RESUMEN

A plant expression cassette system was engineered to efficiently target proteins to the default secretory pathway, allowing the expression of DNA inserts in three frames as fusion proteins containing a synthetic tobacco calreticulin cleavable signal peptide sequence, with the advantage of producing the recombinant proteins by phytosecretion. As one approach to develop a vaccine to enteropathogenic Escherichia coli (EPEC) infection, the oral immunogenicity of phytosecreted BfpA, the structural subunit A of the bundle-forming pilus, expressed at high levels (7.7% of soluble protein) in transgenic tobacco tissues, was demonstrated in BALB/c mice by the induction and detection of fecal anti-BfpA antibodies.

Asunto(s)

Proteínas de la Membrana Bacteriana Externa/inmunología , Proteínas de Escherichia coli , Vacunas contra Escherichia coli/inmunología , Proteínas Fimbrias , Nicotiana/genética , Proteínas Recombinantes de Fusión/inmunología , Vacunas Sintéticas/inmunología , Administración Oral , Animales , Anticuerpos Antibacterianos/biosíntesis , Proteínas de la Membrana Bacteriana Externa/genética , Proteínas de la Membrana Bacteriana Externa/metabolismo , Secuencia de Bases , Vectores Genéticos , Masculino , Ratones , Ratones Endogámicos BALB C , Datos de Secuencia Molecular , Plantas Modificadas Genéticamente , Proteínas Recombinantes de Fusión/metabolismo