RESUMEN
Resumen La corteza pre-frontal es la base de las habilidades mentales de mayor complejidad del desarrollo humano. En su proceso de evaluación, la escala EFECO brinda un importante aporte para valorar su estado. En investigaciones previas se ha estudiado esta escala con su configuración de 67 ítems, narrativa centrada en el déficit y evaluación de 8 funciones ejecutiva. En esta investigación se presentan los siguientes aportes: una nueva versión de la escala, centrada en su narrativa en la habilidad ejecutiva, propuesta de ítems para valorar la función ejecutiva de verificación y una versión resumida de la escala en 42 ítems. En el estudio participaron 118 adultos saludables entre 18 y 25 años de edad (M edad = 20.72, DE = 1.65). En los resultados se encontró que la escala EFECO II-VC (versión modificada y completa) obtuvo como consistencia interna α = .96 y sus sub-escalas consistencia interna entre α = .64 y .81. La escala EFECO II-VR (versión modificada y resumida) obtuvo α = .94 y sus sub-escalas entre α = .68 y .79. La consistencia interna de los factores en los que se engloban las funciones ejecutivas se presentaron adecuados: el sistema supervisor de la cognición II-VC α = .93 y II-VR α = .70, mientras que el sistema supervisor de la conducta II-VC α = .93 y II-VR α = .81. Las correlaciones entre las funciones ejecutivas valoradas con ambas escalas fueron entre medianas y grandes r = .36 y .94. Se cierra el trabajo discutiendo el aporte clínico y científico de la modificación de la escala EFECO.
Abstract The pre-frontal cortex is the basis of the most complex mental abilities of human development. In its evaluation process, the EFECO scale provides an important contribution to assess its status. In previous investigations this scale has been studied with its configuration of 67 items, narrative focused on the deficit and evaluation of 8 executive functions. This research presents a new version of the scale, centered on its narrative in executive ability, proposed items to assess executive verification function and a summarized version of 42 items. The study included 118 healthy adults between 18 and 25 years of age (M age = 20.72, SD = 1.65). In the results it was found that the EFECO II-VC scale (modified and complete version) obtained as internal consistency α = .96 and its sub-scales internal consistency between α = .64 and .81. The EFECO II-VR scale (modified and summarized version) obtained α = .94 and its sub-scales between α = .68 and .79. The internal consistency of the factors in which the executive functions are included were adequate: the supervisory system of cognition II-VC α = .93 and II-VR α = .70, while the supervisor system of behavior II- VC α = .93 and II-VR α = .81. The correlations between the executive functions assessed with both scales were between medium and large r = .36 and .94. The work is closed discussing the clinical and scientific contribution of the modification of the EFECO scale.
RESUMEN
Resumen: La presente investigación busca evidencia científica acerca de las preferencias étnicas (PE) como alternativa para el estudio acerca del desarrollo de la identidad en niños por debajo de los 12 años. Con este propósito, se evaluaron las PE y del desarrollo cognitivo en una muestra de 388 niños de ambos sexos, 192 varones (49,5%) y 196 mujeres (50,5%), provenientes de zonas urbanas y peri urbanas de tres ciudades de Bolivia. Se postuló, en concordancia con la multidimensionalidad de las PE que, la edad, el origen étnico de los niños, las destrezas cognitivas, el entorno social y cultural en el que se desarrollan (homogéneo-heterogéneo) y el auto-reconocimiento con respecto a las características de su endogrupo, podrían ser factores de importante influencia sobre sus preferencias. Los resultados mostraron que los niños no consolidan su identidad antes de los 12 años. Sin embargo, resultó evidente que las PE evolucionan y modifican desde la infancia hasta el inicio de la adolescencia, siguiendo un patrón más o menos constante asociado con las variables anteriormente mencionadas.
Abstract: The present research seeks scientific evidence about ethnic preferences (EP) as an alternative for the study on the development of identity in children below 12 years old. With this purpose, the EP and cognitive development was evaluated in a sample of 388 children of both sexes, 192 boys (49.5%) and 196 women (50.5%) from urban and peri-urban of three cities of Bolivia. It was postulated, according with the multidimensionality of EP that, in addition to the age, the ethnicity of children, their cognitive skills, the social and cultural environment in which they develop (homogeneous-heterogeneous) and the self-recognition with respect to the in-group characteristics, could be factors with important influence in their preferences. The results showed that children do not consolidate their identity before 12 years. However, it became clear that ethnic preferences develop and change from childhood to early adolescence, following a more or less constant pattern associated with the aforementioned variables.
RESUMEN
Members of system N/A amino acid transporter (SNAT) family mediate transport of neutral amino acids, including l-alanine, l-glutamine, and l-histidine, across the plasma membrane and are involved in a variety of cellular functions. By using chemical labeling, glycosylation, immunofluorescence combined with molecular modeling approaches, we resolved the membrane topological structure of SNAT4, a transporter expressed predominantly in liver. To analyze the orientation using the chemical labeling and biotinylation approach, the "Cys-null" mutant of SNAT4 was first generated by mutating all five endogenous cysteine residues. Based on predicted topological structures, a single cysteine residue was introduced individually into all possible nontransmembrane domains of the Cys-null mutant. The cells expressing these mutants were labeled with N-biotinylaminoethyl methanethiosulfonate, a membrane-impermeable cysteine-directed reagent. We mapped the orientations of N- and C-terminal domains. There are three extracellular loop domains, and among them, the second loop domain is the largest that spans from amino acid residue â¼242 to â¼335. The orientation of this domain was further confirmed by the identification of two N-glycosylated residues, Asn-260 and Asn-264. Together, we showed that SNAT4 contains 10 transmembrane domains with extracellular N and C termini and a large N-glycosylated, extracellular loop domain. This is the first report concerning membrane topological structure of mammalian SNAT transporters, which will provide important implications for our understanding of structure-function of the members in this amino acid transporter family.
Asunto(s)
Sistema de Transporte de Aminoácidos A/química , Proteínas de Transporte de Membrana/química , Sistema de Transporte de Aminoácidos A/metabolismo , Animales , Asparagina/química , Células CHO , Línea Celular , Cricetinae , Cricetulus , Glicosilación , Humanos , Proteínas de la Membrana/química , Ratones , Mutagénesis Sitio-Dirigida , Sistemas de Lectura Abierta , Conformación Proteica , Estructura Terciaria de ProteínaRESUMEN
This study demonstrates the utility of a flow-through enzyme immobilized silica microreactor for lipid transformations. A silica micro structured fiber (MSF) consisting of 168 channels of internal diameter 4-5 µm provided a large surface area for the covalent immobilization of Candida antartica lipase. The specific activity of the immobilized lipase was determined by hydrolysis of p-nitrophenyl butyrate and calculated to be 0.81 U/mg. The catalytic performance of the lipase microreactor was demonstrated by the efficient ethanolysis of canola oil. The parameters affecting the performance of the MSF microreactor, including temperature and reaction flow rate, were investigated. Characterization of the lipid products exiting the microreactor was performed by non-aqueous reversed-phased liquid chromatography (NARP-LC) with evaporative light scattering detector (ELSD) and by comprehensive two-dimensional gas chromatography (GC x GC). Under optimized conditions of 1 µL/min flow rate of 5 mg/mL trioleoylglycerol (TO) in ethanol and 50 °C reaction temperature, 2-monooleoylglycerol was the main product at > 90% reaction yield. The regioselectivity of the Candida antartica lipase immobilized MSF microreactor in the presence of ethanol was found to be comparable to that obtained under conventional conditions. The ability of these reusable flow-through microreactors to regioselectively form monoacylglycerides in high yield from triacylglycerides demonstrate their potential use in small-scale lipid transformations or analytical lipids profiling.
Asunto(s)
Reactores Biológicos , Lípidos , Catálisis , Cromatografía de Gases , Enzimas Inmovilizadas/metabolismo , Lipasa/metabolismo , TemperaturaRESUMEN
The liver is a metabolism and transfer center of amino acids as well as the prime target organ of insulin. In this report, we characterized the regulation of system N/A transporter 3 (SNAT3) in the liver of dietary-restricted mice and in hepatocytes treated with serum starvation and insulin. The expression of SNAT3 was up-regulated in dietary-restricted mice. The expression of SNAT3 protein was detected on the plasma membrane of hepatocyte-like H2.35 cells with a half-life of 6-8 h. When H2.35 cells were depleted of serum, the expression of SNAT3 was increased. An increased concentration of insulin, however, suppressed SNAT3 expression. Interestingly, the down-regulation of SNAT3 expression by insulin was blocked by the specific phosphoinositide 3-kinase inhibitor LY294002 and mammalian target of rapamycin inhibitor, but not by MAPK inhibitor PD98059, suggesting that insulin exerts its effect on SNAT3 through phosphoinositide 3-kinase-mammalian target of rapamycin signaling. Surface biotinylation assay showed an increased level of SNAT3 on the cell surface after 0.5 h of insulin treatment, although no effect was observed after 24 h of treatment. Consistently, the transport of the substrate l-histidine was increased with short, but not long, treatment by insulin in both H2.35- and SNAT3-transfected COS-7 cells. The L-histidine uptake was inhibited significantly by L-histidine followed by 2-endoamino-bicycloheptane-2-carboxylic acid and L-cysteine and to a lesser extent by L-alanine and aminoisobutyric acid, but was not inhibited by alpha-(methylamino)isobutyric acid, implying that uptake of L-histidine in H2.35 cells is primarily mediated by system N transporters. In conclusion, differential regulation of SNAT3 by insulin and serum starvation reinforces the functional significance of this transporter in liver physiology.