RESUMEN
Results of epidemiologic, virologic, and serologic studies of enterovirus infections in Khabarovsk region from 1975 to 2006 were analyzed. Patterns of epidemic process of these infections were established: periodic change of dominating type of pathogen in the population; onset of the large epidemic peaks of incidence during emergence of circulation of new for the given area serotypes of enteroviruses; possibility of realization of several routes of virus transmission. Role of water factor in the progress of the epidemic process was revealed. Etiology of the large epidemic rise of aseptic meningitis incidence in Khabarovsk region in 2006 was established--the leading pathogens were ECHO viruses serotypes E6 and E30.
Asunto(s)
Brotes de Enfermedades , Infecciones por Enterovirus/epidemiología , Meningitis Aséptica/epidemiología , Anticuerpos Antivirales/sangre , Notificación de Enfermedades , Enterovirus/clasificación , Enterovirus/genética , Enterovirus/inmunología , Enterovirus/aislamiento & purificación , Infecciones por Enterovirus/sangre , Infecciones por Enterovirus/virología , Humanos , Incidencia , Meningitis Aséptica/sangre , Meningitis Aséptica/virología , Pruebas de Neutralización , Estudios Retrospectivos , Factores de Riesgo , Estaciones del Año , Siberia/epidemiología , Microbiología del AguaRESUMEN
Using a commercial diagnostic preparation for indirect IFA test, 135 urine samples from 50 patients with HFRS were examined at different periods of the disease. Antibodies were demonstrable in all urine specimens from HFRS patients for 13 days. In 14-20 days they could be detected in half of the patients, and no antibodies could be demonstrated since the 21st day on. The results of urine examination from healthy subjects and some patients with other clinical diagnoses were negative same as controls with normal antigen. The dynamics of antibody titres in the patients' urine differed from that in the blood and was considered as "decreasing" similarly as the clinical disease. The antibody excretion in the urine coincided with the period of renal structure damage and stopped when the normal renal function was restored. The data are discussed from the point of view of the pathogenesis and diagnosis. Special attention was paid to the possibility and advantages of early HFRS diagnosis by antibody determinations in the patients' urine.
Asunto(s)
Anticuerpos Antivirales/orina , Virus Hantaan/inmunología , Fiebre Hemorrágica con Síndrome Renal/diagnóstico , Animales , Técnica del Anticuerpo Fluorescente , Fiebre Hemorrágica con Síndrome Renal/orina , Humanos , Masculino , Factores de Tiempo , Células Vero , Cultivo de VirusRESUMEN
Consecutive serum samples collected from 235 patients with Haemorrhagic Fever with Renal Syndrome (HFRS), between two days and two years after onset of disease, have been analysed for the presence of IgG and IgM type of antibodies specific for Hanta-viruses. The sera were screened in parallel by a newly developed indirect Immuno Enzyme Assay (EIA) in parallel with Indirect Immunofluorescent Antibody Assay (IFA). In both tests the Hantaan virus strain 76-118 was used as the antigen. The EIA was much more sensitive than the IFA test for the detection of IgM type antibodies. With the indirect EIA IgM type antibodies against Hantaan virus 76-118 have been detected in HFRS patient's sera from the second day of illness indicating the usefulness of this test for the early serological diagnosis of this disease.
Asunto(s)
Anticuerpos Antivirales/análisis , Fiebre Hemorrágica con Síndrome Renal/inmunología , Técnica del Anticuerpo Fluorescente , Humanos , Técnicas para Inmunoenzimas , Inmunoglobulina G/análisis , Inmunoglobulina M/análisis , Factores de TiempoAsunto(s)
Anticuerpos Antivirales/biosíntesis , Fiebre Hemorrágica con Síndrome Renal/inmunología , Orthohantavirus/inmunología , Anticuerpos Antivirales/análisis , Especificidad de Anticuerpos , Antígenos Heterófilos/inmunología , Antígenos Virales/inmunología , Humanos , Siberia , Factores de TiempoRESUMEN
In natural foci of hemorrhagic fever with renal syndrome (HFRS) located in agricultural landscapes, rodents of various species are infected with the virus, however, regular HFRS epizootics are observed in field mice which present the highest abundance of infected animals in the population. Annually, a high direct correlation is observed between the population density of rodents and the percentage of animals with HFRS virus antigen in the blood and lungs. In 1981, there was a high correlation between the occurrence of rodents with Gamasid mites and the presence of the antigen in the blood. All the foregoing suggests that HFRS agent exists in natural foci due to a combination of different transmission mechanisms (obligate-transmissive mechanism) and penetrates into murine rodents by various modes: air--dust-borne and/or alimentary and transmissive. Non-transmissive modes are likely to be the leading ones.