RESUMEN
The efficacy of several antibiotic treatments to eliminate mycoplasma from Vero cells contaminated chronically with Mycoplasma orale II were tested. Minocyclin, Kanamycin, Tylosine and Roxitromycin, at non cytotoxic concentrations, were assayed alone or in different combinations. Mycoplasma contamination was effectively eradicated without recurrence once the following regimen was applied: Incubation of contaminated cells with Tylosine (250 micrograms/ml) for 12 days followed by incubation with Minocycline (5 micrograms/ml) for 10 days. This treatment was not deleterious for cell growth, it was effective after only one application and it was successful to eradicate mycoplasma from other contaminated eukaryotic continuous cell lines.
Asunto(s)
Técnicas de Cultivo/métodos , Quimioterapia Combinada/farmacología , Mycoplasma/efectos de los fármacos , Animales , Células Cultivadas , Chlorocebus aethiops , Cricetinae , Farmacorresistencia Microbiana , Células Eucariotas/microbiología , Humanos , Kanamicina/farmacología , Minociclina/farmacología , Mycoplasma/aislamiento & purificación , Roxitromicina/farmacología , Células Tumorales Cultivadas , Tilosina/farmacologíaRESUMEN
Bacillus subtilis 430A, isolated from the Vernonia herbacea (Vell Rusby) rhizosphere, produced an exocellular inulinase that fits the requirements for the production of syrups on an industrial scale. The partially purified enzyme, obtained by acetone precipitation, displayed a higher specificity for inulin (Km, 8 mM) than for sucrose (56 mM) and a total invertase/total inulase ratio of 0.62. In addition, it is stable at an optimal temperature of 45 to 50 degrees C for at least 7 h and is inhibited by the end product, fructose, at 14 mM.