RESUMEN

Many studies have comprehensively examined the venom of Ophiophagus hannah snake. Its venom comprises different compounds exhibiting a wide range of pharmacological activities. In this investigation, four peptide fractions (PFs), ranging from 3 kDa to 10 kDa, isolated from the Vietnamese snake venom of O. hannah were separated by HPLC and investigated for their inhibitory activity on adipogenesis in 3T3-L1 adipocytes. The most effective PF was then further purified, generating two peptides, pOh1 and pOh2. Upon investigation of these two peptides on 3T3-L1 adipocytes, it was revealed that, at 10 µg/mL, pOh2 was able to inhibit the lipid accumulation in 3T3-L1 adipocytes by up to 56%, without affecting cell viability. Furthermore, the pOh2 downregulated the gene expression of important transcription factors C/EBP-α and PPAR-γ. In addition, aP2 and GPDH adipocyte-specific markers were also significantly reduced compared to untreated differentiated cells. Taken together, pOh2 inhibited the expression of key transcription factors C/EBP-α and PPAR-γ and their target genes, aP2 and GPDH, thereby blocking the adipocyte differentiation. In conclusion, this novel class of peptide might have potential for in vivo antiobesity effects.

Asunto(s)

Adipocitos/metabolismo , Adipogénesis/efectos de los fármacos , Proteína alfa Potenciadora de Unión a CCAAT/metabolismo , PPAR gamma/metabolismo , Péptidos/farmacología , Células 3T3-L1 , Adipocitos/efectos de los fármacos , Animales , Proteína alfa Potenciadora de Unión a CCAAT/genética , Cromatografía Líquida de Alta Presión , Metabolismo de los Lípidos/efectos de los fármacos , Metabolismo de los Lípidos/genética , Ratones , PPAR gamma/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , Venenos de Serpiente/química