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[Cloning chloroplast DNA in escherichia coli. I. Construction and selection of recombinant plasmids containing fragments of pea chloroplast DNA]. / Klonirovanie DNK khloroplastov v Escherichia coli. Soobshchenie I. Konstruktsiia i selektsiia rekombinantnykh plazmid, soderzhashchikh fragmenty DNK khloroplastov gorokha.

Andrianov, V M; Zemlianaia, N Iu; Karimov, M; Amerkhanova, M B; Vinetskii, Iu Ts.

Genetika ; 14(9): 1503-12, 1978 Sep.

Artículo en Ruso | MEDLINE | ID: mdl-363514

RESUMEN

Fragments produced by digestion of Pisum sativum chloroplast DNA with EcoRI were examined by agarose gel electrophoresis. These EcoRI-fragments were joined in vitro to Apr-ColE1 RSF2124 plasmid and cloned in Escherichia coli. Methods of molecular cloning of plasmid chimeras by success gradient centrifugation and repeated transformation and selection of recombinant plasmids using mytomicin C were used for cloning hybrid plasmids with various EcoRI fragments of pea chloroplast DNA has been obtained.

Asunto(s)

Cloroplastos/análisis , Replicación del ADN , Escherichia coli/genética , Plásmidos , Recombinación Genética , Células Clonales , Escherichia coli/metabolismo , Hibridación de Ácido Nucleico , Plantas/genética , Transfección

RESUMEN

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