RESUMEN
The treatment of streptococci, groups C and G, with bromocyanogen made it possible to isolate surface G protein, capable of binding human serum albumin (HSA) and polyclonal IgG. In this work the presence of G protein in all staphylococcal strains, groups C and G, is shown. The differences between the strains by the level of expression, molecular weight and functional activity of G protein, extracted from streptococci of groups C and G, permitted the identification of 3 groups of strains, containing the molecules of G protein with different numbers of IgG- and HSA-binding domains: with 3 IgG- and HSA-binding domains, with 2 IgG- and HSA-binding domains and with only 2 IgG-binding domains. Each strain under study expressed only one of the molecule of G protein. The work shows the possibility of the identification of streptococci, groups C and G, by the molecular characteristics of G proteins themselves and their respective coding genes.
Asunto(s)
Proteínas Bacterianas/aislamiento & purificación , Streptococcus/clasificación , Proteínas Bacterianas/genética , Proteínas Bacterianas/metabolismo , Bromuro de Cianógeno , Humanos , Peso Molecular , Reacción en Cadena de la Polimerasa , Unión Proteica , Albúmina Sérica/metabolismo , Streptococcus/genética , Streptococcus/aislamiento & purificaciónRESUMEN
Methods of ELISA competitive binding and blotting on nitrocellulose membranes were developed for detecting microalbuminuria in diabetic nephropathy. These methods are based on the use of recombinant albumin receptor. They are highly specific and sensitive and are recommended for everyday clinical use.