RESUMEN
The present study describes, for the first time in an anuran amphibian, the nerve stimulation effects on the secretory and motor activity of the oviduct of adult females. The results reveal that in Rhinella arenarum oviducts, the epithelial and glandular secretory cells of the mucosa of the pars convoluta respond to nerve stimulation secreting the products synthetized and stored in their cytoplasm. The ultrastructural analysis showed that the cell content released is made up of granular, fibrillar and floccular material, exocytosis being the main secretory mechanism found in epithelial secretory cells, although apocrine and holocrine processes could also be observed. In contrast, in glandular cells only exocytosis processes were found. With respect to the participation of the nervous system in the motility of the duct, observations under our experimental conditions indicated that oviductal nerve stimulation promotes motor activity as manifested by a succession of coordinated contractions and relaxations that generate movements similar to peristaltic waves. These results were observed in oviducts from animals captured during the reproductive and post reproductive periods. However, it is important to note that both the secretory response and duct motility are markedly decreased during the post reproductive period of the species.
Asunto(s)
Bufo arenarum/fisiología , Membrana Mucosa/metabolismo , Contracción Muscular/fisiología , Oviductos/citología , Oviductos/metabolismo , Estimulación Eléctrica Transcutánea del Nervio/métodos , Animales , Estro/fisiología , Femenino , Actividad Motora/fisiología , Membrana Mucosa/citología , Oviductos/ultraestructuraRESUMEN
In the present study we analysed the ultrastructural characteristics of the oviductal mucosa of Leptodactylus chaquensis during the preovulatory period and immediately after ovulation. Epithelial secretory cells, ciliated cells, basal cells and glandular secretory cells are described. During the preovulatory period, the oviduct exhibits its maximum degree of development at both the epithelial and the glandular levels, with numerous secretory cells that contain a large number of secretory granules whose contents are released into the oviductal lumen by apocrine and exocytotic secretory processes. The secretory cells present throughout the oviduct display considerable variability in the characteristics of their secretory granules, which show different shapes, sizes, organization of the material contained and electron density. The different cell types are distributed following a characteristic pattern for each oviductal zone, thus creating an ultrastructural mosaic along the oviduct. During the postovulatory period, the number of secretory cells decreases and the remaining ones exhibit a marked reduction in secretory granules. Ciliated cells show a typical ultrastructural organization that is not modified throughout the reproductive cycle. Basal cells, located at the basal region of the epithelium, are characterized by their heterochromatic nuclei and electron-lucent cytoplasm, while glandular secretory cells exhibit oval, round or polyhedric granules, most of them with a prominent core. Our results, which indicate a high heterogeneity of secretory cell contents, allow us to suggest differential synthesis and secretion of specific products in each oviductal zone.