RESUMEN
Calluna vulgaris L. Hull (Ericaceae) has been used for treatment of urinary tract infections in traditional medicine. In this study we analyzed in vitro antibacterial activity of the plant extracts on different strains of Escherichia coli, Enterococcus faecalis and Proteus vulgaris, as well as the concentrations of total phenols and flavonoids in the extracts. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined. The concentrations of total phenols were examined by using Folin-Ciocalteu reagent and ranged between 67.55 to 142.46 mg GAE/g. The concentrations of flavonoids in extracts were determined using spectrophotometric method with aluminum chloride and the values ranged from 42.11 to 63.68 mg RUE/g. The aqueous extract of C. vulgaris showed a significant antibacterial activity. The values of MIC were in the range from 2.5 mg/ml to 20 mg/ml for this extract. Proteus vulgaris strains were found to be the most sensitive. The results obtained suggest that all tested extracts of C. vulgaris inhibit the growth of human pathogens, especially the aqueous extract.
Asunto(s)
Calluna , Fitoterapia , Extractos Vegetales/farmacología , Infecciones Urinarias/tratamiento farmacológico , Infecciones Urinarias/microbiología , Antibacterianos/química , Antibacterianos/farmacología , Calluna/química , Enterococcus faecalis/efectos de los fármacos , Escherichia coli/efectos de los fármacos , Flavonoides/química , Flavonoides/farmacología , Humanos , Técnicas In Vitro , Pruebas de Sensibilidad Microbiana , Fenoles/química , Fenoles/farmacología , Extractos Vegetales/química , Proteus vulgaris/efectos de los fármacosRESUMEN
Antibacterial and antioxidant activity, total phenolic and flavonoid concentrations of aqueous, ethanol and ethyl acetate extracts from the leaves and flowers of Erica herbacea L. were studied. In vitro antibacterial activity of the extracts was determined by macrodilution method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) have been determined. Testing was performed on 30 clinical isolates, including different strains of Escherichia coli, Enterococcus faecalis and Proteus vulgaris. The values for MIC were in the range from 2.5 mg/mL to 40 mg/mL. The most sensitive bacterial strains were Proteus vulgaris strains. The aqueous extract from E. herbacea was found the most active. The total phenolic content was determined using Folin-Ciocalteu reagent and ranged between 14.98 and 119.88 mg GA/g. The concentration of flavonoids in extracts was determined using spectrophotometric method with aluminium chloride and obtained results varied from 16.19 to 26.90 mg RU/g. Antioxidant activity was monitored spectrophotometrically using DPPH reagent. The highest capacity to neutralize DPPH radicals was found in the aqueous extract from E. herbacea. The results of the total phenolic content determination of the examined extracts indicate that E. herbacea extracts are a rich source of phenolic compounds and also possess a significant antioxidant activity and moderate antibacterial activity.
Asunto(s)
Antibacterianos/farmacología , Antioxidantes/farmacología , Ericaceae , Fenoles/farmacología , Extractos Vegetales/farmacología , Antibacterianos/química , Antibacterianos/aislamiento & purificación , Antioxidantes/química , Antioxidantes/aislamiento & purificación , Compuestos de Bifenilo/química , Relación Dosis-Respuesta a Droga , Enterococcus faecalis/efectos de los fármacos , Enterococcus faecalis/crecimiento & desarrollo , Ericaceae/química , Escherichia coli/efectos de los fármacos , Escherichia coli/crecimiento & desarrollo , Flavonoides/química , Flavonoides/aislamiento & purificación , Flavonoides/farmacología , Flores , Pruebas de Sensibilidad Microbiana , Fenoles/química , Fenoles/aislamiento & purificación , Fitoterapia , Picratos/química , Extractos Vegetales/química , Extractos Vegetales/aislamiento & purificación , Hojas de la Planta , Plantas Medicinales , Proteus vulgaris/efectos de los fármacos , Proteus vulgaris/crecimiento & desarrolloRESUMEN
PURPOSE: Our aim is to report the co-existence of Duane's retraction syndrome and Wyburn-Mason syndrome, a rare condition characterized by arteriovenous malformations (AVMs) in the central nervous system and retina. METHODS: An 11-year-old boy was referred for evaluation of strabismus present since birth. On examination his uncorrected visual acuity was 6/6 in each eye, with small angle left eye esotropia in the primary position, ipsilateral face turn, abduction deficit, lid fissure narrowing in adduction and widening in abduction; plus typical features of left-sided type I Duane syndrome were present. The left fundus demonstrated localized, well-compensated, markedly convoluted, dilated and tortuous retinal vessels and venous congenital retinal macrovessel, which traverse the macular region. The right fundus was unremarkable. In order to elucidate if this retinal vascular malformation is associated with similar lesions in the orbit, paranasal sinuses and/or brain, magnetic resonance imaging (MRI) was performed, followed by digital subtraction angiography of the brain vessels. RESULTS: Clinical features of retinal disease (typical retinal vascular malformation) and brain imaging studies have led to the diagnosis of Wyburn-Mason syndrome. Digital subtraction angiography of the brain revealed an AVM of 30 mm maximal diameter in the left occipital lobe. CONCLUSIONS: Intracranial AVM might have affected the normal vascular development during the second month of gestation, causing involutional changes of the sixth nerve and resulted in Duane's syndrome by "steal phenomenon." Retinal AVM may point to concomitant intracranial AVMs, thus warranting neurological assessment. Patients with AVM of the retina should be examined early with brain and orbital neuroimaging to rule out cerebral AVMs.