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Glial cells have been implicated in temporal lobe epilepsy in humans and in its models. Astrocytes are lost in several brain regions after acute seizures induced by pilocarpine and may suffer hyperplasia at subsequent time points. This study investigated the effect of N-methyl-(2S,4R)-trans-4-hydroxy-L-proline (NMP) on astrocytes exposed to cytotoxic concentrations of pilocarpine. Astrocytes were incubated with pilocarpine (half maximal inhibitory concentration (IC50)=31.86 mM) for 24 h. Afterwards, they were treated with NMP at concentrations ranging from 3.12 to 100 µg/mL for 24 h. Cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cytoplasmic reactive oxygen species (ROS) and mitochondrial transmembrane potential (ΔΨm) were analyzed by flow cytometry using 2',7'-dichlorofluorescein diacetate (DCFH-DA) and rhodamine-123 (Rho123), respectively. Expression of glial fibrillary acidic protein (GFAP) and voltage-dependent anion channel-1 (VDAC-1) were measured by western blot. Pilocarpine significantly decreased cell viability and mitochondrial potential and increased ROS concentration significantly by 6.7 times compared to the control. NMP concentrations ≥25 µg/mL protected astrocytes against pilocarpine-induced injury in a concentration-dependent manner. Concomitantly, NMP reduced cytoplasmic ROS accumulation to 27.3, 24.8, and 12.3% in the groups treated with 25, 50, and 100 µg/mL NMP, respectively. NMP also protected mitochondria from pilocarpine-induced depolarization. These effects were associated with improvement of pilocarpine-induced GFAP and VDAC-1 overexpression, which are important biomarkers of astrocyte dysfunction. In conclusion, the improvement of ROS accumulation, VDAC-1 overexpression, and mitochondrial depolarization are possible mechanisms of the NMP protective action on reactive astrocytes.

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Glial cells have been implicated in temporal lobe epilepsy in humans and in its models. Astrocytes are lost in several brain regions after acute seizures induced by pilocarpine and may suffer hyperplasia at subsequent time points. This study investigated the effect of N-methyl-(2S,4R)-trans-4-hydroxy-L-proline (NMP) on astrocytes exposed to cytotoxic concentrations of pilocarpine. Astrocytes were incubated with pilocarpine (half maximal inhibitory concentration (IC50)=31.86 mM) for 24 h. Afterwards, they were treated with NMP at concentrations ranging from 3.12 to 100 μg/mL for 24 h. Cell viability was assessed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Cytoplasmic reactive oxygen species (ROS) and mitochondrial transmembrane potential (ΔΨm) were analyzed by flow cytometry using 2',7'-dichlorofluorescein diacetate (DCFH-DA) and rhodamine-123 (Rho123), respectively. Expression of glial fibrillary acidic protein (GFAP) and voltage-dependent anion channel-1 (VDAC-1) were measured by western blot. Pilocarpine significantly decreased cell viability and mitochondrial potential and increased ROS concentration significantly by 6.7 times compared to the control. NMP concentrations ≥25 µg/mL protected astrocytes against pilocarpine-induced injury in a concentration-dependent manner. Concomitantly, NMP reduced cytoplasmic ROS accumulation to 27.3, 24.8, and 12.3% in the groups treated with 25, 50, and 100 µg/mL NMP, respectively. NMP also protected mitochondria from pilocarpine-induced depolarization. These effects were associated with improvement of pilocarpine-induced GFAP and VDAC-1 overexpression, which are important biomarkers of astrocyte dysfunction. In conclusion, the improvement of ROS accumulation, VDAC-1 overexpression, and mitochondrial depolarization are possible mechanisms of the NMP protective action on reactive astrocytes.

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It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 µg/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1ß and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries.

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1. Broiler breeders are subjected to qualitative or quantitative feed restrictions to prevent obesity, which causes major health and welfare problems. Diluting their feed by adding inert or low nutrient, bulky materials can reduce obesity, but the capacity of the gut needs to be determined to apply this strategy successfully. Two trials were conducted to measure the bulk capacity of Ross 308 broiler breeders prior to and after the onset of lay. The trial was completely randomised, with nine individually-caged breeders, with each cage as a replicate, totalling 189 birds per trial2. Birds were given ad libitum access to one of 21 maize-soyabean based feeds, an undiluted control or progressive dilution (10, 20, 30 and 40%) with either cellulose fibre, rice husk, sand, vermiculite or sawdust. Feeds were analysed for density, crude-, acid detergent- and neutral detergent-fibre, water-holding capacity (WHC), cation-exchange capacity and oil-holding capacity.2. In general, feed intake (scaled to body weight0.67) increased and then declined as the proportion of each diluent increased. Intake increased linearly when rice hulls and sand were used as diluents.3. Water holding capacity was the most appropriate measure to define the gut capacity of broiler breeders.4. The trial data was used to estimate the maximum-scaled feed intake (SFImax) in broiler breeders, which was 240-56.1WHC + 4.34WHC2 g/kg0.67/d.

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It was previously demonstrated that the methanol fraction of Sideroxylon obtusifolium (MFSOL) promoted anti-inflammatory and healing activity in excisional wounds. Thus, the present work investigated the healing effects of MFSOL on human keratinocyte cells (HaCaT) and experimental burn model injuries. HaCaT cells were used to study MFSOL's effect on cell migration and proliferation rates. Female Swiss mice were subjected to a second-degree superficial burn protocol and divided into four treatment groups: Vehicle, 1.0% silver sulfadiazine, and 0.5 or 1.0% MFSOL Cream (CrMFSOL). Samples were collected to quantify the inflammatory mediators, and histological analyses were performed after 3, 7, and 14 days. The results showed that MFSOL (50 μg/mL) stimulated HaCaT cells by increasing proliferation and migration rates. Moreover, 0.5% CrMFSOL attenuated myeloperoxidase (MPO) activity and also stimulated the release of interleukin (IL)-1β and IL-10 after 3 days of treatment. CrMFSOL (0.5%) also enhanced wound contraction, promoted improvement of tissue remodeling, and increased collagen production after 7 days and VEGF release after 14 days. Therefore, MFSOL stimulated human keratinocyte (HaCaT) cells and improved wound healing via modulation of inflammatory mediators of burn injuries.

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Ketamine (KET) is an N-methyl-D-aspartate (NMDA) antagonist with rapid and long-lasting antidepressant effects, but how the drug shows its sustained effects is still a matter of controversy. The objectives were to evaluate the mechanisms for KET rapid (30 min) and long-lasting (15 and 30 days after) antidepressant effects in mice. A single dose of KET (2, 5, or 10 mg/kg, po) was administered to male Swiss mice and the forced swim test (FST) was performed 30 min, 15, or 30 days later. Imipramine (IMI, 30 mg/kg, ip), a tricyclic antidepressant drug, was used as reference. The mice were euthanized, separated into two time-point groups (D1, first day after KET injection; D30, 30 days later), and brain sections were processed for glycogen synthase kinase-3 (GSK-3), histone deacetylase (HDAC), brain-derived neurotrophic factor (BDNF), and glial fibrillary acidic protein (GFAP) immunohistochemical assays. KET (5 and 10 mg/kg) presented rapid and long-lasting antidepressant-like effects. As expected, the immunoreactivities for brain GSK-3 and HDAC decreased compared to control groups in all areas (striatum, DG, CA1, CA3, and mainly pre-frontal cortex, PFC) after KET injection. Increases in BDNF immunostaining were demonstrated in the PFC, DG, CA1, and CA3 areas at D1 and D30 time-points. GFAP immunoreactivity was also increased in the PFC and striatum at both time-points. In conclusion, KET changed brain BDNF and GFAP expressions 30 days after a single administration. Although neuroplasticity could be involved in the observed effects of KET, more studies are needed to explain the mechanisms for the drug's sustained antidepressant-like effects.

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Ketamine (KET) is an N-methyl-D-aspartate (NMDA) antagonist with rapid and long-lasting antidepressant effects, but how the drug shows its sustained effects is still a matter of controversy. The objectives were to evaluate the mechanisms for KET rapid (30 min) and long-lasting (15 and 30 days after) antidepressant effects in mice. A single dose of KET (2, 5, or 10 mg/kg, po) was administered to male Swiss mice and the forced swim test (FST) was performed 30 min, 15, or 30 days later. Imipramine (IMI, 30 mg/kg, ip), a tricyclic antidepressant drug, was used as reference. The mice were euthanized, separated into two time-point groups (D1, first day after KET injection; D30, 30 days later), and brain sections were processed for glycogen synthase kinase-3 (GSK-3), histone deacetylase (HDAC), brain-derived neurotrophic factor (BDNF), and glial fibrillary acidic protein (GFAP) immunohistochemical assays. KET (5 and 10 mg/kg) presented rapid and long-lasting antidepressant-like effects. As expected, the immunoreactivities for brain GSK-3 and HDAC decreased compared to control groups in all areas (striatum, DG, CA1, CA3, and mainly pre-frontal cortex, PFC) after KET injection. Increases in BDNF immunostaining were demonstrated in the PFC, DG, CA1, and CA3 areas at D1 and D30 time-points. GFAP immunoreactivity was also increased in the PFC and striatum at both time-points. In conclusion, KET changed brain BDNF and GFAP expressions 30 days after a single administration. Although neuroplasticity could be involved in the observed effects of KET, more studies are needed to explain the mechanisms for the drug's sustained antidepressant-like effects.

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Nutritionists have been discussing whether the dietary supplementation of cyst(e)ine is required as a part of the dietary methionine (Met) in the total sulfur amino acid (TSAA) requirement to achieve optimum performance in broilers. Part of Met is converted to cysteine (Cys) to meet the Cys requirement, especially for feather growth. The TSAA requirement has been determined by using graded levels of free Met in the diet, without supplementation of free cyst(e)ine. It has also been argued that the Met to Cys ratio (Met : Cys) changes with age and even with different Met sources. The objective of this study was to evaluate the two sources of Met, while determining the proportion of Met and Cys in total dietary TSAA that optimize the performance of broilers. A performance assay was carried out in a factorial arrangement (5 × 2) using 1080 broilers from 42 to 56 days of age fed diets having different dietary proportions of Met and Cys (44 : 56, 46 : 54, 48 : 52, 50 : 50 or 52 : 48) while maintaining the same dietary TSAA in the diets. Two synthetic Met sources (dl-Met or l-Met) were used for each of the diets with different dietary Met : Cys ratios. Twenty-one broilers of the same age were fed the diets 44 : 56, 48 : 52 and 52 : 48 by supplementing the diet with L-(15N) Met or L-(15N2) Cystine to study the metabolism of TSAA. No differences were observed between Met sources for feed intake, BW gain and feed conversion ratio (FCR; P > 0.05); however, FCR was numerically improved at 50 : 50 Met : Cys. Regarding TSAA utilization, the conversion of Met to Cys increased with increase in Met : Cys ratios, but the concentration of Met intermediates decreased. Broiler chickens responded to different dietary proportions of sulfur amino acids by altering their sulfur amino acid metabolism, and diets containing 50 : 50 Met : Cys is recommended for broilers of age 42 to 56 days.

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Himatanthus drasticus (Mart.) Plumel belongs to the Apocynaceae family and the latex from its trunk bark (Hd) is known as "janaguba milk". This latex is widely used in Northeast Brazil, mainly in the Cariri region, for its gastroprotective, anti-inflammatory, and antitumor properties. The objective of this study was to investigate a triterpene-rich fraction (FJNB) from H. drasticus latex on acute models of nociception and inflammation and to clarify its mechanisms of action. Wistar rats or Swiss mice were subjected to the carrageenan-induced paw edema test or the formalin test, respectively, after the acute oral treatment with FJNB. The inflamed paws from the carrageenan-induced paw edema and formalin tests were processed for histological and immunohistochemical assays, respectively. The results were analyzed by ANOVA and considered significant at P<0.05. FJNB (10 mg/kg) decreased the paw edema by 25% at the 3rd h after the carrageenan injection. Indomethacin, used as reference, inhibited the paw edema by 59% at the same time-point. In the formalin test, FJNB inhibited the 1st phase by 27, 49, and 52% and the 2nd phase by 37, 50, and 67%, at the doses of 1, 5, and 10 mg/kg, respectively. In addition, FJNB significantly inhibited the expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and the inflammatory cytokine tumor necrosis factor (TNF)-alpha. The histone deacetylase (HDAC) expression and the transcription factor nuclear factor kappa (NF-kB) were also inhibited at the same doses. In conclusion, the FJNB inhibitory actions on iNOS, COX-2, TNF-α, HDAC, and NF-kB could be involved with the drug anti-inflammatory activity.

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The aim of this study was to evaluate the therapeutic effects of ultrasound (US)-mediated phonophoresis alone or in association with diclofenac diethylammonium (DCF) administered topically in animal models of inflammation. A pre-clinical, prospective, and randomized experimental study of quantitative and qualitative nature was carried out. Phonophoresis was performed using a therapeutic ultrasound apparatus in two distinct models of acute inflammation. Edema was induced by an intraplantar injection of carrageenan and measured by plethysmography. The Hargreaves test was used to evaluate the antinociceptive activity and investigate the action of phonophoresis on tumor necrosis factor (TNF)-α production. A histological analysis with hematoxylin-eosin was used to evaluate tissue repair, and the expression of COX-2 was determined by immunohistochemical analysis. At the peak of inflammatory activity (3 h), treatment with US, US+DCF, and DCF significantly reduced edema formation compared to the control group. Treatment with US+DCF was more effective than treatment with US alone at both analyzed times. In the analysis of the antinociceptive activity, the treatments significantly increased the latency time in response to the thermal stimulus. Histopathological analysis revealed a reduction of the inflammatory infiltrates and immunohistochemistry demonstrated that the association was effective in reducing COX-2 expression compared to the control group. The association of DCF with US produced anti-inflammatory and antinociceptive effects in rat models of inflammation, which may be associated with inhibition of COX-2 and TNF-α production.

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The aim of this study was to evaluate the therapeutic effects of ultrasound (US)-mediated phonophoresis alone or in association with diclofenac diethylammonium (DCF) administered topically in animal models of inflammation. A pre-clinical, prospective, and randomized experimental study of quantitative and qualitative nature was carried out. Phonophoresis was performed using a therapeutic ultrasound apparatus in two distinct models of acute inflammation. Edema was induced by an intraplantar injection of carrageenan and measured by plethysmography. The Hargreaves test was used to evaluate the antinociceptive activity and investigate the action of phonophoresis on tumor necrosis factor (TNF)-α production. A histological analysis with hematoxylin-eosin was used to evaluate tissue repair, and the expression of COX-2 was determined by immunohistochemical analysis. At the peak of inflammatory activity (3 h), treatment with US, US+DCF, and DCF significantly reduced edema formation compared to the control group. Treatment with US+DCF was more effective than treatment with US alone at both analyzed times. In the analysis of the antinociceptive activity, the treatments significantly increased the latency time in response to the thermal stimulus. Histopathological analysis revealed a reduction of the inflammatory infiltrates and immunohistochemistry demonstrated that the association was effective in reducing COX-2 expression compared to the control group. The association of DCF with US produced anti-inflammatory and antinociceptive effects in rat models of inflammation, which may be associated with inhibition of COX-2 and TNF-α production.

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Himatanthus drasticus (Mart.) Plumel belongs to the Apocynaceae family and the latex from its trunk bark (Hd) is known as "janaguba milk". This latex is widely used in Northeast Brazil, mainly in the Cariri region, for its gastroprotective, anti-inflammatory, and antitumor properties. The objective of this study was to investigate a triterpene-rich fraction (FJNB) from H. drasticus latex on acute models of nociception and inflammation and to clarify its mechanisms of action. Wistar rats or Swiss mice were subjected to the carrageenan-induced paw edema test or the formalin test, respectively, after the acute oral treatment with FJNB. The inflamed paws from the carrageenan-induced paw edema and formalin tests were processed for histological and immunohistochemical assays, respectively. The results were analyzed by ANOVA and considered significant at P<0.05. FJNB (10 mg/kg) decreased the paw edema by 25% at the 3rd h after the carrageenan injection. Indomethacin, used as reference, inhibited the paw edema by 59% at the same time-point. In the formalin test, FJNB inhibited the 1st phase by 27, 49, and 52% and the 2nd phase by 37, 50, and 67%, at the doses of 1, 5, and 10 mg/kg, respectively. In addition, FJNB significantly inhibited the expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and the inflammatory cytokine tumor necrosis factor (TNF)-alpha. The histone deacetylase (HDAC) expression and the transcription factor nuclear factor kappa (NF-kB) were also inhibited at the same doses. In conclusion, the FJNB inhibitory actions on iNOS, COX-2, TNF-α, HDAC, and NF-kB could be involved with the drug anti-inflammatory activity.

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A digestibility assay was conducted in order to determine the digestibility coefficients (DC) of amino acids of feed ingredients in cecectomized adult roosters. In total 48 cecectomized adult roosters were used to assess the coefficient of digestibility of 7 ingredients: corn, soybean meal, soybean concentrate, corn gluten meal, wheat bran, peanut meal, and feather meal. Each ingredient was replicated six times and a group of 6 rooster was used to measure endogenous amino acid losses. After 48 hours of fasting, the roosters were tube fed 20g of ingredients tested twice during a day. At 12 hour-intervals excreta and endogenous losses were collected and immediately freeze-dried for further chemical analysis. At the end of the assay, excreta were weighed and samples of ingredients, excreta, and endogenous losses were pooled and analysed for dry matter, nitrogen and amino acid content. The coefficients of indispensable amino acids for the most feed ingredients assessed in the current research were similar to published literature like AMINODat®5.0 and the 4th edition of the Brazilian Tables for Poultry and Swine, except feather meal, in which only digestible Trp and Thr content were similar to literature. Particularly, the coefficients for some amino acids like Arg and Gly exhibited large discrepancies from literature in almost all the feed ingredients assessed herein. In general, using cecectomized adult roosters proved to be a reliable technique to assess the digestibility of feed ingredients used in poultry diets.

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A digestibility assay was conducted in order to determine the digestibility coefficients (DC) of amino acids of feed ingredients in cecectomized adult roosters. In total 48 cecectomized adult roosters were used to assess the coefficient of digestibility of 7 ingredients: corn, soybean meal, soybean concentrate, corn gluten meal, wheat bran, peanut meal, and feather meal. Each ingredient was replicated six times and a group of 6 rooster was used to measure endogenous amino acid losses. After 48 hours of fasting, the roosters were tube fed 20g of ingredients tested twice during a day. At 12 hour-intervals excreta and endogenous losses were collected and immediately freeze-dried for further chemical analysis. At the end of the assay, excreta were weighed and samples of ingredients, excreta, and endogenous losses were pooled and analysed for dry matter, nitrogen and amino acid content. The coefficients of indispensable amino acids for the most feed ingredients assessed in the current research were similar to published literature like AMINODat®5.0 and the 4th edition of the Brazilian Tables for Poultry and Swine, except feather meal, in which only digestible Trp and Thr content were similar to literature. Particularly, the coefficients for some amino acids like Arg and Gly exhibited large discrepancies from literature in almost all the feed ingredients assessed herein. In general, using cecectomized adult roosters proved to be a reliable technique to assess the digestibility of feed ingredients used in poultry diets.(AU)

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This study was conducted to determine the optimal standardized ileal digestible arginine to lysine (SID Arg:Lys) ratio for Japanese quails in the egg-laying phase. A total of two hundred forty-five 35-week-old Japanese quails (181 ± 1.30 g initial body weight) were randomly assigned to five treatment groups with seven replicates of seven quails. Graded levels of L-Arginine were added to a basal diet in order to produce five SID Arg:Lys ratios (101, 106, 111, 116 and 121%). Collected data were analyzed as one-way ANOVA and optimal ratio was estimated using polynomial regression model (linear and quadratic) based on performance traits. Statistical differences were considered for p<0.05. Graded SID Arg:Lys ratios did not affect performance traits assessed. Based on results, the SID Arg:Lys ratio of 101% is sufficient to warrant proper performance of Japanese quails in the egg-laying phase.

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This experiment was conducted to evaluate the effects of dietary nitrogen-corrected apparent metabolizable energy (AMEn) levels on meat-type quail performance and carcass traits from 1 to 14d of age. A total of 1120 not sexed meat-type quails were randomly distributed to seven treatments, with eight replicates with 20 quails each. A basal corn and soybean meal-based diet was formulated to meet or exceed quail nutritional requirements, except for ME. Graded levels of soybean oil were added to the basal diet in replacement, to sand, to obtain dietary treatments (2,600; 2,700; 2,800; 2,900; 3,000; 3,100 and 3,200 KcalAMEn/kg diet). Data were analyzed as one-way ANOVA and optimum AMEn levels were estimated using polynomial regression model. Increasing in dietary AMEn levels elicited a linear decrease (p0.05) by AMEn. Based on the results, the dietary AMEn level that warrants adequate performance and carcass traits of meat-type quails from 1 to 14d of age is 2,820 Kcal/kg diet.

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This experiment was conducted to evaluate the effects of dietary nitrogen-corrected apparent metabolizable energy (AMEn) levels on meat-type quail performance and carcass traits from 1 to 14d of age. A total of 1120 not sexed meat-type quails were randomly distributed to seven treatments, with eight replicates with 20 quails each. A basal corn and soybean meal-based diet was formulated to meet or exceed quail nutritional requirements, except for ME. Graded levels of soybean oil were added to the basal diet in replacement, to sand, to obtain dietary treatments (2,600; 2,700; 2,800; 2,900; 3,000; 3,100 and 3,200 KcalAMEn/kg diet). Data were analyzed as one-way ANOVA and optimum AMEn levels were estimated using polynomial regression model. Increasing in dietary AMEn levels elicited a linear decrease (p<0.01) in feed intake and nutrient intake (AMEn, protein and lysine). Quail weight gain and final body weight exhibited a quadratic response (p<0.03) to increased AMEn levels, being both optimized at 2820 KcalAMEn/kg diet. Graded AMEn levels elicited a linear increase (p<0.01) in carcass dry matter and fat content, whereas moisture content was linearly decreased (p<0.01). The protein content of the carcasses was not influenced (p>0.05) by AMEn. Based on the results, the dietary AMEn level that warrants adequate performance and carcass traits of meat-type quails from 1 to 14d of age is 2,820 Kcal/kg diet.(AU)

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This study was conducted to determine the optimal standardized ileal digestible arginine to lysine (SID Arg:Lys) ratio for Japanese quails in the egg-laying phase. A total of two hundred forty-five 35-week-old Japanese quails (181 ± 1.30 g initial body weight) were randomly assigned to five treatment groups with seven replicates of seven quails. Graded levels of L-Arginine were added to a basal diet in order to produce five SID Arg:Lys ratios (101, 106, 111, 116 and 121%). Collected data were analyzed as one-way ANOVA and optimal ratio was estimated using polynomial regression model (linear and quadratic) based on performance traits. Statistical differences were considered for p<0.05. Graded SID Arg:Lys ratios did not affect performance traits assessed. Based on results, the SID Arg:Lys ratio of 101% is sufficient to warrant proper performance of Japanese quails in the egg-laying phase.(AU)

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ABSTRACT This study was conducted to determine the optimum standardized ileal digestible isoleucine to lysine (SID Ile:Lys) ratio for meat-type quails from 15 to 35 d of age. Three hundred fifty not-sexed meat-type quails (Coturnix coturnix coturnix) were randomly assigned into five treatments, with seven replicates of 10 quails each. An isoleucine-deficient corn-soybean meal-based diet was formulated and graded supplemented with L-isoleucine (99%) to obtain diets containing SID Ile:Lys ratios of 55,61, 67, 73, and 79%. Data were analyzed as one-way ANOVA and optimum SID Ile:Lys was estimated by polynomial (linear and quadratic) regression. Statistical differences were considered when p 0.05. Quail performance from 15 and 21 d and 15 and 28 d of age was not affected by the treatments. From 15 to 35 d of age, body weight gain and body weight exhibited a quadratic response to increasing dietary SID Ile:Lys ratios, and were optimized at 66 and 67% SID Ile:Lys, respectively. Feed conversion ratio was not influenced by SID Ile:Lys ratios in any of the phases assessed herein. Based on the results, the optimum SID Ile:Lys ratio for meat-type quails from 15 to 35 d of age is 67%.

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ABSTRACT This study was conducted to determine the optimum standardized ileal digestible isoleucine to lysine (SID Ile:Lys) ratio for meat-type quails from 15 to 35 d of age. Three hundred fifty not-sexed meat-type quails (Coturnix coturnix coturnix) were randomly assigned into five treatments, with seven replicates of 10 quails each. An isoleucine-deficient corn-soybean meal-based diet was formulated and graded supplemented with L-isoleucine (99%) to obtain diets containing SID Ile:Lys ratios of 55,61, 67, 73, and 79%. Data were analyzed as one-way ANOVA and optimum SID Ile:Lys was estimated by polynomial (linear and quadratic) regression. Statistical differences were considered when p 0.05. Quail performance from 15 and 21 d and 15 and 28 d of age was not affected by the treatments. From 15 to 35 d of age, body weight gain and body weight exhibited a quadratic response to increasing dietary SID Ile:Lys ratios, and were optimized at 66 and 67% SID Ile:Lys, respectively. Feed conversion ratio was not influenced by SID Ile:Lys ratios in any of the phases assessed herein. Based on the results, the optimum SID Ile:Lys ratio for meat-type quails from 15 to 35 d of age is 67%.(AU)

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