RESUMEN

An enterokinase (Enteropeptidase, EC. 3.4.21.9) has been described in the pharate adult of Glossina mositans morsitans. The enzyme is present in pharate adults, 21 days after pupation. It activated commercial crystalline bovine trypsinogen to trypsin. It showed affinity for concanavalin A bound to sepharose and was reversibly sensitive to boiling at pH 6.0. The apparent molecular weight, as determined by gel permeation on sepharose 6B-CL, suggests self-aggregation or an association with a large molecule (M.Wt. approximately equal to 2.5 X 10(6)).

Asunto(s)

Endopeptidasas/aislamiento & purificación , Enteropeptidasa/aislamiento & purificación , Moscas Tse-Tse/enzimología , Animales , Cromatografía de Afinidad , Cromatografía en Gel , Enteropeptidasa/metabolismo , Activación Enzimática , Peso Molecular , Pupa/enzimología , Tripsina/metabolismo , Tripsinógeno/metabolismo , Moscas Tse-Tse/crecimiento & desarrollo