RESUMEN
Rickettsial infections in dogs of Mexico were investigated. A total of 246 dogs were blood sampled and initially screened to detect Ehrlichia canis, E. chaffeensis, E. ewingii, Anaplasma phagocytophilum and Rickettsia rickettsii by a quantitative real-time PCR (qPCR) assay. Sixty-five dogs were monitored and sampled twice 7-8 months apart. Using the qPCR, 72 positive dogs to E. canis were detected (prevalence of 29.26%). These dogs were also tested by nested PCR to detect the same pathogens. None of the studied dogs were positive to E. chaffeensis, E. ewingii, R. rickettsii nor A. phagocytophilum by both PCR assays. The cumulative incidence of E. canis infection was 38.46%. Sequencing analysis of the nested PCR products revealed 100% and 98.1% identity of E. canis and R. parkeri, respectively. We found a dog co-infected with E. canis and R. parkeri.
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Ehrlichia canis/genética , Ehrlichiosis/epidemiología , Ehrlichiosis/veterinaria , Animales , Secuencia de Bases , Coinfección , ADN Bacteriano/genética , Perros , Ehrlichia canis/aislamiento & purificación , Incidencia , México/epidemiología , Reacción en Cadena de la Polimerasa , Prevalencia , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Encuestas y Cuestionarios , Enfermedades por Picaduras de Garrapatas/epidemiología , Enfermedades por Picaduras de Garrapatas/microbiología , Enfermedades por Picaduras de Garrapatas/veterinariaRESUMEN
Twenty-five white-tailed deer (Odocoileus virginianus yucatanensis) (WTD), 4 mazama deer (Mazama temama) (MD), and the ticks they host in Yucatan, Mexico were sampled to run a molecular survey for tick-borne rickettsial agents. The prevalence of rickettsial agents was 20% in WTD (5/25) and 50% in MD (2/4). When sequencing the nested PCR products, E. chaffeensis, A. phagocytophilum and A. odocoilei, were identified as single infection or coinfecting cervids. None of the cervid samples were positive for E. ewingii, E. canis, nor Rickettsia spp. Overall, 355 individual ticks were collected. Species identified based on adult stages infesting cervids included Amblyomma mixtum, A. parvum, A. cf. oblongoguttatum, Ixodes affinis, Rhipicephalus microplus, R. sanguineus sensu lato, and Haemaphysalis juxtakochi. Rhipicephalus microplus was the tick species most commonly found infesting cervids with a frequency of 28.4%, and intensity of 25.2 ticks per animal. A pool of Amblyomma cf. oblongoguttatum adults and one of Amblyomma spp. nymphs were positive for E. canis and E. chaffeensis, respectively. None of the studied tick pools were positive for E. ewingii, A. phagocytophilum, nor R. rickettsii. To the best of our knowledge, this study is the first to report the prevalence of rickettsial agents in WTD and MD in Mexico. Our molecular study is the first to report the detection of E. chaffeensis, A. phagocytophilum, and A. odocoilei in MD in Mexico. The molecular detection of E. chaffeensis, A. phagocytophilum, and A. odocoilei in deer, and E. chaffeensis in Amblyomma spp. nymphs reported here raises the concern for the risk of human exposure to tick-borne rickettsial pathogens. Our findings highlight the need to apply the "One Health" approach to study ticks and tick-borne diseases. This science-based information could be used by state public-health programs to assess the risk for exposure to tick-borne Anaplasmataceae in Yucatan, Mexico.
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Ciervos/microbiología , Ehrlichia chaffeensis/genética , Enfermedades por Picaduras de Garrapatas/veterinaria , Garrapatas/microbiología , Anaplasma phagocytophilum/genética , Anaplasma phagocytophilum/aislamiento & purificación , Animales , Animales Salvajes/microbiología , Animales Salvajes/parasitología , Vectores Arácnidos/microbiología , Coinfección/epidemiología , Coinfección/microbiología , Ehrlichia chaffeensis/aislamiento & purificación , Ehrlichiosis/epidemiología , Ixodes/microbiología , México/epidemiología , Ninfa/microbiología , Reacción en Cadena de la Polimerasa , Prevalencia , Rickettsia/genética , Rickettsia/aislamiento & purificación , Infestaciones por Garrapatas/epidemiología , Infestaciones por Garrapatas/microbiología , Infestaciones por Garrapatas/veterinaria , Enfermedades por Picaduras de Garrapatas/epidemiología , Enfermedades por Picaduras de Garrapatas/microbiologíaRESUMEN
Rickettsial infection in dog-associated ticks in three rural communities of Yucatan, Mexico was investigated using qPCR and nested PCR assays. A total of 319 dogs were studied and ticks samples were collected. A total of 170 dogs were infested with ticks (frequency of 53.4%). Overall, 1,380 ticks representing seven species were collected: Amblyomma mixtum, A. ovale, A. parvum, A. cf. oblongoguttatum, Ixodes affinis, Rhipicephalus microplus, and R. sanguineus sensu lato. The most abundant species was R. sanguineus s.l. with a mean intensity of 7.4 ticks/host. Dogs in the communities of Chan San Antonio and Yaxcheku were 2.84 and 2.41 times more likely to be infected with R. sanguineus compared with Sucopo (p < 0.05). Adult pools of A. mixtum, A. parvum, I. affinis, R. microplus, and A. c.f. oblongoguttatum were negative to E. chaffeensis, E. ewingii, A. phagocytophilum, and R. rickettsii. However, pools of R. sanguineus s.l. adults and A. ovale adults, as well as nymphs of Amblyomma spp. were positive to E. canis. Sequencing analysis of the nested PCR products amplifying the 16S rRNA gene fragment of E. canis confirmed the results and revealed 100% identity with sequences of E. canis. This is the first report worldwide of E. canis infection in A. ovale by PCR. This finding does not necessarily indicate that A. ovale is a competent vector of E. canis because pathogen transmission of this specific tick to a naïve dog remains to be documented. This study documented that different tick species parasitize dogs in Yucatan, Mexico, where R. sanguineus s.l., A. ovale, and nymphs of Amblyomma spp. were shown to be infected with E. canis. These findings highlight the need for control strategies against tick infestations in dogs to prevent the risk of tick-borne disease transmission among companion animal and probably human populations.
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Anaplasmosis/epidemiología , Enfermedades de los Perros/epidemiología , Ehrlichiosis/veterinaria , Ixodidae/microbiología , Ixodidae/fisiología , Infecciones por Rickettsia/veterinaria , Infestaciones por Garrapatas/veterinaria , Anaplasma/aislamiento & purificación , Anaplasmosis/microbiología , Animales , Enfermedades de los Perros/parasitología , Perros , Ehrlichia/aislamiento & purificación , Ehrlichiosis/epidemiología , Ehrlichiosis/microbiología , Larva/microbiología , Larva/fisiología , México/epidemiología , Ninfa/microbiología , Ninfa/fisiología , Filogenia , Reacción en Cadena de la Polimerasa/veterinaria , Prevalencia , ARN Bacteriano/análisis , ARN Ribosómico 16S/análisis , Rickettsia/aislamiento & purificación , Infecciones por Rickettsia/epidemiología , Infecciones por Rickettsia/microbiología , Infestaciones por Garrapatas/epidemiología , Infestaciones por Garrapatas/parasitologíaRESUMEN
Resumen Actualmente, el estudio de la borreliosis canina adquiere mayor relevancia, ya que el perro es considerado como un importante centinela del complejo Borrelia burgdorferi sensu lato, el cual podría desempeñar un papel clave en la dispersión de garrapatas de las áreas selváticas al ambiente doméstico. En México, la distribución y presencia de genoespecies patógenas de B. burgdorferi en perros y sus garrapatas aún no ha sido investigada. Por tal motivo, la presente investigación tiene como objetivo detectar y estimar la prevalencia de B burgdorferi s.l. en perros y sus garrapatas en dos comunidades rurales de Yucatán, México. En cada comunidad se visitaron 50 viviendas donde se estudiaron 144 muestras de sangre de perros por punción de la vena safena, así como la colecta de sus garrapatas. Se colectaron un total de 846 garrapatas de las especies Ixodes affinis (33 / 846), Rhipicephalus sanguineus sensu lato (786 / 846) y Amblyomma mixtum (27 / 846). Para detectar la presencia de B. burgdorferi s.l. en dichas muestras, se amplificó el gen conservado flaB y las lipoproteínas de membrana externa, ospC y p66, mediante el uso de la reacción en cadena de la polimerasa. La prevalencia obtenida en sangre de perros fue de 17.3 % (25 / 144) para flaB, 12.50 % (18 / 144) para el gen p66 y 1.38 % (2 / 144) para el gen ospC. De las garrapatas analizadas, R. sanguineus s.l. tuvo una prevalencia de infección de 0.89 %, A. mixtum de 5.88 % e I. affinis de 15.15 %, siendo esta última especie la que presentó mayor prevalencia. Dos perros y sus garrapatas I. affinis fueron positivos al gen flaB. Solamente una garrapata R. sanguineus s.l. fue positiva al gen p66 y ninguna especie de garrapata fue positiva al gen ospC. Este estudio confirma la existencia de B. burgdorferi s.l. en perros y sus garrapatas en comunidades rurales de Yucatán, México. La detección de Borrelia en perros podría ser un criterio importante para la evaluación del riesgo de borreliosis en humanos, ya que el perro puede emplearse como indicador epidemiológico para la identificación de nuevos focos de esta enfermedad.
Abstract In Mexico, the distribution and the presence of pathogenic genospecies of B. burgdorferi in dogs and their ticks has not been extensively investigated. The study of canine borreliosis is acquiring greater relevance, since the dog is considered to be an important sentinel for pathogens pertaining to the complex Borrelia burgdorferi sensu lato; in addition, dogs could be playing a key role in the spread of ticks from forested areas into the domestic environment. This study aimed to detect and estimate the prevalence of B. burgdorferi s.l. in dogs and their ticks in two rural communities of Yucatán, Mexico. In each community, 50 houses were visited, where 144 blood samples from dogs were studied by puncture of the saphenous vein, as well as the collection of their ticks. To detect the presence of B. burgdorferi s.l. in these samples, the conserved gene flaB, p66 and ospC were PCR amplified. A total of 144 dog blood samples, and 846 of ticks were obtained from the examined animals. Considering tick species, Rhipicephalus sanguineus sensu lato (786 / 846) was common, while Ixodes affinis (33 / 846), and Amblyomma mixtum (27 / 846) resulted less frequent. As per gene conservation, the prevalence of B. burgdorferi in canine blood was 17.3 % (25 / 144) to flaB, 12.50 % (18 / 144) for p66 and 1.38 % (2 / 144) for the ospC gene. Within the analyzed ticks, R. sanguineus s.l. had a prevalence of 0.89 %, A. mixtum 5.88 % and I. affinis 15.15 %, being this last species the one that presented higher prevalence. Two dogs and their ticks I. affinis were positive to the flaB gene. Only a tick R. sanguineus s.l. was positive to the gene p66 and no tick species was positive the ospC gene. This study confirmed the existence of B. burgdorferi s.l. in dogs and their ticks in rural communities of Yucatán, Mexico. The detection of Borrelia in dogs may be an important criterion for the evaluation of the risk of borreliosis in humans, since the dog can be used as an epidemiological indicator for the identification of new outbreaks of this disease. Rev. Biol. Trop. 66(1): 428-437. Epub 2018 March 01.
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Introducción. La enfermedad de Lyme es una zoonosis multisistémica causada por Borrelia burgdorferi sensu lato. Esta espiroqueta circula en un ciclo enzoótico entre un reservorio vertebrado primario y las garrapatas. Se ha encontrado que varias especies de roedores son eficientes reservorios naturales de B. burgdorferi s.l. Objetivo. Estimar la prevalencia de B. burgdorferi s.l. en roedores sinantrópicos en dos comunidades rurales de Yucatán, México. Materiales y métodos. Se capturaron 123 roedores (94 Mus musculus y 29 Rattus rattus ) para obtener muestras de tejidos de oreja y vejiga. Para la detección de B. burgdorferi s.l. en las muestras, se amplificaron los genes de la flagelina B ( fla B ) y las lipoproteínas de membrana externa, ospC y p66 , mediante reacción en cadena de la polimerasa, y se secuenciaron los amplicones obtenidos. Resultados. La frecuencia de infección por B. burgdorferi s.l. en roedores fue de 36,5 % para flaB (45/123), de 10,5 % (13/123) para p66 y de 3,2 % (4/123) para ospC . En R. rattus la frecuencia de infección fue de 17,2 % y en M. musculus fue de 42,5 %. La frecuencia de infección de B. burgdorferi s.l. en los tejidos estudiados fue de 11,3 % (14/123) en muestras de tejido de vejiga y de 17,0 % (21/123) en las de oreja. No se encontraron diferencias estadísticas (p>0,05) en la frecuencia de infección entre los dos tipos de muestras de tejido utilizadas para el diagnóstico. El gen ospC presentó 98 % de homología con la especie Borrelia garinii , una de las especies heterogéneas del complejo B. burgdorferi s.l. Conclusiones. Los roedores presentaron una alta prevalencia de infección con B. burgdorferi s.l.; las especies M. musculus y R. rattus podrían jugar un papel importante en la continuidad de la presencia de esta bacteria en comunidades rurales de Yucatán, México.
Introduction: Lyme disease is a multisystemic zoonotic disease caused by Borrelia burgdorferi sensu lato. This spirochete circulates in an enzootic cycle between the primary vertebrate reservoir and its tick vectors. Different species of rodents are known to be efficient natural reservoirs for B. burgdorferi s.l. Objective: To estimate the prevalence of B. burgdorferi s.l. in synanthropic rodents from two rural communities of Yucatán, México. Materials and methods: A total of 123 rodents (94 Mus musculus and 29 Rattus rattus ) were trapped, and ear and bladder samples were collected. Flagelin B ( flaB ) genes and outer membrane lipoproteins ospC y p66 were amplified in order to detect B. burgdorferi s.l. presence in the samples. The obtained amplicons were sequenced. Results: The overall infection rates in rodents were 36.5% for flaB (45/123), 10.5% (13/123) for p66, and 3.2% (4/123) for ospC . Rattus rattus had 17.2% of infection and M. musculus , 42.5%. From all examined tissue, 11.3% (14/123) of bladders, and 17.0% (21/123) of ears were infected with the spirochete Borrelia burgdorferi s.l. No statistical differences (p>0.05) were found between the two tissue samples used for diagnosis. The ospC gen was 98% homologous to Borrelia garinii , one species of the B. burgdorferi s.l. complex. Conclusions: We concluded that rodents have a high prevalence of B. burgdorferi s.l. infection, and both species of rodents, M. musculus and R. rattus, might be playing an important role in the maintenance of this bacterium in rural communities of Yucatán, México.
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Borrelia burgdorferi , Enfermedad de Lyme , México , Roedores , Población RuralRESUMEN
INTRODUCTION: Lyme disease is a multisystemic zoonotic disease caused by Borrelia burgdorferi sensu lato. This spirochete circulates in an enzootic cycle between the primary vertebrate reservoir and its tick vectors. Different species of rodents are known to be efficient natural reservoirs for B. burgdorferi s.l. OBJECTIVE: To estimate the prevalence of B. burgdorferi s.l. in synanthropic rodents from two rural communities of Yucatán, México. MATERIALS AND METHODS: A total of 123 rodents (94 Mus musculus and 29 Rattus rattus) were trapped, and ear and bladder samples were collected. Flagelin B (flaB) genes and outer membrane lipoproteins ospC y p66 were amplified in order to detect B. burgdorferi s.l. presence in the samples. The obtained amplicons were sequenced. RESULTS: The overall infection rates in rodents were 36.5% for flaB (45/123), 10.5% (13/123) for p66, and 3.2% (4/123) for ospC. Rattus rattus had 17.2% of infection and M. musculus, 42.5%. From all examined tissue, 11.3% (14/123) of bladders, and 17.0% (21/123) of ears were infected with the spirochete Borrelia burgdorferi s.l. No statistical differences (p>0.05) were found between the two tissue samples used for diagnosis. The ospC gen was 98% homologous to Borrelia garinii, one species of the B. burgdorferi s.l. complex. CONCLUSIONS: We concluded that rodents have a high prevalence of B. burgdorferi s.l. infection, and both species of rodents, M. musculus and R. rattus, might be playing an important role in the maintenance of this bacterium in rural communities of Yucatán, México.
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Proteínas Bacterianas/química , Borrelia burgdorferi/química , Borrelia burgdorferi/aislamiento & purificación , Enfermedad de Lyme/microbiología , Roedores/microbiología , Garrapatas/microbiología , Zoonosis/microbiología , Animales , Proteínas Bacterianas/metabolismo , Secuencia de Bases , Grupo Borrelia Burgdorferi , Enfermedad de Lyme/epidemiología , Enfermedad de Lyme/metabolismo , México , Ratones , Prevalencia , Ratas , Población Rural , Garrapatas/química , Zoonosis/epidemiologíaRESUMEN
The objective of this study was to determine the seroprevalence to Ehrlichia spp. in dogs from Xcalak, Quintana Roo, Mexico, and the associated factors. Serum samples were obtained from 118 dogs and used in an indirect immunofluorescent assay test for the detection of antibodies against Ehrlichia spp. A questionnaire was used to obtain information about possible variables associated with seroprevalence. These variables were analyzed through Chi2 test and logistic regression. Dog seroprevalence of antibodies against Ehrlichia spp. was 64% (75/118). Fifty-two percent (61/118) of dogs had tick infestation which was identified as Rhipicephalus sanguineus sensu lato. Anemia was observed in 36% of dogs. Leucopenia (2.5%), thrombocytopenia (70%), and hemorrhage (14%) were also observed. Thirty-one percent (23/75) of dogs with anemia, 4% (3/75) of dogs with leucopenia, 80% (60/75) of dogs with thrombocytopenia, 17% (13/75) of dogs with hemorrhages, and 59% (44/75) of dogs with ticks were positive for Ehrlichia spp. antibodies. The factors associated with seroprevalence were age (1-3 and >3 years old, OR = 7.77 and OR = 15.39, resp.), tick infestation (OR = 3.13), and thrombocytopenia (OR = 3.36). In conclusion, seroprevalence of Ehrlichia spp. was high in the community of Xcalak and its associated factors were age, tick infestation, and thrombocytopenia.
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Ehrlichia canis is the etiological agent behind canine monocytic ehrlichiosis, and the tick Rhipicephalus sanguineus (Latreille) is its main vector. Blood smear and nested polymerase chain reaction (PCR) techniques were used to identify E. canis infection in dogs and R. sanguineus, and explore factors possibly associated with infection in dogs in Yucatan, Mexico. Blood samples were taken and ticks R. sanguineus collected from 50 dogs (10 house dogs and 40 in an animal control center). Data were collected on dog age, sex, body condition, and signs associated with platelet deficiencies (epistaxis). Blood smears were analyzed to identify E. canis morulae and generate platelet counts. Nested PCR analysis was done on blood samples and 200 ticks. A χ(2) test was done to identify factors associated with the E. canis infection in the tested dogs. The overall prevalence for infection, as determined by PCR, was 36% (18 out of 50). All positive dogs were from samples collected from the animal shelter, representing prevalence, for this sampling site, of 45% (18 out of 40). Morulae in monocytes were identified in only 4% of samples. Dog origin (i.e. animal control center) was the only variable associated with E. canis infection (P < 0.01). Male ticks had a higher (P < 0.05) infection rate than female ticks (24.5 vs 13.5%). It is concluded that E. canis infection is present in both dogs and the brown dog ticks R. sanguineus in Yucatan, Mexico.
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Vectores Arácnidos/microbiología , Enfermedades de los Perros/diagnóstico , Ehrlichia canis/aislamiento & purificación , Ehrlichiosis/veterinaria , Rhipicephalus sanguineus/microbiología , Animales , Enfermedades de los Perros/epidemiología , Enfermedades de los Perros/microbiología , Perros , Ehrlichiosis/diagnóstico , Ehrlichiosis/epidemiología , Ehrlichiosis/microbiología , Femenino , Masculino , México/epidemiología , Reacción en Cadena de la Polimerasa/veterinariaRESUMEN
Los objetivos de este estudio fueron estimar la prevalencia y determinar algunos factores de riesgo asociados con el virus del Síndrome Reproductivo y Respiratorio Porcino (PRRS) en sementales de granjas porcinas de Yucatán, México. El estudio se realizó en 30 granjas con 28 a 2.000 vientres y con diferentes niveles de tecnificación. Los verracos al llegar a la granja fueron sometidos a un periodo de adaptación al manejo y al estatus sanitario. En las granjas se empleaba la monta natural, la inseminación artificial o ambas técnicas. La alimentación de los verracos consistió en alimento comercial. Se realizó un estudio transversal por conglomerados desde septiembre 2005 hasta febrero 2006, muestreándose 170 verracos. La presencia de anticuerpos y partículas virales se determinó mediante las pruebas de ELISA y RT-PCR (Reacción en Cadena de la Polimerasa- Transcriptasa Reversa), respectivamente. Cincuenta y seis verracos fueron positivos a la prueba de ELISA, 21 a la prueba de RT-PCR y 67 a una o ambas pruebas. La prevalencia en los verracos fue 39,4% (67/170) y dentro de granjas varió de 0 a 100%. Veintiún granjas tuvieron al menos un animal positivo a la prueba de ELISA, 13 a la prueba de RT-PCR y 25 a una o ambas pruebas. El riesgo de un animal positivo a PRRS fue 3,6 veces mayor en granjas positivas al virus de PRRS y 2,6 veces mayor en granjas donde no se realizaban pruebas diagnósticas antes de introducir los sementales. Las granjas que utilizaban sementales en la detección de celo tuvieron 7,0 veces mayor riesgo de un verraco positivo al virus de PRRS.
The objectives of this study were to estimate the prevalence of and to determine some risk factors associated with the Porcine Reproductive and Respiratory Syndrome (PRRS) virus in boars from pig farms in Yucatan, Mexico. The study was carried out in 30 farms with 28 to 2,000 sows and different levels of technology. On arrival, boars were kept for an adaptation period to the farm conditions and management. Natural mating, artificial insemination or both techniques were used. Commercial feed was provided to the boars. A cluster cross-sectional study was carried out from September 2005 to February 2006, and 170 boars were sampled. The presence of antibodies and virus particles was determined using ELISA and RT-PCR (Reverse transcriptase- polymerase chain reaction) tests respectively. Fifty six out of 170 boars were positive to ELISA test, 21 to RT-PCR and 67 to one or both tests. Boar prevalence was 39.4% (67/170) and within farm varied from 0 to 100%. Twenty one farms (70%) had at least one ELISA positive animal, 13 positives to RT-PCR test (43.3%) and 25 (83.3%) to one or both tests. The risk of a PRRS virus positive boar was 3.6 times greater in the positive farms and 2.6 when no diagnostic tests were carried out before introducing the boar to the farm. Farms using the boars for estrus detection had 7.0 times greater risk of a PRRS virus seropositive boar.
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Two epidemiological studies were conducted from August 1997 to May 1998: a case-control study to identify herd level risk factors for antibodies to Aujeszky's disease virus (ADV) in sows in the state of Yucatan, Mexico and a cross-sectional study to determine the prevalence of antibodies against ADV in fattening pigs. In the case-control study, data on herd management and biosecurity were obtained from all the 27 ADV known field-virus-seropositive farms (cases) and 62 randomly selected seronegative farms (controls) by questionnaire. Breeding animals of these seropositive farms had received a gE-deletion vaccine. In the cross-sectional study, 26 farrow-to-finish farms of the 27 seropositive farms were used and blood samples taken from 60 fattening pigs per herd (15 pigs for each stage of production). Serum samples were analyzed by the screening-ELISA and gE-ELISA tests. In the case-control study, three of the 15 risk factors were significant. Odds ratios for distance to the nearest farm (< or = 2.5km), not sampling for the detection of ADV and herds with origin of breeding animals within the state were 9.5, 18.1 and 8.7. In the cross-sectional study, 11 (42.3%) of the 26 sampled farms were seropositive to vaccine antibodies. None of the piglets were positive to antibodies against field virus risk--suggesting that the strategy of vaccinating only the breeding animals reduced the ADV infection of the piglets.
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Herpesvirus Suido 1/inmunología , Seudorrabia/etiología , Crianza de Animales Domésticos , Animales , Anticuerpos Antivirales/análisis , Estudios de Casos y Controles , Estudios Transversales , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Herpesvirus Suido 1/patogenicidad , Masculino , México , Oportunidad Relativa , Seudorrabia/diagnóstico , Seudorrabia/inmunología , Factores de Riesgo , Pruebas Serológicas , Porcinos , Vacunación/veterinariaRESUMEN
Introducción. La estrosis es una miasis cavitaria que afecta a los ovinos y caprinos. En la actualidad el diagnóstico es a través de la observación de los signos clínicos y el hallazgo de larvas en los conductos nasales de los animales a la necropsia. Diferentes técnicas serológicas se han utilizado y evaluado para tratar de realizar un mejor diagnóstico de esta enfermedad. Objetivo. Evaluar la prueba de inmunoensayo en capa delgada (ICD) utilizando microplacas de poliestireno, para el diagnóstico de estrosis ovina. Material y métodos. Se utilizaron 95 ovinos sacrificados para abasto, de los cuales se obtuvo el suero y se les realizó el examen postmorten para la detección de la presencia de larvas en los conductos nasales. La presencia de larvas en los conductos nasales fue considerada como prueba de oro. El antígeno usado fue a partir de larvas L2 con una concentración de proteína de 4.2 mg/mL. La prueba se realizó en microplacas de poliestireno de fondo plano y de 96 pozos, se siguió el mismo procedimiento utilizado en la técnica descrita por Bautista 1982. Se determinó la sensibilidad y se midió la concordancia entre ambas pruebas, calculando el valor de kappa. Resultados. De los 95 ovinos sacrificados, 51 (54 por ceinto) fueron positivos, a la preencia de larvas en el examen postmortem y 61 (51 por ciento) a la prueba de ICD y u valor de kappa de 0.53 al comparar ambas pruebas. Conclusiones. El uso de microplacas en la prueba ICD es una alternativa para el diagnóstico de estrosis ovina en estudio epidemiológicos ya que permite con una sensibilidad alta, analizar una mayor cantidad de sueros por placa, utilizando una menor cantidad de antígeno