RESUMEN
BACKGROUND: Neuroborreliosis affects peripheral and central nervous system. OBJECTIVES: Point out on possibilities of laboratory diagnostics of neuroborreliosis. SUBJECTS AND METHODS: During 1997-2001 we tested 666 pair samples of CSF and serum from 661 patients with different neurological diagnosis by ELISA, Westernblot, PCR, completed by biochemical and cytological investigations. RESULTS: We confirmed intrathecal specific IgG antibodies production by AI in 14 cases (2.1%) of total 666 samples tested. From those in 7 cases there were present also IgM antibodies in CSF. We found borderline AI values in 3 cases (0.5%) and isolated intrathecal production, antibodies present only in CSF, in 1 case (0.15%). There were normal AI values found in 25 cases (3.8%). Specific antibody possitivity by WB method was detected only in one case. DNA positivity by PCR was detected in one CSF from 43 samples during 2 years period. CONCLUSIONS: The microbiological test results should not be used in isolation but used in correlation with the biochemical and cytologic tests and also with clinical symptoms and epidemiological data to produce an overall clinical diagnosis. (Tab. 7, Fig. 1, Ref. 21)
Asunto(s)
Anticuerpos Antibacterianos/análisis , Grupo Borrelia Burgdorferi/inmunología , Neuroborreliosis de Lyme/diagnóstico , Adolescente , Adulto , Anciano , Niño , Femenino , Humanos , Inmunoglobulina G/análisis , Inmunoglobulina M/análisis , Masculino , Persona de Mediana Edad , Pruebas SerológicasRESUMEN
BACKGROUND: Human cytomegalovirus is widely spread in the human population and at present it is the most important clinical agent of opportunistic infections in immunocompromised patients. Bone marrow transplant recipients, solid organ transplant recipients and AIDS patients are at risk of the development of a severe systemic disease. Prompt detection of active infection is essential for early initiation of antiviral therapy. AIM: The aim of our work was to follow the occurrence of symptomatic CMV infection in heart recipients. METHODS: We have used antigenemia assay, PCR and serology methods for the detection of CMV infection in heart transplant recipients. RESULTS: We found that the occurrence of symptomatic CMV infections was lower than the literary data. CONCLUSION: Though a small group of patients was examined, we suppose that this was caused by the careful finding of CMV serostatus of donors and recipients, by using of CMV-seronegative, filtered, or leukocytes-poor blood products, by the examination of blood products and prophylaxis of risk patients, too. (Tab. 1, Ref. 37.).