RESUMEN
1. An investigation was conducted on the effects of aflatoxin (AFL)-contaminated diets and feed withdrawal periods from 0 to 12 h in broiler chickens at 28 d of age. Both factors can potentially affect liver colour and can cause failure at veterinary inspection in the slaughterhouse.2. A total of 240, one-d-old female Cobb 500 broiler chickens were fed a common corn-soy pre-starters (d 1-7) and then either a non-contaminated control (CON) or feed with 1 ppm AFL (AFL) from d 8 to 28. The inoculum of AFL had 792 ppb of aflatoxin B1, 35 ppb of aflatoxin B2 and 219 ppb of aflatoxin G1. On d 28, all broilers were weighed and euthanised for necropsy following three different feed withdrawal time periods (0, 6 or 12 h), in a 2 × 3 factorial arrangement.3. Body weight gain, liver weight and liver fat content decreased as feed withdrawal lengthened, whereas FCR and gallbladder weight increased (P ≤ 0.05). AFL-fed birds had reduced body weight and proportion of liver fat and increased FCR, liver and gallbladder weights (P ≤ 0.05).4. Livers from fed broilers (0 h withdrawal) showed more lightness (L*) and yellowness (b*) than livers of broilers from 6 or 12 h withdrawal (P ≤ 0.05). The L* and redness (a*) values of livers from broilers fed diets COB were lower than those from AFL fed broilers (P ≤ 0.05).5. Prolonging pre-slaughter feed withdrawal decreased liver L*, whereas feeding AFL increased liver b*. These findings can be used to support veterinary assessment in slaughterhouses as shackled birds move on line through the inspection site. Since chicken liver is a valuable organ and an indicator of animal health, attention must be paid to these differences to ensure consumer safety.
Asunto(s)
Aflatoxinas , Pollos , Alimentación Animal/análisis , Animales , Dieta/veterinaria , Femenino , Hígado , Aumento de PesoRESUMEN
The present research was conducted to assess Mn requirements of broiler breeder hens. One hundred and twenty Cobb 500 hens, 22 wk of age, were individually allocated in cages. After fed a Mn-deficient diet (22.2 ppm), hens were randomly placed in treatments having 6 increments of 30-ppm Mn. All trace minerals were from laboratory grade sources being Mn from Mn sulfate (MnSO4H2O). Treatments were fed for 4 periods of 28 d. There were no interactions between dietary Mn and period for any evaluated response (P > 0.05). Requirements of Mn for hen day egg production and settable egg production were 115.8 and 56.6 ppm and 122.1 and 63.6 ppm (P < 0.05), respectively, using quadratic polynomial (QP) and broken line quadratic (BLQ) models, whereas total eggs and total settable eggs per hen had Mn requirements estimated at 115.7 and 56.6 and 121.8 and 61.7 ppm (P < 0.05), respectively. Number of cracked, defective, and contaminated eggs decreased, whereas hatchability, hatchability of fertile eggs, eggshell percentage, and eggshell palisade layer increased when hens were fed diets having 48.5 to 168.2-ppm Mn (P < 0.05). Maximum responses for egg weight and eggshell percentage were 117.7 and 63.6 ppm as well as 131.6 and 71.0 ppm (P < 0.05), respectively, using QP and BLQ models. Breaking strength and egg specific gravity had Mn requirements estimated at 140.2 and 112.7 ppm as well as 131.3 68.5 ppm (P < 0.05), whereas eggshell palisade layer and eggshell thickness were maximized with 128.8 and 68.8 ppm and 140.2 134.2 ppm, respectively, for QP and BLQ models (P < 0.05). Maximum yolk Mn content values were obtained using 118.0- and 118.4-ppm Mn by QP and BLQ models, respectively. The average Mn requirements estimated for QP and BLQ models is 128.4 and 92.3 ppm Mn (18.7 and 13.5 mg/hen/d), respectively, which is much lower than what has been currently recommended in commercial production.
Asunto(s)
Alimentación Animal , Pollos , Dieta , Manganeso , Alimentación Animal/análisis , Animales , Dieta/veterinaria , Cáscara de Huevo/efectos de los fármacos , Femenino , Manganeso/metabolismo , Manganeso/farmacología , Distribución Aleatoria , Cigoto/efectos de los fármacosRESUMEN
1. Gradual feed restriction was applied to broilers in order to reduce growth rate and, as a consequence, gradually impacts wooden breast myopathy occurrence. Ultrasound (US) images of breast muscle in live birds were correlated with breast fillets presenting wooden breast characteristics (WB). 2. A total of 1800 Cobb × Cobb 500 slow-feathering male chicks were fed one of the six feed restriction treatments with 12 replicates of 25 birds each, in a completely randomised design. Birds were fed ad libitum or were pair-fed to 50%, 60%, 70%, 80% or 90% of normal ad libitum intakes from 8 to 49 d to provide a gradual reduction in growth rate. Ultrasound images were obtained weekly from all birds and, in parallel, one bird per pen was weekly slaughtered and the major breast muscle was weighed and WB graded as 0 (normal), 1 (mild hardening in the upper), 2 (moderate hardening in the upper and/or lower), 3 (severe hardening) and 4 (severe hardening with haemorrhagic lesions and yellow fluid). Blood was taken for analysis of enzymes related to muscle cell breakdown. 3. Feed restriction applied at 50%, 60%, 70%, 80% and 90% of the ad libitum feed intake (FI) resulted in decreased body weight gain (BWG; P ≤ 0.05). 4. From 21 to 49 d, the increasing feed restriction led to linear increases (P ≤ 0.05) in WB scores, fibre density as well as breast depth and breast echogenicity. Creatine kinase, lactate dehydrogenase and aspartate aminotransferase concentration decreased linearly when broilers were feed restricted (P ≤ 0.05). 5. Wooden breast was positively correlated with echogenicity at 21 d (r = 0.510), 28 (r = 0.531), 35 (r = 0.470), 42 (r = 0.430) and 49 d (r = 0.548) (P ≤ 0.001). The use of breast echogenicity can be an additional tool to early detect alterations related to wooden breast.
Asunto(s)
Alimentación Animal , Pollos , Alimentación Animal/análisis , Animales , Dieta/veterinaria , Masculino , Músculos Pectorales/diagnóstico por imagen , Ultrasonografía/veterinariaRESUMEN
A study was conducted to evaluate the effects of a supplemental α-amylase on energy and nutrient utilization of broiler chickens fed diets with variable amounts of corn. A total of 480 slow feathering Cobb × Cobb 500 male broilers were randomly distributed into 10 treatments with 8 replicates of 6 birds each. Birds were fed a common starter to 14 D post-hatch. The experimental diets were provided subsequently until 25 D, which were a conventional corn-soy basal diet (CS) and a corn-soy diet displaced with 40% of corn (CN). These were fed as-is or supplemented with 40, 80, 120, or 160 kilo-Novo α-amylase units (KNU)/kg. Dietary treatments were distributed factorially as a 2 × 5 arrangement (diet type vs. amylase). Samples of feed, excreta, and ileal digesta were analyzed for determination of ileal digestible energy (IDE), ME, total tract retention, and digestibility of dry matter, protein, and fat. No interactions between diet type and amylase were observed. The CN diet had lower (P < 0.05) energy utilization and nutrient digestibility when compared to the CS diet. AMEn and IDE increased (P < 0.05) by 110 and 207 kcal/kg, respectively, when CS and CN diets were supplemented with 80 KNU/kg. The amylase added to the CS diet led to quadratic increases (P < 0.05) on growth performance, IDE, AME, and AMEn, as well as in dry matter, protein, and fat digestibility. Energy utilization and crude protein digestibility were linearly increased (P < 0.05) when amylase was added to the CN diet or the extrapolated 100% of corn. In conclusion, energy utilization, digestibility of crude protein, fat, and dry matter increased with amylase supplementation in corn-soy-based diets. When amylase was tested in a complete diet having 53.6% corn, 100 and 105 KNU/kg maximized AMEn and IDE, respectively; however, the maximum energy response in the CN diet or 100% of corn was not achieved until 160 KNU/kg, suggesting an association between amylase dose optimization and dietary starch concentration.
Asunto(s)
Pollos/fisiología , Digestión , Metabolismo Energético , Nutrientes/fisiología , alfa-Amilasas/metabolismo , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales/efectos de los fármacos , Animales , Dieta/veterinaria , Suplementos Dietéticos/análisis , Digestión/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Metabolismo Energético/efectos de los fármacos , Masculino , Distribución Aleatoria , Zea mays/química , alfa-Amilasas/administración & dosificaciónRESUMEN
One hundred and twenty Cobb 500 hens, 20 wk of age, were randomly allocated into individual cages with the objective of estimating their Zn requirements. The study was composed of 3 phases: adaptation to cages (basal diet), depletion (deficient diet containing 18.7 ± 0.47 ppm Zn) for 7 wk, and experimental phases. Hens were fed diets with graded increments of Zn sulfate heptahydrate (ZnSO4·7H2O), totaling 18.7 ± 0.47, 50.3 ± 10.6, 77.3.0 ± 11.0, 110.2 ± 12.8, 140 ± 12.2, and 170.6 ± 13.2 ppm analyzed Zn in feeds for 12 wk (experimental phase). Requirements of Zn were done using quadratic polynomial (QP), broken line quadratic (BLQ), and exponential asymptotic (EA) models. In general, the non-linear statistical models were the ones that best fit the results in this study. Requirements obtained for hen day egg production and settable egg production were 83.3, 78.6 ppm and 61.4, 65.4 ppm for period of 33 to 36 wk, and 63.3, 53.1 and 60.4, 46.1 ppm for period of 37 to 40 wk, and 62.8, 52.8, and 67.7, 62.1 ppm for period of 41 to 44 wk, respectively, using BLQ and EA models. Total eggs and total settable eggs produced per hen had Zn requirements estimated as 75.7, 64.7 ppm, and 56.5, 41.5 ppm, respectively, for BLQ and EA models, whereas for alkaline phosphatase and eggshell percentage were 161.8, 124.9 ppm and 126.1, 122.4 ppm, using QP and BLQ models. Maximum responses for Zn in yolk for periods of 37 to 40 and 41 to 44 wk were 71.0, 78.1 and 64.5, 59.6 ppm, respectively, using BLQ and EA models. Breaking strength had Zn requirements estimated at 68.0 and 96.7 ppm, whereas eggshell palisade layer and eggshell thickness were maximized with 67.9, 67.9 ppm, and 67.7, 64.4 ppm, respectively, for BLQ and EA models. The average of all Zn requirement estimates obtained by EA and BLQ models in the present study was 72.28 ppm or 11.1 mg/hen/d.
Asunto(s)
Alimentación Animal/análisis , Pollos/fisiología , Reproducción/efectos de los fármacos , Zinc/metabolismo , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Dieta/veterinaria , Cáscara de Huevo/efectos de los fármacos , Yema de Huevo/química , Femenino , OviposiciónRESUMEN
Dorsal cranial myopathy (DCM), which affects the anterior latissimus dorsi (ALD) muscles of commercial broilers, is of unknown etiology, and it represents up to 6% of the partial condemnations in Brazilian slaughterhouses. This study was performed to achieve histomorphometric characterizations of the ALD muscles from male Cobb 500 broilers slaughtered at either 35 d or 42 d and to evaluate the effects of DCM on the enzymatic markers aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase (CK), and lactate dehydrogenase (LDH) and on uric acid and creatinine metabolites. Blood samples (1.5 to 3 mL) and ALD muscle fragments were collected from each carcass, all of which were processed in a commercial inline processing system. For each age, twelve macroscopically normal animals and twelve animals found to exhibit DCM were randomly selected for histomorphometric evaluation and analysis of serologic profiles. Microscopic evaluations demonstrated that the muscle fibers of those with DCM exhibited a strong presence of multifocal regenerative myodegeneration as well as a substitution of muscle tissue with connective tissue (P < 0.001) through fibrosis, thus characterizing the chronicity and hardness of the affected muscle. It is suggested that DCM is a localized muscle lesion because the detected serum levels of CK (P < 0.001), AST (P < 0.001), ALT (P = 0.01), and LDH (P < 0.001) enzymes were strongly associated with the group affected by DCM. Additional studies are needed to gain an understanding of this myopathy because it is an emerging problem in the poultry industry. In addition, it is related to DCM lesions in fast-growing broilers with the greatest slaughter weights.
Asunto(s)
Pollos , Enzimas/sangre , Enfermedades Musculares/veterinaria , Enfermedades de las Aves de Corral/patología , Músculos Superficiales de la Espalda/patología , Animales , Brasil , Masculino , Enfermedades Musculares/patología , Enfermedades Musculares/fisiopatología , Enfermedades de las Aves de Corral/fisiopatologíaRESUMEN
Two experiments were conducted to evaluate the prevalence and severity of white striping (WS) and wooden breast (WB) in breast fillets from broilers fed diets with increasing digestible Lysine (dLys) from 12 to 28 d (Exp. 1) and from 28 to 42 d (Exp. 2). Trials were sequentially conducted using one-d-old male, slow-feathering Cobb 500 × Cobb broilers, both with 6 treatments and 8 replicates. Increasing dLys levels were equally spaced from 0.77 to 1.17% in Exp. 1 and from 0.68 to 1.07% in Exp. 2. The lowest dLys diet was not supplemented with L-Lysine (L-Lys) in either one of the studies and all other essential amino acid (AA) met or exceeded current commercial recommendations such that their dietary concentrations did not limit broiler growth. Four birds per pen were randomly selected from each replication and processed at 35 and 42 d in Exp. 1 and Exp. 2, respectively. Deboned breast fillets (Pectoralis major) were submitted to a 3 subject panel evaluation to detect the presence of WS and WB, as well as to provide scores of WS (0-normal, 1-moderate, 2-severe) and WB (0-normal, 1-moderate light, 2-moderate, 3-severe). Increasing the level of dLys had a positive effect on BW, carcass, and breast weight, as well as breast yield. White striping and WB prevalences were 32.3 and 85.9% in Exp. 1 and 87.1 and 89.2% in Exp. 2. Birds fed diets not supplemented with L-Lys had the lowest average WS and WB scores (0.22 and 0.78 in Exp. 1 and 0.61 and 0.68 in Exp. 2). White striping and WB presented linear responses to performance variables in Exp. 1, whereas quadratic responses were observed for all variables in Exp. 2. In conclusion, increasing the level of dLys improved growth performance and carcass traits as well as induced the occurrence and severity of WS and WB lesions.
Asunto(s)
Pollos/fisiología , Lisina/efectos adversos , Carne/análisis , Músculos Pectorales/patología , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales/efectos de los fármacos , Animales , Pollos/crecimiento & desarrollo , Dieta/veterinaria , Suplementos Dietéticos/análisis , Lisina/administración & dosificación , Masculino , Músculos Pectorales/efectos de los fármacos , Distribución AleatoriaRESUMEN
A trial was conducted to evaluate the effects of adding an exogenous -amylase--glucanase complex produced from Bacillus amyloliquefaciens on the growth performance, carcass yield, and relative AME bioequivalence in broilers fed corn-soy diets from 1 to 40 d of age. One thousand seven hundred and fifty one-day-old Cobb x Cobb 500 slow-feathering male broilers were randomly allotted to seven treatments with 10 replicates of 25 birds each as follows: control diet (C); C diet with reduction of 60 (C-60), 90 (C-90), or 120 (C-120) kcal AME/kg; C diet with reduction of 120 kcal AME/kg and supplemented with 200 (C-120-200), 300 (C-120-300), or 400 (C-120-400) mg of the enzyme complex/kg. Each g of the enzyme complex corresponded to 200 kilo-Novo -amylase and 350 fungal -glucanase units. On d 40, eight birds were randomly taken from each pen and processed to evaluate carcass and commercial cuts yields. Percent mortality was not affected by the treatments (p > 0.05). Live performance, as indicated by BW gain (BWG) linearly decreased (p 0.05) and FCR linearly increased with the reduction in AME. Birds fed diets supplemented with the enzyme complex showed weekly improvements in BWG and FCR. There were no effects of the treatments on the yield of the carcass or of commercial cuts; however, abdominal fat was significantly lower (p 0.0343) in birds fed the C-120-400 compared to the C-120 feeding program (1.67% vs. 1.90%); all other treatments were intermediate. Average AME equivalence of the enzyme complex varied weekly. Estimations for the entire period were 40, 46, and 56 kcal for BWG and 58, 76, and 99 kcal AME/kg for FCR (p 0.001) for the diets containing 200, 300, and 400 mg enzyme complex/kg, respectively.
Asunto(s)
Animales , Carne , Carne/análisis , alfa-Amilasas/análisis , Pollos/metabolismoRESUMEN
A trial was conducted to evaluate the effects of adding an exogenous -amylase--glucanase complex produced from Bacillus amyloliquefaciens on the growth performance, carcass yield, and relative AME bioequivalence in broilers fed corn-soy diets from 1 to 40 d of age. One thousand seven hundred and fifty one-day-old Cobb x Cobb 500 slow-feathering male broilers were randomly allotted to seven treatments with 10 replicates of 25 birds each as follows: control diet (C); C diet with reduction of 60 (C-60), 90 (C-90), or 120 (C-120) kcal AME/kg; C diet with reduction of 120 kcal AME/kg and supplemented with 200 (C-120-200), 300 (C-120-300), or 400 (C-120-400) mg of the enzyme complex/kg. Each g of the enzyme complex corresponded to 200 kilo-Novo -amylase and 350 fungal -glucanase units. On d 40, eight birds were randomly taken from each pen and processed to evaluate carcass and commercial cuts yields. Percent mortality was not affected by the treatments (p > 0.05). Live performance, as indicated by BW gain (BWG) linearly decreased (p 0.05) and FCR linearly increased with the reduction in AME. Birds fed diets supplemented with the enzyme complex showed weekly improvements in BWG and FCR. There were no effects of the treatments on the yield of the carcass or of commercial cuts; however, abdominal fat was significantly lower (p 0.0343) in birds fed the C-120-400 compared to the C-120 feeding program (1.67% vs. 1.90%); all other treatments were intermediate. Average AME equivalence of the enzyme complex varied weekly. Estimations for the entire period were 40, 46, and 56 kcal for BWG and 58, 76, and 99 kcal AME/kg for FCR (p 0.001) for the diets containing 200, 300, and 400 mg enzyme complex/kg, respectively.(AU)
Asunto(s)
Animales , Carne/análisis , Carne , alfa-Amilasas/análisis , Pollos/metabolismoRESUMEN
Acidulated soy soapstock (ASS) and lecithin (LEC) are by-products from processing soy oil (SO) for human consumption, whereas glycerol (GLY) can be obtained through the transesterification of vegetable oils during the production of biodiesel. These are valuable by-products for poultry feeds that have been underutilized in many parts of the world. One study was conducted to estimate the AMEn of ASS, LEC, GLY as well as of their mixture (MIX: 85% ASS, 5% LEC and 10% GLY). Two hundred and sixty Cobb 500 female broilers of 20 days of age were housed in steel wire battery cages in a controlled temperature room for broilers. A completely randomized (energy sources x fat inclusion level) factorial design was applied, with 4 replicates of three birds per treatment. Birds were fed a corn-soybean meal control diet without supplemental fat or with the addition of 2, 4, or 6 % of the four energy sources. Total excreta collection was performed from 26 to 28 days. The AMEn values of by-products were calculated using regression analysis as well as by the difference method. The average AMEn values calculated by regression analysis were: 9,232, 7,502, 5,447 and 8,404, whereas results with the difference method were: 7,951, 6,579, 3,979 and 8,101 kcal/kg for, in both cases for ASS, LEC, GLY and MIX, respectively. It is concluded that these energy sources can be for broilers and that there are significant differences between the methods used to estimate AMEn of fats.
Asunto(s)
Animales , Fuentes Generadoras de Energía/análisis , Fuentes Generadoras de Energía/métodos , Glicerol/administración & dosificación , Lecitinas/administración & dosificación , Aceite de Soja/administración & dosificación , Aves de Corral/metabolismoRESUMEN
Acidulated soy soapstock (ASS) and lecithin (LEC) are by-products from processing soy oil (SO) for human consumption, whereas glycerol (GLY) can be obtained through the transesterification of vegetable oils during the production of biodiesel. These are valuable by-products for poultry feeds that have been underutilized in many parts of the world. One study was conducted to estimate the AMEn of ASS, LEC, GLY as well as of their mixture (MIX: 85% ASS, 5% LEC and 10% GLY). Two hundred and sixty Cobb 500 female broilers of 20 days of age were housed in steel wire battery cages in a controlled temperature room for broilers. A completely randomized (energy sources x fat inclusion level) factorial design was applied, with 4 replicates of three birds per treatment. Birds were fed a corn-soybean meal control diet without supplemental fat or with the addition of 2, 4, or 6 % of the four energy sources. Total excreta collection was performed from 26 to 28 days. The AMEn values of by-products were calculated using regression analysis as well as by the difference method. The average AMEn values calculated by regression analysis were: 9,232, 7,502, 5,447 and 8,404, whereas results with the difference method were: 7,951, 6,579, 3,979 and 8,101 kcal/kg for, in both cases for ASS, LEC, GLY and MIX, respectively. It is concluded that these energy sources can be for broilers and that there are significant differences between the methods used to estimate AMEn of fats.(AU)
Asunto(s)
Animales , Fuentes Generadoras de Energía/análisis , Fuentes Generadoras de Energía/métodos , Aceite de Soja/administración & dosificación , Lecitinas/administración & dosificación , Glicerol/administración & dosificación , Aves de Corral/metabolismoRESUMEN
Two experiments using soybean meal (SBM) or canola meal (CM) were conducted to investigate whether the choice of digestibility marker influenced the apparent ileal digestibility (AID) or standardized ileal digestibility (SID) of N and AA in diets supplemented with phytase. In each experiment, 18 barrows fitted with T-cannulas at the ileocecal junction were assigned to 3 diets consisting of a N-free diet to determine endogenous losses of N and AA, a semipurified diet (SBM in Exp. 1 or CM in Exp. 2), and the semipurified diet supplemented with phytase at 1,000 phytase units/kg. Three digestibility markers including acid-insoluble ash (AIA), chromic oxide (Cr2O3), and titanium dioxide (TiO2) were added to each diet at 3 g/kg. Each diet was fed for 7 d, consisting of a 5-d adjustment and a 2-d collection of ileal digesta. In both studies, basal ileal endogenous losses determined with Cr2O3 as a digestibility marker were lower (P<0.01) than with those determined with AIA or TiO2 digestibility markers. Using SBM as the protein source in Exp. 1, there was no interaction between phytase and digestibility marker on AID or SID of AA. The AID of N and AA in SBM using AIA as a digestibility marker tended to be lower (P<0.1) compared with Cr2O3 or TiO2 digestibility markers. Phytase supplementation increased (P<0.001) the AID of Ca and P. The use of AIA or Cr2O3 digestibility marker tended to be associated with lower (P<0.1) SID values compared with TiO2. Phytase did not affect the SID of N or any AA in SBM except for Met, for which there was an increase (P<0.05) with phytase supplementation. Using CM as the protein source in Exp. 2, there were significant interactions between digestibility marker and phytase. Phytase supplementation had effects (P<0.01) on AID or SID when Cr2O3 or TiO2 was used as the digestibility marker. With Cr2O3 or TiO2 as the digestibility marker in the CM diets, phytase supplementation increased (P<0.05) the SID of N and all AA (except Trp). There was no SID of N or AA response to phytase supplementation of CM when AIA was used as a digestibility marker. In contrast, there were no clear improvements in AA digestibility from phytase supplementation for SBM. Phytase effects on AID or SID of AA were dependent on the digestibility marker used in diets when CM was used as the protein source but not when SBM was used as the protein source. Therefore, AA digestibility response to phytase supplementation may depend on the protein being evaluated as well as the choice of digestibility marker.
Asunto(s)
Aminoácidos/metabolismo , Alimentación Animal/análisis , Fenómenos Fisiológicos Nutricionales de los Animales , Biomarcadores/metabolismo , Digestión/fisiología , Íleon/metabolismo , Sus scrofa/fisiología , 6-Fitasa/farmacología , Animales , Cateterismo/veterinaria , Cromatografía Líquida de Alta Presión/veterinaria , Compuestos de Cromo/administración & dosificación , Compuestos de Cromo/metabolismo , Dieta/veterinaria , Suplementos Dietéticos , Digestión/efectos de los fármacos , Ácidos Grasos Monoinsaturados/química , Modelos Lineales , Aceite de Brassica napus , Glycine max/química , Espectrofotometría/veterinaria , Porcinos , Titanio/administración & dosificación , Titanio/metabolismoRESUMEN
AIMS: Thirty Campylobacter jejuni strains isolated from fecal samples (n = 94; 32%) from 13 positive farms (n = 17; 76%) from commercial broiler chickens in Puerto Rico were analysed by molecular methods. METHODS AND RESULTS: Isolates were identified with multiplex polymerase chain reaction assays, tested for their antimicrobial susceptibility and characterized with pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), serotyping and bacterial cytotoxicity in mammalian cells. Isolates exhibited high resistance to vancomycin (minimum inhibitory concentration, MIC of >256 microg ml(-1)) and trimethoprim (MIC of >32 microg ml(-1)); few were resistant to clindamycin (MIC(90) 4 microg ml(-1)), erythromycin (MIC(90) 8 microg ml(-1)) and tetracycline (MIC(90) 8 microg ml(-1)); but none was resistant to azithromycin (MIC(90) 4 microg ml(-1)), ciprofloxacin (MIC(90) 1 microg ml(-1)) or gentamycin (MIC(90) 4 microg ml(-1)). Most strains restricted with SmaI, but a combination of SmaI-KpnI digestion was more discriminatory. MLST analysis yielded four sequence types (ST), and ST-2624 was the predominant one. Phylogenetic analysis revealed a high degree of recombination for glnA and pgm genes. The predominant serotypes were O:3 and O:5. Most strains had lowest cytotoxicity potential with Caco-2 cells, medium cytotoxicity with INT-407 and Hep-2 cells and high cytotoxicity with CHO cells. CONCLUSION: A low degree of antimicrobial resistance, 13 PFGE profiles, 4 ST and a large variability in cytotoxicity assays were found for these strains. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first characterization of C. jejuni strains isolated from broilers in Puerto Rico. The genetic diversity of these strains suggests that several techniques are needed for strain characterization.
Asunto(s)
Infecciones por Campylobacter/microbiología , Infecciones por Campylobacter/veterinaria , Campylobacter jejuni/clasificación , Pollos/microbiología , Microbiología de Alimentos , Enfermedades de las Aves de Corral/microbiología , Animales , Células CACO-2 , Campylobacter jejuni/efectos de los fármacos , Campylobacter jejuni/genética , Pruebas Inmunológicas de Citotoxicidad , Pruebas Antimicrobianas de Difusión por Disco , Genotipo , Humanos , Pruebas de Sensibilidad Microbiana , Puerto Rico , Serotipificación , Resistencia a la VancomicinaRESUMEN
An experiment with 1,440 male Cobb 500 and 1,440 male Ross 308 broilers (14 to 35 d of age) was conducted to investigate the effects of diets having 4 levels of digestible methionine plus cysteine (SAA) on various performance criteria at 2 dietary protein levels (20.5 and 26.0%). Two corn-soybean meal/poultry by-product basal diets were formulated to contain 3,060 kcal/kg MEn and either 20.5 or 26.0% balanced protein, and 1.12 and 1.46% digestible (according to table values) lysine, respectively. Except for SAA, the ratios between essential amino acids were kept identical in both diets according to the ideal protein concept. The ratio between digestible SAA and digestible Lys was 50%. All remaining nutrients met or exceeded NRC (1994) recommendations. Graded levels of SAA were supplemented to obtain digestible SAA to Lys ratios of 62, 69, and 77%, with 77% representing an optimized amino acid balance. Increasing the protein level clearly improved weight gain, feed conversion, breast meat yield, and abdominal fat content. Increasing SAA levels resulted in strong nonlinear or linear dose responses at both protein levels and for both strains. Regression analysis suggested that reducing digestible SAA in a balanced protein (diets with SAA:Lys of 77%) impairs performance, and that optimum SAA:Lys ratio for growing broilers might be higher than 77%, although ANOVA revealed no significant improvement with an SAA:Lys ratio higher than 69%. Responses provide evidence that optimum dietary SAA level depends on dietary protein level and should therefore be related to the protein content.
Asunto(s)
Aminoácidos Sulfúricos/administración & dosificación , Pollos/crecimiento & desarrollo , Dieta , Proteínas en la Dieta/administración & dosificación , Lisina/administración & dosificación , Fenómenos Fisiológicos Nutricionales de los Animales , Animales , Cisteína/administración & dosificación , Digestión , Masculino , Metionina/administración & dosificación , Análisis de Regresión , Aumento de PesoRESUMEN
The present study describes the determination of the bioavailability of a new commercial tablet formulation of lamivudine (CAS 134678-17-4) compared with a reference formulation. The comparative bioequivalence of the test and a reference formulation (each 3 x 150 mg) was assessed in 24 healthy volunteers by means of a randomized two-way crossover design. Prior to the study both the test and reference formulations were examined for conformation to chromatographic purity and drug content. Each volunteer received the test (T) and the reference formulation (R) with a one-week drug-free interval between administrations. The plasma concentrations of T were monitored over a period of 12 h after drug administration using a sensitive HPLC method. Pharmacokinetic parameters for T were determined from plasma concentration-time data. Statistical tests were carried out at 90% confidence intervals using a parametric method (three-way ANOVA) for AUC and Cmax, and non-parametric method for Tmax. The present study showed that both formulations were bioequivalent for the geometric mean of AUC(0-12), AUC0-infinity), Cmax, and Tmax at the 90% confidence interval. The bioavailability of the test (%) was 96.7, 93.3, 99.7, 100.3, respectively. The T:R ratio was, in each case, well within the acceptable range of 100 +/- 20%.
Asunto(s)
Fármacos Anti-VIH/administración & dosificación , Fármacos Anti-VIH/farmacocinética , Lamivudine/administración & dosificación , Lamivudine/farmacocinética , Adulto , Área Bajo la Curva , Cromatografía Líquida de Alta Presión , Estudios Cruzados , Humanos , Masculino , Estudios Prospectivos , Comprimidos , Equivalencia TerapéuticaRESUMEN
The 50% effective intraperitoneal (ip) dose of Bothrops jararaca antivenom (ED50) was assessed in mice immediately (ED50 Oh) and thirty minutes (ED50 30') after the intramuscular (im) injection of two 50% lethal dose (LD50) of Bothrops jararaca venom. The efficacy of the antivenom injected at the venom inoculation site was assessed by the inoculation of two LD50 of the venom by im route, followed immediately (ED50 Oh) and 30 minutes later (ED50 30') by administration of the ED50 of the antivenom either entirely by the ip route or 50 percent ip plus 50 percent im, at the same inoculation site. It was shown that the ED50 30' was 3 times greater, than the ED50 Oh and that the antivenom was more protective to mice (lower death rate in 48 hours) when given entirely ip. It was concluded that, in this experimental model, a higher dose of bothropic antivenom is needed when the treatment is started lately, and that there is no benefit in its administration at the venom inoculation site.
Asunto(s)
Antivenenos/administración & dosificación , Bothrops , Venenos de Crotálidos/envenenamiento , Animales , Antivenenos/farmacología , Venenos de Crotálidos/administración & dosificación , Femenino , Inyecciones Intramusculares , Inyecciones Intraperitoneales , Dosificación Letal Mediana , Masculino , RatonesRESUMEN
The efficacy of the Crotalus durissus terrificus antivenom administration by intramuscular (im) injection at the same place of the im inoculation, of the C. d. terrificus venom was evaluated in mice. In three experiments two DL50 of the venom were inoculated and the antivenom was administered in three different ways: half of the ED50 by intraperitoneal (ip) administration and half by injection, at the same place, immediately after the venom inoculation and thirty minutes after the im venom inoculation; four fifth of ED50 by ip administration and one fifth by injection, at the same place and thirty minutes after the venom inoculation. The antivenom that was administered by intraperitoneal route provided a higher protection to mice (a lower death rate in a 48 hours period) than when it was administered in parts, by intramuscular injection, at the same place of the venom inoculation (p < 0.05). Therefore, it is concluded that this should not be used in human beings bitten by snakes.