the 23 German porpoises. The suppurative pneumonia was attributed to bacterial infection with beta-haemolytic streptococci and Escherichia coil var haemolytica. Four Greenlandic and 10 German porpoises had positive porpoise morbillivirus-specific antibody titres suggesting that the virus was circulating in both populations.
Asunto(s)
Infecciones Bacterianas/veterinaria , Enfermedades Parasitarias en Animales/patología , Marsopas , Animales , Autopsia , Infecciones Bacterianas/epidemiología , Infecciones Bacterianas/patología , Femenino , Groenlandia/epidemiología , Inflamación , Pulmón/microbiología , Pulmón/parasitología , Pulmón/patología , Masculino , Enfermedades Parasitarias en Animales/epidemiología , Marsopas/microbiología , Marsopas/parasitología , Marsopas/virologíaRESUMEN
Causes of canine juvenile hydrocephalus have been well documented. However, the effects of hydrocephalus on periventricular white matter have been only partially described. The present report shows that hydrocephalus-associated lesions of the periventricular white matter, i.e., formation of diverticula, clefts, and tears, are prevalent. Marked hydrocephalus was identified at necropsy in 20 juvenile dogs between 1990 and 1999. The severity grade was based upon a ratio of lateral ventricular dimensions to cortical thickness. All animals exhibited ependymal lesions consisting of attenuation, with or without abortive attempts of ependymal regeneration, and ulceration. In 10 dogs (50%), unilateral or bilateral periventricular diverticula and cleft formation in the region of the caudate nucleus were observed. The diverticula were formed at the caudal pole of the caudate nucleus, communicated with the ventricular lumen, and were associated with ependymal denudation. Loss of the ependymal lining probably contributes to a bulk shift of cerebrospinal fluid from the ventricular lumen to the periventricular white matter, leading to diverticulum formation. Clefts were observed within the parenchyma at the border of the internal capsule and putamen, consistent with an ischemic insult. Occasionally tearing with separation of the caudate nucleus from the subcortical white matter was found, representing unification of expanding clefts and diverticula. In one of the few clinically well-documented cases, tearing was correlated with a sudden decline in neurologic function, culminating in euthanasia. However, tears and clefts may exhibit a chronicity of several days, as indicated by the presence of astroglial scars along the lesion margins.
Asunto(s)
Encéfalo/patología , Enfermedades de los Perros/patología , Hidrocefalia/veterinaria , Animales , Ventrículos Cerebrales/patología , Perros , Femenino , Histocitoquímica , Hidrocefalia/patología , MasculinoRESUMEN
Between 1991 and 1996, necropsies were performed on 445 harbour porpoises (Phocoena phocoena), in various states of preservation, stranded on German coasts or accidentally caught by German fishermen. The animals originated from the North and Baltic Seas, and 133 were considered suitable for histopathological, immunohistochemical and microbiological examination. Most of the lesions in these 133 porpoises were caused by parasites, in particular in the respiratory tract, two-thirds of the animals exhibiting pneumonia associated with the parasites. Pneumonia was considered to be the cause of death in 46% of the stranded subadult and adult animals. The findings gave no evidence of any epidemic due to bacterial or viral infection. Bacteriological examination suggested that pneumonia was mainly caused by secondary bacterial infection and not by parasitic infestation alone. Beta-haemolytic streptococci were considered to be the main infectious agents. Morbillivirus antigen was not detected immunohistochemically.
Asunto(s)
Infecciones Bacterianas/veterinaria , Enfermedades Parasitarias en Animales/patología , Neumonía/veterinaria , Marsopas/parasitología , Virosis/veterinaria , Animales , Autopsia/veterinaria , Infecciones Bacterianas/epidemiología , Infecciones Bacterianas/patología , Causas de Muerte , Femenino , Alemania/epidemiología , Inmunohistoquímica/veterinaria , Masculino , Mar del Norte , Enfermedades Parasitarias en Animales/epidemiología , Neumonía/epidemiología , Neumonía/parasitología , Neumonía/patología , Neumonía/virología , Marsopas/microbiología , Embarazo , Virosis/epidemiología , Virosis/patologíaRESUMEN
Neurologic disease in horses caused by Sarcocystis neurona is difficult to diagnose, treat, or prevent, due to the lack of knowledge about the pathogenesis of the disease. This in turn is confounded by the lack of a reliable equine model of equine protozoal myeloencephalitis (EPM). Epidemiologic studies have implicated stress as a risk factor for this disease, thus, the role of transport stress was evaluated for incorporation into an equine model for EPM. Sporocysts from feral opossums were bioassayed in interferon-gamma gene knockout (KO) mice to determine minimum number of viable S. neurona sporocysts in the inoculum. A minimum of 80,000 viable S. neurona sporocysts were fed to each of the nine horses. A total of 12 S. neurona antibody negative horses were divided into four groups (1-4). Three horses (group 1) were fed sporocysts on the day of arrival at the study site, three horses were fed sporocysts 14 days after acclimatization (group 2), three horses were given sporocysts and dexamethasone 14 days after acclimatization (group 3) and three horses were controls (group 4). All horses fed sporocysts in the study developed antibodies to S. neurona in serum and cerebrospinal fluid (CSF) and developed clinical signs of neurologic disease. The most severe clinical signs were in horses in group 1 subjected to transport stress. The least severe neurologic signs were in horses treated with dexamethasone (group 3). Clinical signs improved in four horses from two treatment groups by the time of euthanasia (group 1, day 44; group 3, day 47). Post-mortem examinations, and tissues that were collected for light microscopy, immunohistochemistry, tissue cultures, and bioassay in KO mice, revealed no direct evidence of S. neurona infection. However, there were lesions compatible with S. neurona infection in horses. The results of this investigation suggest that stress can play a role in the pathogenesis of EPM. There is also evidence to suggest that horses in nature may clear the organism routinely, which may explain the relatively high number of normal horses with CSF antibodies to S. neurona compared to the prevalence of EPM.
Asunto(s)
Modelos Animales de Enfermedad , Encefalomielitis/veterinaria , Enfermedades de los Caballos/parasitología , Sarcocistosis/veterinaria , Estrés Fisiológico/veterinaria , Animales , Western Blotting/veterinaria , Dexametasona/farmacología , Encefalomielitis/complicaciones , Enfermedades de los Caballos/etiología , Caballos , Inmunosupresores/farmacología , Ratones , Ratones Noqueados , Zarigüeyas/parasitología , Factores de Riesgo , Sarcocistosis/etiología , Estrés Fisiológico/complicaciones , TransportesRESUMEN
To facilitate a detailed investigation of cetacean lymphoid organs, 13 canine-, six bovine-, one equine-, one human- and four killer whale-specific monoclonal antibodies (mAbs) directed against cell surface antigens of the haematopoietic system (including CD2, CD4, CD8, CD45R, MHC class II, granulocyte, thrombocyte, pan-T cell and B-cell antigen), as well as a mAb and a polyclonal antibody (pAb) directed against the -peptide of the human CD3 complex, were tested for immunohistochemical cross-reactivity on frozen or formalin-fixed, paraffin wax-embedded lymphatic tissues of harbour porpoises. Eight of 26 mAbs and the pAb showed a specific reaction with harbour porpoise cells. Lymphocytes in T-cell compartments were labelled by the mAb and the pAb directed against the CD3 complex and by two killer whale mAbs specific for CD2 antigen. CD45R, labelled by a killer whale-specific mAb, was strongly expressed on B and weakly on T cells. MHC class II antigen, recognized by killer whale- and bovine-specific mAbs, was expressed on B and T cells. A canine MHC class II-specific mAb recognized an epitope on the surface of antigen-presenting cells and B lymphocytes. An anti-equine-pan-leucocyte marker labelled the majority of cells in B- and T-cell compartments. Thus, with leucocyte antigen markers from various species, it is now possible to determine the phenotype of lymphocytes in normal and diseased lymphoid organs of harbour porpoises.
Asunto(s)
Sistema Linfático/inmunología , Marsopas/inmunología , Animales , Anticuerpos Monoclonales/inmunología , Antígenos de Superficie/inmunología , Bovinos , Reacciones Cruzadas , Perros , Técnica del Anticuerpo Fluorescente Indirecta , Humanos , Técnicas para Inmunoenzimas , Linfocitos/citología , Linfocitos/inmunología , Especificidad de la EspecieRESUMEN
A 10-week-old male Great Dane Puppy was presented for sudden onset tetraataxia and severe paresis of the front legs. Mineral deposits were detected radiographically, at gross postmortem examination, and light microscopically between the vertebral arches of multiple cervical and lumbar vertebrae. These deposits were associated with the interarchial ligaments (ligamentia interarcualia), along the interfaces of the synovium and articular cartilage of multiple cervical, thoracic, and lumbar facets, on the dorsal aspect of several thoracic intervertebral discs, and at the insertion of muscles at the lateral aspect of several cervical and thoracic vertebral bodies. The mineral deposits were associated with a granulomatous inflammation and synovial fibrocartilaginous metaplasia and proliferation, which was focally exuberant. X-ray diffraction analyses of the mineral deposits revealed calcium hydroxylapatite as the major component. The clinical signs in this puppy were due to focal compression of the spinal cord by marked extraarticular ligament-associated fibrocartilaginous proliferation.
Asunto(s)
Densidad Ósea , Calcinosis/veterinaria , Enfermedades de los Perros/patología , Durapatita , Animales , Antiinflamatorios/uso terapéutico , Calcinosis/tratamiento farmacológico , Calcinosis/patología , Cartílago Articular/patología , Enfermedades de los Perros/tratamiento farmacológico , Perros , Masculino , Paresia/veterinaria , Prednisolona/uso terapéutico , Médula Espinal/patología , Membrana Sinovial/patología , Difracción de Rayos XRESUMEN
The role of morbillivirus infection as a cause of disease or death in harbour porpoises (Phocoena phocoena) from the German North and Baltic Sea was investigated by serology, histology and immunohistochemistry. Blood and tissue samples of lung, brain and lymph nodes from 74 stranded or by-caught harbour porpoises from German waters were collected between 1991 and 1997. According to dentinal growth layers and body length, animals were grouped into four age classes (neonates, 0-1, 1-4, 4-16 years of age). Formalin-fixed, paraffin-embedded sections were stained by hematoxylin and eosin (HE). Immunohistology was done in all lung tissues using the avidin-biotin-peroxidase technique and a polyclonal canine distemper virus (CDV) nucleoprotein-specific antibody, which cross-reacts with porpoise morbillivirus (PMV) antigen. A virus neutralization assay for detection of (Onderstepoort-strain) CDV- and PMV-specific antibodies was performed. Due to the cytotoxicity of some sera, only titres of 1:20 or greater were considered positive. PMV or CDV-specific neutralizing antibody titres were found in 88 and 50% of the animals, respectively. Titres were always highest against PMV indicating infection with a homologous porpoise virus strain. There were no significant differences in neutralizing antibody titres between animals of the different age groups. No histological lesions specific for morbillivirus infection were detected and by immunohistology all cases were negative for morbillivirus antigen. The absence of morbillivirus antigen and the lack of characteristic morbillivirus-specific lesions showed that morbillivirus infection was not a cause of death or illness in the investigated population. However, the high incidence of PMV-specific antibodies in all age groups indicated a continuous spread of infection with a morbillivirus among harbour porpoises from the German Baltic and North Sea.
Asunto(s)
Infecciones por Morbillivirus/veterinaria , Morbillivirus/aislamiento & purificación , Marsopas/virología , Factores de Edad , Animales , Anticuerpos Antivirales/sangre , Antígenos Virales/análisis , Encéfalo/virología , Femenino , Alemania/epidemiología , Inmunohistoquímica , Pulmón/virología , Ganglios Linfáticos/virología , Masculino , Morbillivirus/inmunología , Morbillivirus/patogenicidad , Infecciones por Morbillivirus/sangre , Infecciones por Morbillivirus/epidemiología , Infecciones por Morbillivirus/virología , Pruebas de Neutralización/veterinaria , Estudios SeroepidemiológicosRESUMEN
Ovarian cysts and one ovarian tumour, uterine tumours, vaginal calculi, abscesses of the broad ligament or undetermined testicular lesions were observed in 25 out of 502 female and male dusky dolphins (Lagenorhynchus obscurus) caught off Peru in 1985-1987 or 1993-1994. Tentative or definitive diagnoses included Graafian follicle cysts, luteinized cyst, ovarian parasitic granulomatous inflammation, dysgerminoma, leiomyoma, fibroleiomyoma and chronic fibrino-suppurative inflammation of the broad ligament. All lesions described represented first reports for L. obscurus, and the diagnosis of dysgerminoma was the first in a cetacean. It is also the first time that trematode eggs have been reported in the ovaries of cetaceans and that a vaginal calculus has been encountered in a sexually immature cetacean. The finding of struvite as a major component in two vaginal calculi suggested an infectious aetiology. Of 11 mature females with ovarian tumour or cysts or uterine tumours, only one (9.1%) was pregnant, i.esignificantly less than the expected pregnancy rate (53.3% in a random sample of Peruvian dusky dolphins). Several females with ovarian or uterine lesions and males with aberrant testes were large animals. It is possible that some of these lesions were associated with normal senescence of the reproductive system.